Dear Sir/Madam, As a professional biologist with a solid background inmanufacturing, quality control and laboratory settings, I am very interested inthe posted job and would like to apply please. I am a team player withextensive experience in research laboratories and I will be happy with theopportunity to become a member of your organization.

I look forward to the opportunity to meet with you tofurther discuss how my qualifications will benefit your organization. I can bereached by phone at 310-***-**** or by email at acps32@r.postjobfree.com. Following you can find my resume. Thank you for your consideration.Best regards Ava Masoumi

Ava Masoumi 5117 Otsego Court, Encino, CA 91436 acps32@r.postjobfree.com 310-***-**** SUMMARYBiology and biochemistry professional with a proven track recordof handling and working with human tissue, cells and specimens in a highlyregulated environment. Demonstratedability to work based on SOPs, GLPs and cGMPs. Extensive experience working in clean/sterile room as well as cold roomenvironments. Team player who hastrained students in bioscience research laboratories. Efficientand effective communicator. Known for collaborating well with a variety of groups,which led to publishedscientific manuscripts in high impact journals. Key strengths include: Advancedcell culture and cell banking Advanced molecular techniques Stem cellculture/ stem cell differentiation Drug screeningand drug development Plan, design and lead research experiments Quality/Environment/Health/Safety PROFESSIONALEXPERIENCEDavid Geffen School of Medicine at UCLA 2008 - 2014 Title: Researcher/Lab manager Certified inBiosafety, Radiation Safety, Bloodborne Pathogens, Chemical/BiologicalHazardous Waste ensuring all aspects of laboratory safety. Planned, designed,performed and led biochemical, immunological, microbiological, cellular,molecular experiments and a variety of microscopy in BSL2 and BSL2+ conditionsin a sterile biosafety cabinet while strictly adhering to OSHA laws and EHSregulations. Analyzed and recorded the results. Managed inventory of lab supplies and cell bank samples. Analyzed theresults and recorded the statistical data. Ensuredquality and audit readiness within stem cell lab, leading regular auditswithout any citations. Qualifiedtrainer in numerous bioscience labs (neurodegenerative diseases and regenerativemedicine), trained over 50 undergraduate students and technicians. Technical Universityof Braunschweig, Germany 2003 - 2007 Title: Research assistant in molecularbiology Cloned differentgenes using advanced molecular techniques, and cultured bacteria/cyanobacteriain bioreactors to produce recombinant proteins. Purified, solubilized, stabilized andconcentrated different proteins and components, and studied their biochemical characterization. Expert inprotein-protein interaction, and also protein precipitation with the followingmethods: ionic precipitation, pH, temperature and using organic solvents.

PROFESSIONAL DEVELOPMENT

BiotechBridge Training Academy Los Angeles ValleyCollege, 2015Selected to participate in a 6week technical training and workforce readiness program. Courses include: preparationfor workplace success. Blood Basics. Fractionation. Lean Manufacturing. CleanRoom Behavior. Aseptic Techniques. FDA Regulations. GMPs. GDPs. GCPs. CFR. FollowingSOPs. Validation. Attitude in Work. Biotech SkillsCertificate class- Environmental Monitoring/ Los Angeles Valley College, 2015Quality Control PRIOR RELEVANT EXPERIENCEUniversity of Medical Science – Iran 2001- 2003 Title : Lecturer/Food MicrobiologyLaboratory Manager Trained over 250 undergraduate students inbasic and food microbiology testing.Omid Wheat Flour Producer Co. Iran 2000 - 2001 Title : Licensed Technical Supervisor of Quality Control and Laboratory Supervised and evaluated routine activitiesof the laboratory resulting in the release of raw materials and products in atimely manner. Institute of Standard and Industrial Researchof Iran 1998- 2000 Title : Microbiology Laboratory Supervisor Audited products of a variety of food manufacturers to ensurequality standards. Certified in ISO 9000 and foodpackaging.

EDUCATION

Ph.D. in MolecularMicrobiology TechnicalUniversity of Braunschweig Braunschweig, Germany, 2007M.Sc. inMicrobiologyAzadUniversity Iran, 1999 B.Sc. in MicrobiologyTehran University Iran, 1995 TECHNOLOGY SKILLS Immunohistochemistry Immunocytochemistry Automation and high throughputscreening and reading 384 well plates by microplatereaders ELISA Light Microscopy by an inverted and conventionalmicroscope Confocal microscopy Fluorescencemicroscopy Mycoplasma detection in cell cultures Agarose gel electrophoresis SDS PAGE DNA isolation Plasmid transformation/ transfection Western blot Sterilization (autoclave, radiation,chemicals) Sequencing PCR FACS Cell line/Primary cell culture (human MSCs,human embryonic stem cells, human neural stem cells, Leukocytes, macrophages, motorneurons, endothelial, fibroblast, SY5Y, HEK, CHO, HUVEC and other cell lines) Cell based assays cell survival assays such as Trypan Blue,Propidium Iodide, MTT, Luciferase assay Producing iPS cells from human fibroblast cells,Embryonic stem cell differentiation to neurons and motor neurons Direct conversion of human fibroblasts toneural progenitor cells Adult stem cell differentiation to bone, muscle,adipo and cartilage cells Cellcounting by counting chambers Density gradient Immunomagnetic cell purification Testing BCA and Bradford protein assay UV absorption assays Endotoxin (LAL) testing Sterility testing Membrane filter technique Count Colony Forming Units (CFU) Water and food chemical pollution testing Bacterial and fungal staining Speed vacuum refrigeratedcentrifuge Affinitychromatography (biotin, GST or His tag, Protein A or G) & ion exchangechromatography (FPLC by AKTA) HPLC Immunoprecipitation Co-immunoprecipitation Effective drug dose assays Enzyme activity assay by spectroscopy Fluorescence stopped Flow Spectroscopy and radioactivityassay spectrophotometery Skilled in Microsoft Officesoftware (Word, Excel, PowerPoint), Photoshop, MS outlook and data analytic software suchas Graphpad Prism 6 LANGUAGESKILLS English, German and Farsi