YI ZHANG, PH.D.

***** ****** **** ** ************, Maryland 20878

240-***-**** actk6c@r.postjobfree.com

SENIOR SCIENTIST

Accomplished, motivated, energetic, and detail oriented scientist with 7 years of postdoctoral experience conducting research spanning molecular biology, cell biology, biochemistry, genetics and microbiology.

Advanced expertise in cell culture with cell lines used in protein production, cell-based assays, development of stable cell lines, and various molecular biology techniques.

Extensive experience overseeing protein engineering, expression, purification, and characterization, including the development of bio-therapeutic recombinant proteins.

Excellent ability to critically analyze data, work effectively with cross-functional groups, and write/prepare technical reports, presentations and manuscripts.

Understanding of regulatory requirements.

Research & Development Project Management Assay Development, Qualification/Validation

Laboratory Set-Up Equipment Maintenance & Acquisition Research Team Mentorship & Training High-Impact Presentations Literature Review Data Documentation, Analysis & Review

Technical Reporting Experimental Trouble-Shooting Multi-Tasking Time Management

Cell Biology: Mammalian Cell Culture (e.g. CHO, HEK293) Cell Transfection and Selection Single Cell Cloning Cell-based Assays Cell Line Maintenance FACS Immunostaining Immunohistochemistry Cell Migration & Metastasis Fluorescence / Widefield / Confocal Microscopy Ligand Target Identification Cellular Pharmacology Signal Transduction Pathways

Molecular Biology: CRISPR/Cas9 Techniques gRNA and Donor DNA Design and Constructions for Gene Knockout/Knockin PCR/qPCR DNA/RNA Purification Restriction Enzyme Digestion Recombineering Gene Cloning Gene Sequencing Southern Analysis Viral Vector and Plasmid Construction / Manipulation Microarray Primer Design Yeast-2-Hybrid

Biochemistry: In Vitro Biochemical & Biophysical Techniques ELISA Biacore Chromatography HPLC SDS-PAGE Western Blot Protein-Protein Interactions DNA/RNA-Protein Interactions Protein Labeling Gel-Shift Assays Co-Immunoprecipitation Separation Science Techniques ATKA Explorer UF / DF Centrifugation Equipment

PROFESSIONAL EXPERIENCE

CHILDREN’S NATIONAL MEDICAL CENTER, WASHINGTON DC 2015 (Dec.) - Present

Scientist: Lead researches to establish human genetic disease models in Drosophila. Using CRISPR/CAS9 techniques to target genes to create various Drosophila knockout/knockin mutant lines, and further establish the biological connection between a human mutated gene with an animal phenotype.

NATIONAL CANCER INSTITUTE, Bethesda, Maryland 2009-2015

Postdoctoral Scientist: Lead studies in cancer epigenetics to identify factors influencing genome stability. Managed experiments in gene cloning/targeting; cell culture; recombineering; protein engineering, expression, and purification; molecular interaction; protein assays. Conducted gene/protein functional studies, cell based assays, and genetic analysis. Developed research documentations/protocols. Wrote and prepared manuscripts for publication in peer-reviewed journals. Presented findings at conferences. Supervised and trained junior scientists and students.

Discovered novel telomere capping protein complex MTV. Introduced efficient expression and purification of MTV by designing novel cell expression system.

Developed cell lines expressing MTV complex.

Designed in vitro and in vivo assays identifying 8 amino acids essential for interaction and function of MTV, later adopted by laboratories in China.

Developed novel expression vectors allowing efficient expression / purification of unstable TEA protein.

Elucidated action of mechanism of protein complex MTV by in vitro biochemical assays.

Published 2 scientific papers from key research initiatives and prepared 2 manuscripts for future submission. Reviewed 15 submitted papers for 5 high-profile journals.

DALHOUSIE UNIVERSITY, Halifax, Canada 2005-2009

Postdoctoral Fellow, Department of Biochemistry & Molecular Biology 2009: Directed independent research into generating novel anti-angiogenic proteins using recombinant protein engineering techniques. Performed molecular biology assays including PCR/qPCR, gene cloning, gel electrophoresis, and sequencing.

Developed several vectors for expression of novel anti-angiogenic proteins in mammalian cells.

Research Assistant 2005-2009: Investigated functions of protein Annexin A2 and S100A10, and performed anti-angiogenic studies. Conducted in vitro biochemical and cell-based assays; designed and screened therapeutic anti-angiogenic recombinant proteins. Performed different protein purifications using chromatography and HPLC instruments. Performed immune assays to track expression level of Annexin A2 in patient samples. Managed laboratory operations; acquired and maintained equipment, planned laboratory operations, and trained students. Developed experimental documentation. Wrote scientific proposals and papers. Delivered presentations.

Discovered cancer cell-killing protein DT385 and identified triggering methods using intracellular trafficking and biochemical assays.

Identified anti-angiogenic properties of endotoxin; identified anti-angiogenic protein P22 through sequence analysis, literature analysis, data review, and functional study.

Pinpointed key role of S100A10 in cancer cell migration and metastasis through molecular and cell based assays investigating gene functions.

Published 2 scientific papers in high-profile peer-reviewed journals.

Established new Halifax lab after transfer from Calgary University.

UNIVERSITY OF CALGARY, Calgary, Canada 2002-2005

Research Assistant: Conducted in-depth research into cancer-prevention properties of biotherapeutic proteins. Oversaw research into Annexin A2 and RNA binding.

SELECT EXPERIENCE PRIOR TO 2002 (DETAILS UPON REQUEST)

UNIVERSITY OF WAIKATO, Visiting Scholar, Hamilton, New Zealand: Characterized thermophilic bacteria from hot springs, dairy factories, and composts in Antarctic region and New Zealand.

CHINESE ACADEMY OF SCIENCE, Associate Scientist, Chengdu, China: Led 4-person team in conducting pharmaceutical research. Established laboratory and experimental protocol. Supervised laboratory team in synthesizing di-peptides.

Formed partnership with Quanxin factory to develop bifidobacteria powder.

Chosen to lead National Key Project to identify anaerobic bacteria that generate ethanol using cellulose as substrate.

EDUCATION

Doctoral Degree in Biochemistry & Molecular Biology

Dalhousie University, Halifax, Canada

Master of Science in Cellular, Molecular, and Microbial Biology

University of Calgary, Calgary, Canada

Master of Science in Microbiology

Chengdu Institute of Biology, Chinese Academy of Science, Chengdu, China

Bachelor of Science in Biochemistry

Sichuan University, Chengdu, China

NATIONAL INSTITUTE OF HEALTH PROFESSIONAL DEVELOPMENT & TRAINING

Introduction to Medical Genetics II Cancer Screening Principles of Clinical Pharmacology

Current Concepts in Clinical Molecular Genetics & Molecular Diagnostics

Theoretical & Applied Bioinformatics RNA-Sequencing Workshop & Data Analysis

Bioinformatic Analysis of Next-Generation Sequencing Data Workshop

Principles of Pharmacokinetic Data Analysis: Modeling & Simulation

Data Mining the Cancer Genome Atlas (TCGA) Statistical Analysis of Research Data

Writing & Publishing Scientific Papers Scientists Teaching Science

TEACHING EXPERIENCE

Problem-Based Learning Tutor: Dalhousie University College of Pharmacy 2007-2008

Teaching Assistant: Biology, Biochemistry: Dalhousie University 2006-2007

Biochemistry Instructor, Canadian Institute of Traditional Chinese Medicine, 2005

Teaching Assistant: Microbiology, siRNA Technology: University of Calgary, 2001, 2002, 2004

MANUSCRIPT REVIEW

PLoS ONE Neoplasma International Journal of Biochemistry and Biophysics

Journal of Molecular Biology Research The International Journal of Biological Markers

International Journal of Biochemistry and Biophysics (Editorial Board Member)

Journal of Molecular Biology Research (Editorial Board Member)

AWARDS

Cancer Research Training Program (CRTP) Award: 2006 & 2008, Dalhousie University

Alberta Cancer Board Scholarship, 2004 & 2005, University of Calgary

MDSC Graduate Research Scholarship, 2003 & 2004, University of Calgary

Bettina Bahlsen Memorial Graduate Scholarship, 2002, University of Calgary

SELECT PUBLICATIONS

Zhang Y., et al. MTV, a telomeric ssDNA-binding complex, protects Drosophila telomeres and recruits retro-transposon to chromosome ends. Submitted. Journal: Dev. Cell.

Zhang Y., Schreiner W. Rong YS. (2014). Genome manipulations with bacterial recombineering and site-specific integration in Drosophila. Methods in Molecular Biology. 1114:11-24.

Morciano P., Zhang Y., Wesolowska, N., Cenci G., Rong YS. (2013). A hypomorphic mutation reveals a stringent requirement of the ATM checkpoint protein in telomere protection during early cell divisions in Drosophila. G3:Genes Genomes Genetics. 3: 1043-1048.

Zhang, Y., Schulte, W, Pink, D., Phipps, K., Zijlstra, A, Lewis, J. and Waisman, D.M. (2010). Sensitivity of cancer cells to truncated diphtheria toxin. PLoS ONE 5(5):e10498.

Kwon, M., MacLeod, T. J., Zhang, Y., and Waisman, D.M. (2005). S100A10, annexin A2, and annexin A2 heterotetramer as candidate plasminogen receptors. Front Biosci. 10:300-25.

Fournier, M.*, Zhang, Y.*, Wildschut J. D., Voordouw, J. K., Schriemer, D. C. and Voordouw, G. (2003). Function of oxygen resistance proteins in the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough. J Bacteriol. 185: 71-79. (* These authors contributed equally to this work.)

SELECT PRESENTATIONS

Zhang, Y. and Yikang Rong. (2012). A new Drosophila telomere capping complex binding to ssDNA (Oral presentation). CECB (The Center of Excellence in Chromosome Biology) workshop. National Institute of Health (NIH), Bethesda. June 20, 2012

Zhang, Y., Gao, G. and Rong, Y. (2011). A new capping complex for the protection of Drosophila telomeres (Oral presentation). Telomeres & Telomerase Conference in Cold Spring Harbor Laboratory, May 3-7, 2011

Zhang, Y., Lewis, J. and Waisman, D.M. (2008). P22 and p22-diphtheria toxin conjugate as antiangiogenic molecules (poster). Nova Scotia Cancer Research Symposium, November 5, 2008

Complete List of Publications & Presentations Available Upon Request.

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