Western blot, Cell culture,PCR,Python, Linux, ELISA, FACS

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Hong Zhao

*** ****** ***, **** ***, San Jose, CA, 95134

Email: *********.*@*******.***

Tel: 408-***-****

EDUCATION

Rutgers University, New Jersey, USA

Master of Science Cell & Developmental Biology GPA: 3.75/4.0 Expected Jan 2016 Shanghai Jiao Tong University, Shanghai, China

Master of Medicine Pharmacy GPA: 3.80/4.0 Sep 2011 – Mar 2014 Shanghai Jiao Tong University, Shanghai, China

Bachelor of Science Pharmacy GPA: 3.56/4.3 Sep 2007 - July 2011 SKILLS

Laboratory skills

Western blot, Cell culture, Histological staining, Cell transfection, PCR, ELISA, Animal experiments (tissue dissection, behavior test), FACS, Immunohistochemistry, Flow cytometry Computer skills

Python, Linux, Microsoft Office (Word, Excel, PowerPoint), SPSS, EndNote, Photoshop, Prism, Matlab SELECTED COURSES

Fundamentals of Molecular Biosciences, Experimental Methods in Molecular Biosciences Pharmacology (course and lab), Pharmaceutics (course and lab), Pharmaceutics Analysis (course and lab), Medicinal Chemistry, Clinical Pharmacy, Biotechnological Drugs, bioinformatics Pattern Recognition

Open course:

Data Structures; Algorithms; Algorithms for DNA sequencing; Design and Analysis of Algorithms WORKING EXPERIENCE

IMS Consulting Group, Shanghai, China Internship Jan 2014 – Jun 2014 Project 1: Exploration of the feasibility of IMS Partnership Program Business Plan

• Performed desk research to seek merger and acquisition market and trends in Chinese local pharmaceutical companies

• Screened potential partner companies by total sales, therapy areas and products pipeline

• Approached potential buyers by making channels to connect them (international conference, personal network, publications, government leads, etc.)

Project 2: Study of gastric cancer market and patient potential in China for Bayer

• Performed desk research to build GC market landscape in China

• Conducted pilot key opinion leaders interviews to understand treatment and unmet medical needs

• Summarized all findings and strategic implications to Bayer entry into GC market identify opportunities RESEARCH EXPERIENCE

Study of the relationship between population size and two loci linkage disequilibrium Department of Genetics, Rutgers University Dr. Andrew Kern May 2015 – June 2015

• Generated the frequency of locus under various population size based on neutral model using software tool in C

• Visualized the distribution of simulation data with scatter plot in python Study of different polymers’ performance for nerve regeneration 2

Department of Chemistry, Rutgers University Dr. Joachim Kohn Feb 2015 – Apr 2015

• Made scaffolds, fiber mat and flat film with 3 different polymers varying in hydrophobicity

• Tested the growth of schwann cells on polymers and flat film with medium hydrophobicity performed best Exploration of GDH and SIRT4’s effect neuron morphology Department of Neuroscience, Rutgers University Dr. Bonnie Firestein Nov 2014 – Jan 2015

• Performed brain dissection on rat embryos and isolated hippocampus for neuron culture

• Constructed GDH and SIRT4 plasmids with retrovirus vector

• Transfected GDH and SIRT4 in to neurons by calcium phosphate transfection method Analysis of the profiles of patients with Schizophrenia Department of Genetics, Rutgers University Dr. Linda Brzustowicz Sep 2014 – Nov 2014

• Implemented a software tool that finds common SNPs in 10 patients profiles in python Exploration of the role of caspase-6 in tau truncation at D421 mediated by ROS School of Pharmacy, Shanghai Jiao Tong University Dr. Ming Yin Jan 2012 – Dec 2013

• Treated HEK 293 cells with H2O2 and verified whether it could induce tau truncation at D421 by western blot and caspase activity assay

• Identified the levels of active caspase-6 and detected caspase-6 activity by western blot

• Treated transfected cells with caspase-3 and caspase-6 inhibitors and detected the levels of tau truncation at D421 by western blot

Establishment of new AD transgenic mouse model with senescence accelerated background School of Pharmacy, Shanghai Jiao Tong University Dr. Ming Yin Nov 2010 – Jun 2011

• Crossed transgenic mice (mice models of Alzheimer’s Disease) for the research, including APP/PS1, Tau P301L, and APP/PS1/Tau 3x transgenic mice and genotyped by PCR

• Detected cognitive impairment of different groups of mice by Morris Water Maze test and locomotive behaviors by Open Field

• Identified the pathological characteristics of different groups of mice by histological staining and immunohistochemistry assay

HONORS AND AWARDS

SMC Scholarship Shanghai Jiao Tong University 2012 – 2013 Excellent Graduate Student Scholarship Shanghai Jiao Tong University 2012 – 2013 Guanghua Scholarship Shanghai Jiao Tong University 2009 – 2010 Academic Excellence Scholarship Shanghai Jiao Tong University 2007 – 2009 SELECTED PUBLICATIONS

• H. Zhao1, K. Lok, Mi. Yin, W. Zhao*. Oxidative stress and Alzheimer's disease. Chinese Journal of Gerontology. Accepted for publication.

• H. Zhao1, W. Zhao, K. Lok, Z. Wang*, M. Yin*. A synergic role of caspase-3 and caspase-6 in tau truncation at D421 induced by H2O2. Cellular and Molecular Neurobiology, 34(2014) 369-378

• K. Lok1, H. Zhao, H. Shen, Z. Wang, A. Sun, W. Zhao*, M. Yin*. Characterization of the APP/PS1 mouse model of Alzheimer's disease in senescence accelerated background. Neuroscience letters, 557

(2013), 84-89.

• W.1, K. Lok1, H. Zhao, A. Sun, M. Yin*. Establishment and evaluation of transgenic models of Alzheimer's disease with senescence accelerated background. Molecular Neurodegeneration, 7(Suppl 1): S34, 2012.

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