Diane Barth-Baus

***** ******* ****

Brook Park, Ohio 44142

216-***-**** home

216-***-**** cell

acsz13@r.postjobfree.com

Cleveland State University

Education:

Bachelor of Science degree in biology

Graduation in December of 1990 cum laude

• Extensive background in molecular biology and serological techniques. Qualifications:

• Twenty years experience in an academic research laboratory.

• Participate in data analysis and analytical thinking of results proposing conclusions or new experimental designs.

• Development of new techniques to be used in the research laboratory.

• Participate in the development of Standard Operating Procedures for the laboratory.

• Knowledgeable in various biomedical disciplines.

• Sustain chemical solutions and samples inventory.

• Handling and shipping of biological and biohazardous samples.

• Maintain radioactive isotope surveys and records as well as acted as primary interface for Case Department of Occupational and Environmental Safety for all radioactive and chemical reporting and audits.

• Participate in research objectives including grant and book chapter authoring.

• Proficient at laboratory management including budget, capital equipment justification and purchase.

• Oversee and develop other personnel in the laboratory. Conduct training and oversight of laboratory procedures and practices.

Molecular Biology:

Skills:

• Cell Culture techniques for NIH-373, HEK 293, MEL, HCT-116, and SF9 cells.

• Aseptic Techniques.

• Yeast culturing, cloning and protein purification.

• Radioactive isotope work.

• DNA/RNA manipulation (annealing, ligation and labeling).

• DNA cloning, plasmid purification and restriction enzyme digestion.

• Protein isolation from cell lines and FPLC.

• Transcription of DNA into RNA.

• Translation of RNA into proteins.

• 2-dimensional gels.

• Isolation of RNA from cell lines.

• Isolation of ribosomal subunits and proteins from cell lines in sucrose gradients.

• Genomic DNA and RNA isolation from human tissues.

• Preparation of the random primer labeled DNA probes.

• Poly (A) + RNA enrichment by oligo-dT column chromatography.

• Northern blot, Western blot and Southern blot analysis.

• Designing DNA and RNA molecular constructs for experiments.

• Site Directed Mutagenesis and Polymerase Chain Reaction.

• Electrophoresis Mobility Shift Assays of DNA and protein structures

• Infection and production of virus in baculovirus cells. Computer:

• Adobe Photoshop, Microsoft Word, Microsoft PowerPoint, Microsoft Excel

• Imaging software Gel Pro 5000/7500 and Image quant version 5.2 and 7.1 Employment:

Case Western Reserve University January 2010 – May 2015 Department of Pharmacology

Responsibilities:

• Established laboratory for new primary investigator, included purchase equipment, trained new personnel, and produced standard operating procedures for good laboratory practices.

• Maintained radioactive isotope surveys and records as well as acted as primary interface for Case Department of Occupational and Environmental Safety for all radioactive and chemical reporting and audits.

• Shipped biological and hazardous materials.

• Maintained all logs and records for samples tested in the laboratory.

• Laboratory Manager of 2 post-doctoral candidates, 4 graduate students and 1 undergraduate student.

Research Assistants on the following projects:

• Studied the Shelterin Complex that binds to telomeres.

• Incorporated non-native nucleotides into telomeres.

• Determined if the non-native nucleotides have been incorporated into the telomeres and if this has hindered their elongation.

• Studied the effects of the incorporated non-native nucleotides with addition of the Shelterin Complex.

• Studied a stop in the processivity of telomere with deletion of nucleotide.

• Developed baculovirus clones of the Shelterin Complex to use for protein purification.

Publications:

Lobo GP, Amengual J, Baus D, Shivdasani RA, Taylor D, von Lintig J. Genetics and diet regulate vitamin A production via the homeobox transcription factor ISX. (2013) J Biol Chem. Mar 29; 288(13): 9017-27. Corriveau M, Mullins MR, Baus D, Harris ME, Taylor DJ. Coordinated interactions of multiple POT1-TPP1 proteins with telomere DNA

(2013) J Biol Chem. Jun 7; 288(23):16361-70.

Case Western Reserve University February 2004- January 2010 Department of Biochemistry

Responsibilities:

• Involved in large shared equipment purchase, preventative maintenance contracts and purchase of parts needed for shared equipment for the Department of Biochemistry at Case Western Reserve University.

• Sustained all eukaryotic initiation factors, DNA, and RNA samples used in laboratory.

• Maintained radioactive isotope surveys and records as well as acted as primary interface for Case Department of Occupational and Environmental Safety for all radioactive and chemical reporting and audits.

• Managed laboratory supplies and equipment; acted as key individual for maintaining proper inventory, stocking and equipment, replacement and repair needs.

• Involved in submission of grants to the National Institutes of Health.

• Participated in the authoring of publications.

• Laboratory manager of 3 post-doctoral candidates, 2 graduate students, 1 technician, and 3 undergraduate students.

Research Assistant on the following projects:

• Studying cap-dependent and cap-independent translation.

• Constructing bicistornic and monocistronic clones from known cellular and viral IRESs.

• Studying the effects of addition and reduction of eukaryotic initiation factors on bicistronic and monocistronic mRNAs.

• Studying the effects of proteins with upstream start sites by the addition of eukaryotic initiation factors.

• Studying eIF2A as an alternative pathway for translation.

• Determining the post-translation modification in eIF2A under normal and stressed conditions.

• Purification of eIF2A from Saccharomyces cerevisiae. Publications:

Komar AA, Gross SR, Barth-Baus D, Strachan R, Hensold JO, Goss-Kinzy T, Merrick WC

Novel characteristics of the biological properties of the yeast Saccharomyces cerevisiae eukaryotic initiation factor 2A. (2005) J Biol Chem Apr 22:280 (16): 15601-11.

Honda K, Smith MA, Zhu X, Baus D, Merrick WC, Tartakoff AM, Hattier T, Harris PL, Siedlak SL, Fujioka H, Liu Q, Moreira PI, Miller FP, Nunomura A, Shimohama S, Perry G

Ribosomal RNA in Alzheimer disease is oxidized by bound redox-active iron.

(2005) J Biol Chem Jun 3; 280(22):20978-86

Kapasi P, Chaudhuri S, Vyas K, Baus D, Komar AA, Fox PL, Merrick WC, Mazumder B

L13a blocks 48S assembly: role of a general initiation factor in mRNA-specific translational control. (2007) Mol Cell. Jan 12:25(1):113-26 Merrick WC, Barth-Baus D

Use of reticulocyte lysates for mechanistic studies of eukaryotic translation initiation. (2007) Methods Enzymol.; 429: 1-21

Reineke LC, Cao Y, Baus D, Hossain NM, Merrick WC. Insights into the role of yeast eIF2A in IRES-mediated translation. (2007) PLoS One.: 6(9):e24492

Barth-Baus D, Bhasker CR, Zoll WL, Merrick WC.

Influence of translation factor activities on start site selection in six different mRNAs. Translation 1: e24419 (2013).

Case Western Reserve University December 2002 - February 2004 Department of Molecular and Microbiology

Responsibilities:

• Preserved all cell line samples and DNA samples for research studies.

• Maintained radioactive isotope surveys and records as well as acted as primary interface for Case Department of Occupational and Environmental Safety for all radioactive and chemical reporting and audits.

• Managed laboratory supplies and equipment acted as key individual for maintaining proper inventory, stocking and equipment, replacement and repair needs.

• Laboratory manager of 3 post-doctoral candidates and 2 graduate students. Research Assistant on the following projects:

• Constructed cell culture clones using the LTR of HIV for a Tet on/off system.

• Constructed cell culture clones using Luciferase DNA to determine the efficiency of tat on the TET on/off system.

Case Western Reserve University October 1991 - November 2002 Department of Hematology and Oncology

Responsibilities:

• Performed experiments under guidance of the principal investigator.

• Developed 2-dimensional gel system for analysis of poly(A)-binding protein.

• Maintained radioactive isotope surveys and records.

• Maintained running budget on grants.

• Laboratory manager of 2 post-doctoral candidates and 1 research assistant. Research Assistant on the following projects:

• Determined the effects of inducers on the expression of the poly(A)-binding protein.

• Determined the role of the poly(A)-binding protein in regulating gene expression in MEL cells.

• Identified a 49-kDa polysome protein and the analysis of its function.

• Determined the differences in protein representation in the 80S and polysomal subunits of translating ribosomes.

• Worked on isolating an antibody for poly(A)-binding protein. Publications:

Hensold JO, Stratton C, Barth-Baus D, and Galson D Expression of the transcription factor, Spi-1 (PU.1), in differentiating murine erythroleukemia cells Is regulated post-transcriptionally. Evidence for differential stability of transcription factor mRNAs following inducer exposure (1996) J Biol Chem Feb16; 271(7):3385-3391.

Hensold JO, Barth D, Stratton CA

RNA polymerase II inhibitor, 5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole

(DRB) causes erythroleukemic differentiation and transcriptional activation of erythroid genes. (1996) J Cell Phys Jul; 168(1):105-13. Hensold JO, Barth-Baus D, Stratton CA

Inducers of erythroleukemic differentiation cause messenger RNAs that lack poly(A)-binding protein to accumulate in translationally inactive, salt-labile 80 S ribosomal complexes. (1996) J Biol Chem Sep 20; 271(38):23246-54. Hensold JO, Stratton CA, Barth D

The conserved 5'-untranslated leader of Spi-1 (PU.1) mRNA is highly structured and potently inhibits translation in vitro but not in vivo.

(1997) Nucleic Acids Res. Jul 15; 25 (14) 2869-76. Kroll SL, Barth-Baus D, Hensold JO

The carboxyl-terminal domain of the granulocyte colony-stimulating factor receptor uncouples ribosomal biogenesis from cell cycle progression in differentiating 32D myeloid cells. (2001) J Biol Chem. Dec 28; 276(52):49410-8 Barth-Baus D, Parrott L, Hensold JO, Myerson H, Templeton DL, Landreth G S6 phosphorylation-independent pathways regulate translation of 5'-terminal oligopyrimidine tract-containing mRNAs in differentiating hematopoietic cells.

(2002) Nucleic Acids Res May 1:30(9):1919-28

Horton LE, Bushell M, Barth-Baus D, Tilleray VJ, Clemens MJ, Hensold JO p53 activation results in rapid dephosphorylation of the eIF4E-binding protein 4E- BP1, inhibition of ribosomal protein S6 kinase and inhibition of translation initiation. (2002) Oncogene Aug 8; 21(34):5325-34

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