Maintenance Lab Technician

KRSNA VIEIRA

*** ******* ******* ************, ** 20877 240-***-**** ***********@*****.***

OBJECTIVE

To obtain employment in the biomedical research industry in an innovative and collaborative environment that will also allow for both professional and personal advancement.

EDUCATION

Bachelor of Science, Biological Sciences

University of Maryland, Baltimore County, Baltimore, MD

May 2015

RELEVANT EDUCATION/TRAINING

Certificate of Completion, Biotechnology

FAES Junior Scientist Training Program, National Institutes of Health, Bethesda, MD

May 2012

9-month in-lab workshop covering a wide range of laboratory techniques in molecular biology, including, but not limited to, cloning/library construction, DNA microarrays, In Situ hybridization, Real-Time/ qPCR, and flow cytometry.

EXPERIENCE

O.R.I.S.E Undergraduate Internship

Food & Drug Administration, Division of Cellular and Gene Therapies, TVBB-Bethesda, MD

Full-time summer employment. Part-time semester employment

August 2010 to November 2013

Maintenance and calibration of Tempo HPLC/MALDI target plate deposition hybrid instrument for MALDI TOF/TOF analysis.

Participated in analytical protein methodologies-Protein and peptide characterization using reverse-phase HPLC and Mass spectrometry (nano-LC, MALDI-TOF/TOF).

Maintenance of mammalian cell lines and aseptic technique

Conducted laboratory techniques for quantitative and qualitative purposes including, Western Blots, ELISA, PCR, gel-free fractionation, hydrostatic protein extraction, hemagglutination inhibition assays.

Effective collaboration with branch postdoc fellows in their research projects: Qualitative analysis of CYPs expression, proteome analysis of hBMSCs, proteomic profiling of multipotent stem cells.

Undergraduate Intern/Lab Technician

Food & Drug Administration, Division of Cellular and Gene Therapies, TVBB-Bethesda, MD

June 2008 to August 2010

Laboratory Maintenance and supply inventory

Maintaining an organized laboratory journal

Media and solution preparation

Sample preparation via SDS-page, enzymatic digestion, gel-free electrophoresis for Mass Spectrometry analysis.

SKILLS & ABILITIES

Detail-oriented with good organizational skills and record keeping.

Proficiency with word processing and spreadsheets.

Demonstrated ability to work in collaborative research team settings and learn new technologies.

Successfully multi-task technical laboratory techniques.

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