Customer Service Assistant
Resume:
Kara Rogers
*** **** *** **, *******, CT *****
********@*****.***
EDUCATION
University of Connecticut
Master of Science in Ecology
2006
Concentration: Conservation Biology and Biodiversity
Relevant courses: Toxicological pathology, Histology, Principles of Pathobiology, Clinical Chemistry, Virology, Immunobiology, Design and analysis of agricultural experiments, Conservation biology, Fundamentals of GIS, Environmental Law, Evolutionary Biology, Ornithology lab, Organisms and ecosystems.
University of Connecticut
Bachelor of Science in Biology and Environmental Science
2001
Relevant courses: Biology, Environmental health, Organic chemistry, Limnology, Meteorology, Oceanography, Environmental planning and management, Ecology, Methods of Ecology, Earth surface processes, Geology, Environmental resource economics, Systematic botany, Genetics, Animal Physiology, Ornithology, Parasitology.
TEACHING EXPERIENCE
Teaching Assistant – Introduction to Biology, University of Connecticut
Sept. 2005-
July 2006
Led four lab sections a week for three semesters in introductory biology for undergraduate students. Duties include, lecturing, creating quizzes, grading, supervising student use of equipment and chemicals, collaborating with instructors and other TAs on laboratory curriculum issues, and explaining and answering questions regarding biological concepts to students with little to no background in biology.
WORK/RESEARCH EXPERIENCE
University of Connecticut Department of Pathobiology and Veterinary Science Sept 2008-Present
Research Assistant II
Work in a molecular biology lab investigating the genetic determinants of viral pathogenicity focusing on protein expression and the effect on virulence, antigenicity and immunogenicity.
- Protein expression (including recombinant proteins), detection and purification using E. coli and baculovirus systems.
- Protein-protein interaction using the yeast two hybrid system
- Skilled in performing and analyzing various molecular techniques (e.g. PCR, cloning, mutagenesis, cell culture)
- Collaborated with and performed selected assays at the Plum Island Animal Disease Center (ARS/USDA)
- Skilled in gene expression study techniques (e.g RNA extraction, purification and quantity/quality assessment as well as real-time qpcr)
Work focused on various virology techniques through vaccinia virus studies (e.g. mouse tissue collection, establishing and maintaining primary cell lines from tissues, cell infection studies, qpcr to assess viral load, maintaining and genotyping mouse colonies)
Work in an immunology based lab examining the effects of sickle cell disease on immunology and infectious disease. Duties include maintaining, breeding and genotyping a mouse colony, collecting mouse tissues and creating single cell suspensions, performing and analyzing ELISA assays, growing bacterial cultures for infection studies.
- Manages routine lab functions and trains new undergraduate and graduate students
University of Connecticut Department of Physiology and Neurobiology Dec. 2007- Sept 2008
University of Connecticut Department of Pathobiology and Veterinary Science May 2008-Aug 2008
Research Assistant II
Worked as a research assistant in a physiology/neurobiology lab focusing on
changes in neuronal function as it relates to changes in auditory pathways.
Duties include: Perfusion of rats and tissue collection, tissue sectioning on both vibratome and freezing microtome, Immunohistochemistry (DAB and Fluorescent), western blot, preparing tissues for electron microscopy, analysis and digital image documentation of receptor activity in specific areas of the brain using a light microscope, preparing solutions, maintaining equipment, and ordering supplies.
Additional temporary work in a pathobiology lab focusing on changes in immune function as a result of environmental contaminants. Duties include, performing assays to study changes in immune function, specifically lymphocyte proliferation, phagocytosis and respiratory burst, of marine mammals, mice and humans. Also, gained experience in ELISAs, flow cytometry and data analysis.
Tufts University, Cummings school of Veterinary Medicine
Research Technician
Worked as a research technician in a Neuroscience/Maternal behavior lab.
Duties included:
Working with rats for the purpose of maternal behavior studies involving mating, weighing, drug injections, performing behavioral observations, perfusions and decapitations for the purpose of preserving brain tissue.
Other laboratory duties included standard lab practices such as preparing solutions, maintaining equipment, ordering supplies. Performed Immunohistochemistry on free floating tissue sections and visualized slides using a fluorescent microscope. Maintained various data spreadsheets.
April 2007 –
July 2007
Connecticut Department of Environmental Protection
Intern
Worked on several project with the Wildlife Division:
Survey of Raptor Species in CT. Duties included; working independently to establish survey routes using GPS equipment in 5 areas based on predetermined criteria, identifying a variety of raptor species based on sight and sound, and maintained data sheets.
Survey of Owl species in CT. Duties included; working independently to identify owl species by sight and sound in established routes and maintain data sheets.
Water shrew study. Worked on a study to document the presence and microhabitat associations of water shrews at Whitehall Conservation center in Litchfield, CT. Duties included; working independently to set up “traps” for fecal collection in various microhabitat types, collection and some identification of fecal samples, classifying microhabitat type and possible associations, geo-referencing sample points.
Feb. 2006 – July2006
University of Connecticut, Department of Pathobiology and Veterinary Science
Graduate Research Assistant
Participated in several research and wildlife disease surveillance projects:
West Nile virus surveillance for Connecticut and Vermont. Duties included; avian necropsy for the purpose of removing brain sample for diagnostic testing, performing diagnostic tests using RT-PCR (including real time) and gel electrophoresis, and analysis and reporting of results.
Chronic Wasting disease surveillance. Trimmed suspect tissue samples for histological preparation.
Cetacean poxvirus study. Worked through UCONN at the Plum Island Animal Disease Center (USDA) in an effort to sequence cetacean and pinniped poxviruses. Performed techniques in virus isolation and electron microscopy. Obtained federal security clearance.
Raccoon Zoonosis study. Designed a study to test for the presence of several zoonotic diseases in raccoons in CT, including West Nile virus, Eastern Equine encephalitis virus, giardia, brucella, toxoplasma, raccoon roundworm and salmonella. Duties included; establishing a protocol for obtaining blood, urine and fecal samples, establishing diagnostic testing protocols that included ELISAs, bacterial culture, and agglutination tests.
Sept. 2002 –
May 2005
Mystic Aquarium
Volunteer
Volunteer in husbandry of pinnipeds and penguins. Duties include; cleaning habitats, preparing food, and feeding. Worked with Northern fur seals, Stellar sea lions and African penguins.
May 2001-
Aug. 2002
NewAlliance Bank
Teller
Duties include, Customer service and problem solving, computer skills (troubleshooting new systems), money handling.
Dec. 1998 –
Sept. 2005
PUBLICATIONS
Gavrilov BK, Rogers K, Fernandez-Sainz IJ, Holinka LG, Borca MV, Risatti GR. (2011). Effects of glycosylation on antigenicity and immunogenicity of classical swine fever virus envelope proteins. Virology. 2011 Nov 25;420(2):135-45.
Gladue DP, Gavrilov BK, Holinka LG, Fernandez-Sainz IJ, Vepkhvadze NG, Rogers K, O'Donnell V, Risatti GR, Borca MV.(2011). Identification of an NTPase motif in classical swine fever virus NS4B protein. Virology. 2011 Mar 1;411(1):41-9
Gladue DP, Holinka LG, Fernandez-Sainz IJ, Prarat MV, O'Donell V, Vepkhvadze N, Lu Z, Rogers K, Risatti GR, Borca MV.(2010). Effects of the interactions of classical swine fever virus Core protein with proteins of the SUMOylation pathway on virulence in swine. Virology. 2010 Nov 10;407(1):129-36.
Bogomolini AL, Frasca S Jr, Matassa KA, Neilsen O, Rogers K, DeGuise S. (2015). Development of a duplex real-time RT-qPCR for the quantification of phocine distemper virus. Journal of Wildlife Diseases. Apr 51(2):454-65.
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