Research Construction

Jia Wu

************@*******.*** 626-***-****

**** ***** **, *** ****, CA 95148

WORK EXPERIENCE

1.Lin-Zhi International Inc. September 2014-Present

Position: Research Associate (Immunologist/R&D)

Developed and formulated monoclonal antibodies for the company’s ready to use bioassays--single analytic homogenous enzyme immunoassays (EIA) for drug of abuse (DOA) testing in urine: barbital assay and tricyclic antidepressant (TCA) assay.

The skills have been performed professionally include:

a. ELISA: the enzyme-linked immunosorbent assay; b. Gene sequencing and monoclonal antibody phage display; c. qPCR, qRT-PCR, and error-prone PCR, d. mouse work, bacteria culture and cell culture; e. enzyme (G6PDH) and hapten activation and conjugation, f. polyethylene glycol cell (PEG) fusion and electrical cell fusion between spleen cells (B cells) and myeloma cells; g. performance of EIA tests on Hitachi 717 and AU480/680.

Participated in hapten design and immunogen preparation

Managed laboratory inventory and arrangement of R&D meetings.

2.Research at Dr. Michael Kahn (PI)'s Lab and Dr. Andre Ouellette (Co-PI)'s Lab 2012-2014

Master Thesis: Wnt/β-Catenin/p300 Induced Transcription is Critical for the Differentiation and Maintenance of Paneth Cells.

Professionally performed a variety of techniques both in vitro and in vivo including PCR, qRT-PCR, immunoblotting, cell culture, bacterial cultures, immunohistochemistry and animal dosing, and dissection.

3.Live IVF Center: 2011-2012

Test blood hormone level (estrogen, progesterone, LH, FSH and beta-hCG) by using TOSOH 600II.

Provision of free consultations regarding informing infertility, clinical procedures, pharmaceutical drugs, and preparation of blood sample for send-out tests.

SUMMARY OF QUALIFICATIONS

Molecular Biology Techniques:

RNA and DNA isolation

cDNA library construction and screening

cDNA synthesis & DNA pull-down assay

Chromatin immunoprecipitation (ChIP),

PCR, real-time qPCR, and DNA gel electrophoresis

Primer construction and cloning

Nuclear Magnetic Resonance Spectroscopy

GenBank: BLAST, PSI/PHI-BLAST

Protein-DNA binding Assay

Infrared Spectroscopy Instrument & Spectrophotometers

Protein Biochemistry Techniques:

Protein Isolation

Protein gel electrophoresis

Immunofluorescence

Immunoprecipitation

Co-immunoprecipitation

Immunohistochemistry

Western blot

HPLC

Histochemistry Techniques:

H&E staining

Paraffin sections processing, embedding, and microtomy

Alcian blue staining

Protein-DNA binding assays

Phloxine and tatrazine staining

Chromatography

Additional Skills and Techniques:

Proficient with anatomy and embryology of mouse, Microsoft Word, Excel, and PowerPoint, and biostatistics (SPSS, Stata 11, and R program)

Biligual : fluently in both English and Chinese (Mandarin)

Jia Wu

************@*******.*** 626-***-****

2715 Klein Rd, San Jose, CA 95148

EDUCATION:

University of Southern California 2012-2014

Master of Science, Biochemistry and Molecular Biology GPA: 3.6

GRE Score: 1470 / 1600

University of California, Los Angeles 2008- 2010

Bachelor of Science, Biochemistry GPA: 3.2

COURSEWORK

Graduate level biochemistry, recent advances in research on sickle cell disease and sickle hemoglobinopathy, graduate level cell biology, the structure of scientific revolution, infection and host response, molecular genetics, principles of biostatistics, regulation of pharmaceutical, chemistry manufacturing and control, and introduction to risk management.

OTHER RESEARCH PROJECTS:

Double Mutants of Lac Repressor Gene Going Through Cloning, Expressing and Purifying processes to Test the Protein Characterizations by Associating with Lac Operator Gene

Identify, Quantitate and Characterize the Phospholipid That Gets Translocated during Platelet Activation and Study the Mechanism of De-activation

Self Assessment of Dietary Habit based on the standards of California Nutrient.

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