Research Associate
Resume:
Trusha Parikh
************@*****.***
WORK EXPERIENCE
Chemist II/ Associate Scientist at AbbVie biotherapeutics June 2015 - present
Formulations department
Working on stability, DOE and clinical studies to support formulation development. Use of several analytical techniques such as: HPLC (SEC, CEX, Protein A oxidation), Gel chip assay, Dynamic Scanning Colormeritry (DSC), UV spectrophotometry, Viscosity measurements, Dynamic Light Scattering (Zetasizer) to evaluate the stability of mAbs -Analysis of data using Empower and compiling data to excel.
Contract Research Associate (Kelly Scientific) April 2015
Plasmid amplification and purification (maxiprep), DNA digestion and gel electrophoresis
Research Associate at Durect May - November 2013
Pharmaceutical Research and Development
Worked on formulation development for peptides and proteins, sustained in vitro release using depot technology, analytical testing of the formulations for stability and potency using RPHPLC (Agilent), Developed ELISA assay for peptide and successfully analyzed PK samples.
Research Associate at Genentech May 2012 - May 2013
Drug delivery group
-Conducted in vitro drug release experiments with the ocular medical device to evaluate its release performance.
-Successfully analyzed large number of samples using UV-Vis, Bradford as per GLP guidelines and generated in vitro release profiles for different lots
-Used Chemstation and Excel to generate and compile data.
Associate Scientist I at Corium International September 2011 - May2012
Analytical R &D department
Perform stability studies on proteins formulated in microneedles at different temperature and time points, evaluate protein binding from serum samples and characterize mAbs as per GMP guidelines.
-Performed buffer extraction and analyzed samples using SDS PAGE, BCA assay, UV, SEC, IEC and RP using HPLC . -Successfully optimized ELISA for protein binding, generated data using softmax and totalchrom, trained employees on SEC, SDS-PAGE and ELISA.
Intern at Genentech June - August 2011
Hybridoma development in Antibody Engineering department
Evaluation of various adjuvants to increase immune response and to implement in vivo electroporation for antibody development.
-Performed Transformation procedures to scale up DNA, maintain cell lines, performed in vivo immunizations (ID, IP), in vivo electroporation, in vivo bioimaging, ELISA and FACS.
-Successfully generated data using Flowjo, M3vision and compiled data using prism.
Intern at Genentech June - August 2010
Hybridoma development in Antibody Engineering department
Project involved comparative study on DNA immunization using different delivery systems, use of in-vivo electroporation to increase efficiency of current immunization techniques.
- Performed DNA immunizations and in-vivo bioluminescence imaging for protein expression at various timepoints, FACS analysis to detect mAb titer.
- Successfully generated data using M3vision and FACS analysis.
SKILLS AND TECHNIQUES
Analytical chemistry, molecular and cell biology, immunology
-HPLC (SEC, RP, CEX)
-SDS-PAGE/Gel chip assay
-Spray Drying
-Lyophilization
-In vivo/vitro bioimaging
-Transfection (Transient)
-Protein extraction/purification
-ELISA
-Western blot
-DNA scale up
-Bradford/BCA assay
- Tissue culture -Isolation/purification of plasmid
-Agarose gel Electrophoresis
-Hybridoma production for mAb
-FACS
-Immunization: IP, IV, SC, ID
EDUCATION
San Jose State University, Masters in Biological sciences (GPA 3.8) May 2011
Classes studied: 1) Immunological techniques (Lab includes mice immunization, production of hybridoma secreting monoclonal antibodies, further screening and cloning, purification and characterization of mAb), 2) Bioinformatics,
3) Seminar in Molecular Biology and Microbiology, 4) Immunology class & Lab, 5) Molecular and cell biology Lab, 6) Graduate studies in Biology, 7) Flow cytometry 8) Virology
M.Sc. Pharmacology and Biotechnology January 2007
Sheffield Hallam University (United Kingdom)
Classes studied: DNA technology, Cell biology, Biology of Infectious Disease, Molecular pharmacology, Molecular biotechnology and Research methods and dissertation on DNA vaccines and tuberculosis.
B.Sc. Microbiology July 2005 M. G. Science College, Gujarat University (India)
PROJECTS SUMMARY
Dr. Tzvia Abramson’s lab at SJSU October 2009
Experiment on Immunomodulation by Bordetella species in whooping cough disease.
Research project at Sheffield Hallam University under Dr. Rowena Bunning Summer 2006
Studies on production of ADAMTS 9 by Human Neuroblastoma cells (SHSY5Y)
COMPUTER SKILLS
-Efficiently use Microsoft Word, Excel and Powerpoint.
-Familiar with R language for statistical analysis.
COMMUNICATION SKILLS
-Excellent verbal and written communication skills: Have given presentations & written SOPs.
-Have worked as a part time sales customer representative for 2 years at a call center in UK.
HONORS, AWARDS & EXTRACURRICULAR ACTIVITIES
-Awarded 1st prize for a poster presentation on HIV at Sheffield Hallam University (UK) 2006.
-Volunteered at a Medical Health awareness camp at Bhuj, India, 2008.
-Volunteered at the International Special Olympics, Ahmedabad, India, December 2004.
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