SKILLS SUMMARY:
Administrative:
Principal Investigator of the Czech Ministry of Health Grant Project. - “The usage of FISH methods and echocardiography for the complex diagnostic system of congenital heart diseases”. Czech Republic. 2000-2002.
Collaborator in the European Union Grant Project “Fetal Nucleated Red Blood Cells in Maternal Blood during Pregnancy: First Czech Experience”. Czech Republic. 2000-2002.
Organizer of the All-Czech Workshop “PRINs and LSI techniques used in prenatal screening of the most frequent aneuploidies”. Czech Republic. 1997.
Supervisor of the Laboratory of Molecular Genetics and Prenatal Diagnostics, Masaryk hospital, Czech Republic. 1993-2002.
Supervisor of the FISH laboratory. Training of technologists, implementation and oversight of proficiency testing, quality control and quality improvement programs for the clinical laboratory. CSI Labs, Alpharetta, GA, USA 2008
FISH Team Leader. Expert level of FISH analysis (Hematological Panels, PET-ALK, PET-HER-2, Urovision); educating
and training technologists (workshops, Journal Clubs, personal coaching); introduced six successful quality improvement
proposals*.
Advanced knowledge of Macintosh/PC: MS Office (Word, Excel, Access, Power Point), Minitab, Statgraphics, Corel Draw, and Photoshop. Strong skills with relative databases, statistical analysis, professional presentations, graphic design.
Excellent knowledge of Russian, Kazakh, Turkmen and Czech languages; working knowledge of Slovak and Ukrainian languages.
Laboratory Analysis/Techniques:
Molecular Genetics assays: PCR, Electrophoresis (Thin Layer, Gel Electrophoresis), RFLP, Blotting.
Trace chemical analysis: HPLC, Spectrometry, Densitometry, Fluorometry, Centrifugation.
Cytogenetics, Molecular Cytogenetics: Bright field, Dark field, Phase-, DIC-, Nomarski contrast, Polarization and Fluorescent Microscopy (FISH, PRINS, SKY, MFISH, Urovision, ALK, Her-2) Cytogenetic analysis (Various cell cultures set up/harvest).
EXPERIENCE (USA):
Team Leader – full time
October 2008 – January 2012 - March 2015:
Genzyme 4310 East Cotton Center Blvd st.#120 Phoeniz AZ 85040, Labcorp 5005 South 40th street, Phoenix AZ 85040
Laboratory of FISH.
FISH analysis of Hematological and PET probes/panels. FISH wet lab. Designed and performed validation studies for new FISH probes. Contributed in gathering and statistical analysis of FISH scored data for the new probes using various approaches (Gaussian, Inversed Beta, Binomial statistics). Kept detailed and accurate records of validation studies results.
Journal Clubs and training workshop coordinator: contributed towards daily training of the personnel with the laboratory equipment and assays in use, including educational seminars and lectures.
FISH Supervisor – full time
March 2008 – September 2008: CSI-Lab. 11525 Park Wood Circle Alpharetta, GA, 30005.
Reported clinical results, hired and managed an experienced technical staff. Responsible for the ongoing performance of clinical tests in accordance with all ISO, CLIA, CAP regulations and responsible for the implementation and oversight of proficiency testing, quality control and quality improvement programs for the clinical laboratory.
Technologist III, Senior Technologist – full time
September 2002 – February 2008: Genzyme Genetics 4310 East Cotton Center Blvd st.#120 Phoenix AZ
85040.
Laboratory of FISH.
FISH analysis of Hematological, Urovision and PET probes/panels. FISH wet lab. Responsible for validation studies
design and statistical analysis of FISH data for the new probes by using Binomial, Inverse Beta and Gaussian approaches. Responsible for the detailed and accurate records of validation analysis results. Journal Club’s holder.
EDUCATION:
Doctor of Medicine (MD)
September 1977 – May 1984
The Moscow State University of Medicine, Medical-Biological Department. Diploma dissertation submitted to the 2nd Medical Institute, Acad. of Med. Sciences in 1984. Thesis: "T-Activin interaction with target-cells". Supervisor: DBS V.Y.Orion.
Ostrovitanova 1A, Moscow, Russian Federation.
Master’s Degree in Medical Genetics
November 1993 – May 1995
The Institute of Postgraduate Studies for Physicians and Pharmacologists
IPVZ, Ruská 85, 100 05 Prague 10. Czech Republic.
Master’s Degree in Molecular Cytogenetics (European University Diploma)
March – May 2000
Lab. of Histology-Embryology-Cytogenetics. Faculty of Medicine Univ. Montpellier1. Supervisors of practice Prof. J.P.Bureau, Dr. J. Chiesa.
Avenue Kennedy 30907, France. Nimes Cedex 2.
ASCP (American Society for Clinical Pathology) certificates:
CG(ASCP)CM – October 2003; MB(ASCP)CM – November 2003; DLM(ASCP)CM – June 2005.
MEMBERSHIP:
-Czech Medical Chamber 1993 – present
-European Cytogenetics Association (ECA). 1997-present
-American Society of Human Genetics 2006-present
-ASCP - present
LANGUAGES:
Kazakh, Turkmen, Russian, Czech, Slovak, English
PERSONAL:
Married (two children.). Citizen of the USA.
EXPERIENCE (OVERSEAS):
Supervisor of the laboratory. – full time.
February 1993 – September 2002:
The laboratory of molecular genetics and prenatal diagnostics. The department of medical genetics, The hospital named after Masaryk T.G. in Ústí n. Labem. Soc.péče 66, 401 13, Ústí n. Labem, Czech Republic
Scientific worker – full time
February 1992 – January 1993:
"SPOLCHEMIE a.s." Department of Trace Chemical Analysis. Ústí n. Labem. Czech Republic.
Scientific worker – full time
May 1986 – December 1991:
All Union Cancer Research Center. Institute of Experimental Diagnosis and Therapy of Tumors. Kashirskoe shosse 16, Moscow Russian Federation.
Assistant Professor - full time
September 1984 – May 1986:
Department of Normal Physiology. Ashabad, Turkmenistan.
1.Jyoti P Chaudhuri, Sultan Karamanov, Prabakaran Paulraj, John R McGill and Joachim-U Walther:
Identification of Parental Chromosomes Involved in Translocations BCR-ABL, t(9;22), and PML-RARA, t(15;17).
Anticancer Res 28:3573-3578 (2008).
2.Jyoti P Chaudhuri, Sultan Karamanov, Prabakaran Paulraj, John R McGill and Joachim-U Walther:
Genomic Karyotyping by Identifying Parental Chromosomes in Metaphase Spreads Tested with Translocations
BCRABL,
t(9;22), and PML-RARA, t(15;17). MS to Anticancer Res 2007.
3.Jyoti P Chaudhuri, Li Song, Liliana Scott, Sultan Karamanov and John R McGill: Orientation of Tri- and Polysomic
Chromosomes reflect Intranuclear Order. Europ J Hum Genet (in press) June 2007.
4.J.P Chaudhuri, S. Karamanov, J-U. Walther: Entstehung und Bedeutung von Ungerdaen Ploidien in Krebszellen,
(Origin and Impact of Uneven Polyploidies in Cancer) 19. Tumorzytogenetischen Arbeitstagung, Magdeburg,
Germany, 2006.
5.Structural Chromatin Features imply Genomic Separation and Epigenetic Activities Jyoti P Chaudhuri,1-3 Sultan
Karamanov,3 Matthew Battaglia,3 Carol Berger,3 and Joachim-U Walther1
European Human Genetic Conference, Munich, 12-15 June 2004, Abstract: 04-A-828 ESHG, Poster Board: PO242
6.Chaudhuri JP, Battaglia M, Karamanov S, Berger C, Stephenson C, Walther J: Structural chromatin features in
cytogenetic preparations imply genomic separation and epigenetic activities. Europ J Human Genet 12: s134, 2004.
7. S.Karamanov, D.Novotná, Š.Vilimová, J.Novaková Combination of Optimized PRINS and Spectral MFISH Protocols
– Cheap and Fast Detection of Chromosomal Rearrangements in Prenatal Diagnosis. Fetal Diagn Ther 2001;16, 456
8. Karamanov S, Homola J, Kofer J, Lastuvkova J, Satrapa V. Interstitial tandem duplication of 6p: a case with de
novo partial trisomy (6)(p21.1p21.3) Poster presentation. The 3-d Conference of European Cytogenetics Association.
Paris, France, July 2001.
9. Hromadnikova I, Bendukidze N, Karamanov S, Sedlackova L, Stechova K, Houbova B, Mrstinova M, Kofer J,
Stejskal D, Macek M, Vavrinec J Chapter: Fetal Nucleated Red Blood Cells in Maternal Blood during Pregnancy: First
Chech Experience. Monography: Fetal Cells and Fetal DNA in Maternal Blood. New Developments for a New
Millenium. Editors Hahn,S; Holzgreve,W(Basel)Karger,2001
10. Hromadníková I, Karamanov S, Mršttinová M, Štechová K, Kofer J, Houbová B, Macek M.:
Fetální jaderné erytrocyty cirkulující v periferii gravidních žen jako neinvazivní zdroj detekce pohlaví fetu. Ceská
gynekologie, 65, 2000, 4,219-223.
11. Chromosomální mikrodelece 22q11 v populaci detí s konotrunálními malformacemi srdce.
S.Karamanov, J.Homola,J.Kofer,M.Soukupová,V.Harmaš,R.Skalická,H.Povová
Ceskoslovenská pediatrie 1999, 1.,31-34
12. Screening chromosomálních mikrodelecí 22q11 v populaci detí s VCC – kasuistika dvou syndromu.
S.Karamanov,J.Homola,J.Kofer,M.Sokupová,V.Harmaš,H.Povová,R.Skalická
31.Výrocní symposium cytogenetické sekce Biol. spolecnosti CAV 1998
13. DNA diagnostika mutací genu CFTR – zámery a dosavadní výsledky
S.Karamanov,J.Kofer,J.Pajerek,M.Soukupová,R.Skalická,H.Povová
6.Pediatrický den 1998.
14. Cystická fibroza – populaaní screening v severoceském regionu.
S.Karamanov,J.Kofer,J.Homola,J.Laštuvková,R.Skalická,I.Tumová
5. Pediatrický den. 1997.
15. Naše zkušenosti s FISH metodou.
S.Karamanov,J.Kofer,J.Laštuvková,M.Wilimská
29.Výrocní symposium cytogenetické sekce Biol. spolecnosti CAV 1996.
16. Our experience in FISH at routine practice. S.Karamanov,J.Kofer,J.Laštuvková.M.Wilimská,R.Skalic PECO UCROMIC Congress, Prague Sept.,1996
Proposal of 2003
IMPATH Phoenix AZ.
Co-denaturation method for the large volume FISH lab.
S.Karamanov
Proposal of 2006
Genzyme Genetics, Phoenix AZ.
Construction of a Panel of Chromosome Specific Oligonucleotide Padlock Probes for In Situ Genotyping of Human DNA Molecules of Clinical Significance.
S. Karamanov, J. Chaudhuri, E. Kroman, C. Berger, J. McGill, D. Stegeman
Proposal of 2007
Genzyme Genetics, Phoenix AZ.
Universal FISH slides Pretreatment (Hot Ethanol Pretreatment).
S.Karamanov
Proposal of 2011
Genzyme Genetics, Phoenix AZ.
Microdirect harvest method for «QNS» FISH samples.
S.Karamanov
Proposal of 2011 ( In Service)
Genzyme Genetics,Phoenix, AZ.
MS Excel program in cytogenetics laboratory use.
S.Karamanov
Proposal of 2012
The Scoring of BoneMarrow samples in Multiple Myeloma.
S.Karamanov
Journal club of January 2003
PRINS and SKY. Principals of the Appoaches
S. Karamanov
Journal club of January 2003
Congenital Heart Diseases and Related Syndromes
S. Karamanov
Journal club of March 2003
Human Sex Differentiation
S. Karamanov
Journal club of 2005
In Situ genotyping of individual DNA molecules by target-primed rolling circle amplification of padlock probes
S. Karamanov
Journal club of September 2006
Universal pretreatment of FISH slides
S. Karamanov
Journal club of April 2007
Imprinting. Epigenetic control of genes expressions
S. Karamanov
Journal club of January 2009
Diagnosis dependent FISH scoring
S. Karamanov
Jornal club of March 2009
BCR/ABL and FIP1L1/PDGFRA fused genes testing
S. Karamanov
Journal club of October 2009
FISH scoring in cancer – the evolution in concepts
S. Karamanov
Journal club of July 2012
The scoring of Bone Marrow smears samples diagnosed with plasma cells abnormalities
S. Karamanov
Journal club of August 2013
Early FISH testing in non-small cell lung cancer for appropriate ALK-directed therapy
S. Karamanov
Journal club of February 2014
Liquidation of illiteracy in integrated analysis of paraffin embedded tissue slides
S. Karamanov
John McGill, Ph.D.
Director at Miraca life science
Tel: +1-602-***-****
Email:acs87n@r.postjobfree.com
Jaenenne Skierkowski, Ph.D.
Director at Miraca life science
Tel: +1-480-***-****
Email:acs87n@r.postjobfree.com
Lucie Guidot, DLM(ASCP)CM, CG(ASCP)CM
Laboratory manager at Quest Labs
Tel: +1-602-***-****
Email: acs87n@r.postjobfree.com
Christine F. Stephenson, Ph.D., FACMG
Clinical Cytogeneticist
Expert Genetics
Tel: +1-480-***-****
Email: acs87n@r.postjobfree.com
Hager Darrel CG(ASCP)
Team Leader technologist
Lab Corp
Tel: +1-760-***-****
Mandy A Gomez CG(ASCP)
Senior technologist
Lab Corp
Tel: +1-623-***-****
Cover letter
Dear Madam/Sir,
Your posting for a position indicated requirements that closely match my background and expertise. I have enclosed my resume to provide a summary of my qualifications and background for your review.
Throughout my career I have maintained the highest performance standards with a diverse range of administrative functions, which are clearly illustrated by my past successes. As a Principal investigator of the Grant Project of the Health Ministry of Czech Republic., I streamlined operations and reduced costs by consolidating operations and negotiating competitive rates with service providers. Additionally, in the USA while working as an Senior technologist to a supervisor of the FISH lab I facilitated the implementation of a new LIS that significantly increased the company’s effectiveness.
With excellent organizational and communication skills, an outstanding work ethic, and the ability to work well in both team-oriented and self-directed environments, I am positioned to exceed your expectations. I would welcome an opportunity to meet with you to discuss my qualifications and candidacy in further detail. Thank you for your consideration.
Sultan Karamanov