Assistant Quality Control
Resume:
MY TRINH NGUYEN
Lowell, MA *****
*******.********@*****.***
U.S Citizen
EDUCATION:
B.S in Biological Science, May 2002, University of Mass-Lowell
TECHNICAL EXPERIENCE:
Corning Biosciences, MA 03/2013-current
BD Biosciences, MA (Corning) 03/2006-06/2011
Senior Biologics Technician
QC testing: bioassays to test all reagents products ( Transportocells, ITS, Nu Serum, IL2, ECGS)
Assisting and collaborating in multiple tasks including cell culture, quality control, and administration
Preparation of MatriGel (GFR)
Preparation of buffer, thawing tumor tissues, extraction of proteins
Dialysis, recovery and packing aseptic vials; Packing and shipping vials for customers
Cell Culture:
Maintaining cell lines for the production of microsome
Expression of proteins using baculovirus infected Hi5 cells and SF9 cells
Preparation of Supersomes prior to gamma irradiation
Preparation of UGT membrane prior to gamma irridation, Formulation
Quality Control:
HPLC, Lowry Protein Assay
Chlorzoxazone 6 hydroxylase Assay, Cytochrome P450
Uptake QC-scintillation (E3S, TEA, TCA, E217BG, PAH, GS)
Animals:
Liver prep from humans and animals, Frog care, Frog surgery and RNA injection
Administration:
Packing and shipping for customers, Contact customers regarding the products
Computer Skills:
Analyzing results from DNA sequencing using alignment tools
Searching promoter regions (both human and mice) for DNA methylation studies
Utilizing GraphPad Prism 4 for statistical analysis
Harvard School of Public School 01/2009-10/2012
Research Assistant
Research Assistant; part-time
Working under the supervisor of a postdoctoral scientist to run some independent experiments related to molecular biology, immunology, and cell culture
Cell Culture Techniques:
Transfection of 293T cells and transduction of shRNA using lentiviral vector related to survival from francisella infection; infection of RAW cells; differentiation of bone marrow derived dendritic cells (BMdDC); cell counts by trypan blue
Molecular Biology Techniques
Isolation of DNA/RNA from mouse spleen; Isolation of microRNA from mouse plasma
Isolation of plasmid DNA; restriction enzyme; PCR, and real time q-PCR; gel electrophoresis
Cloning and transformation in E.coli; bisulfite assay (DNA modification); protein assay (BSA)
Immunology Techniques
Isolation of CD4 T and CD11c+ from mouse spleen using microbeads
Verification of cell morphology by cytospin
Verification of cell purification by flow cytometry using expression markers MHC-II, CD11c+, CD11b+; ELISA for detection of IL8 and IL12, SDS-PAGE
Biochemics, MA 06/2011-1/2012
Research Associate
Assist the principal scientist to conduct the following assays with HPLC:
Solid phase extraction (SPE) of drugs including acetaminophen, gabapentin and celecoxib
Stability test of drugs: acetaminophen, moxonidine, benfotiamine, gabapentin, celecoxib
Franz Diffusion Cell – measuring concentration of sample applied on the pig skin
Horriba – measuring the particles size of drugs
Other responsibilities:
Maintain and trouble-shooting HPLC system; literature search of drugs prior to testing
Trouble shooting and procedure modifications for drugs tested
Calculate and prepare buffers/solutions; osmolarity test
Scion Pharmaceuticals, Inc, MA 11/2003-10/2005
Research associate
Survival and non-survival frog surgery; RNA injection, media prep and frog care
REFERENCES:
Available upon request
Contact this candidate