*****************@*****.*** ALFONSO F. DE LEON 201-***-****
EDUCATION & HONORS
Fairleigh Dickinson University, Teaneck, NJ Read, write, speak, interpret and translate
Spanish
Masters of Science: Biology/Chemistry, May 2013; GPA 3.84 MS Word, Excel, Power Point,
Outlook
Bachelors of Science: Biology; Minor: Chemistry, May 2012
Dean’s list, four years Chi Alpha Epsilon Phi Zeta Kappa Phi Omega Epsilon Sigma Lambda Beta
Selected Order of Omega’s Fraternity Member with the highest Grade Point Average on May 2013
QUALIFICATIONS
Bacterial and Human cell culturing and testing techniques such as Plate Count, Pour Plate, Streaking, Isolation, ID,
Celsis, Gram Staining, Membrane Filtration, Microbial Limits, Bioburden, LAL, Trypsinization; Dosing/Dilutions;
SDS PAGE; PCRs; Microarrays; ELISA; Northern, Southern, and Western Blotting; Isolation, Purification of Genetic
Material; SOPs; USP; GMP/GLP; Micro pipetting; Microinjections; Agarose Gel Electrophoresis; Restriction mapping;
Chromatography; Radioactive labeling; Fluent with Chemicals; Titrations; HPLC; GC; IR, UV Vis Spectroscopy;
Viscosity; Osmolarity; Ph
PROFESSIONAL SCIENTIFIC EXPERIENCE
Colgate Palmolive Inc., Morristown, NJ July 2014 Present
Contract Scientist
• Supervise and manage a team of 4 by delegating tasks and ensuring their prompt completion on a daily basis
• Implement recording, reviewing and reporting of controlled document data; sample receipt, login and processing
• Perform daily inspection of laboratory, 7 incubators, 5 refrigerators, 3 biosafety cabinets, 2 water baths, 2 autoclaves
• Prepare, organize and qualify media for testing (MLA, SDA, SDBT, R2A, MacConkey agar, TAT broth, Tween)
• Perform collection/testing of 70 water samples, 30 raw materials, 160 finished products within an hour limit each
• Execute environmental monitoring (airs and contact) of testing, manufacturing facilities and C&S validation swabs
Gibraltar Laboratories Inc., Fairfield, NJ October 2013 – July 2014
Senior Microbiologist & Calibration Specialist
• Supervised, Trained and Provided proficiency testing of Technicians; Aided in Audits, CAPA, LIRs; Developed
SOPs
• Performed routine inspection, IQ/OQ/PQ, calibration, certification, validation and temperature verification of 35
incubators, 4 freezers, 3 laminar flow hoods, 4 biosafety cabinets, and other analytical instruments
• Performed Sterility testing, Class 10/BSL3; Test/C&S Validations, Bioburden and LAL testing; Maintained cultures
• Performed cell culture and staining using simple and differential staining techniques such as Gram Stain, Acid Fast
stain, Eosin, Basic, Fixation, Mounting, Permeabilization, as well as organelles staining dyes
Fairleigh Dickinson University, Teaneck, NJ September 2010 – October 2013
Tutor
• Provided academic support to students in Biology, Chemistry, College Algebra, Pre calculus, University Physics,
Biochemistry, Microbiology, Genetics, Cell & Molecular Biology, Physical Organic Chemistry, Statistics
National Career Institute, East Orange, NJ June 2013 – October 2013
Instructor
Ensured students’ complete understanding of material using power point presentations, lectures, learning exercises,
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practicals, and various evaluating measures. Provided students with proper guidance in their possible career paths
Albert Einstein College of Medicine, Bronx, NY May 2012 – August 2012
Contract Research Intern
Characterization of Histidine Residues Responsible for pH Sensitivity in Purine Uptake of Plasmodium vivax
Equilibrative Nucleoside Transporter 4
• Performed bacterial transformations using linearized DNA to express and extract mRNA from bacteria
• Injected mRNA in oocytes using microinjector and used radioactive labeling to track protein expression
• Presented work in various scientific symposiums. Recognized as a leader and research specialist
LABORATORY INSTRUMENT/EQUIPMENT EXPERTISE
Micropipettes; Biological Safety Cabinets; Laminar Flow Hoods; Tuttnauer/Getinge Autoclaves; Ph Meters; Centrifuges;
Incubators/Shakers; Refrigerators; Compound, Fluorescence and Light Microscopes; Micro Osmometer; Hot/Stir plates;
Analytical Scales; SAS and Biotest Hycon RCS Air Samplers; Met One Large Particle Counter; Melting Point
Apparatus; Water Activity Meter; Lazer Diffraction Particle Size Analyzer; IR and UV Vis Spectrometer; HPLC; GC;
Thermocouples; Gruenberg Industrial Depyrogenation Ovens; Colony Counter; Luminometer; Vortex Mixers; Water
Baths; Viscometer
LEADERSHIP and SUPERVISORY EXPERIENCE
Fairleigh Dickinson University, Teaneck, NJ August 2012 – August 2013
Resident Assistant
• Managed various administrative duties including maintenance requests, incident reports, etc
• Enforced and upheld university policies and acted as a liaison between residents and Department of Residence Life
• Counseled peers on personal, academic, and career concerns
• Created, planned, and implemented educational, social, and recreational programs
Vector Marketing, Wayne, NJ May 2011 – April 2012
Branch Manager & Customer Care Specialist
• Managed and conducted a sales and receptionist team of 20; interviewed, recruited and trained sales representatives
• Organized and ran staff meetings twice a week; attended bi monthly leadership building business conferences
• Researched products and market; received and verified inventory and entered in stock system
• Acknowledged as business expansion expert and certified field trainer
• Received extensive Branch Manager Training as well as Several Performance, Training, and Development Awards
The Children’s Place, Passaic, NJ July 2010 – September 2011
Lead Customer Service Specialist
• Maintained appropriate stock levels following company standards of merchandise presentation, signage and display
• Aware of customer activity and responded with a sense of urgency, prioritizing assisting customers over other tasks
• Maintained a clean, organized and efficient stockroom while adhering to standards and safety requirements
• Guaranteed company assets by ensuring adherence to all Loss Prevention procedures
• Proactively contributed to creating a team atmosphere that is flexible and enjoyable
Fairleigh Dickinson University, Teaneck, NJ November 2008 – December 2009
Hall Security Assistant
• Enforced and upheld university policies. Maintained knowledge of all emergency policies and procedures
• Assessed calmly and responded promptly to disciplinary problems involving residents and staff members.
• Maintained documentation of all incident reports and presented to supervisors
• Established good communications and relationships with residents, supervisors and other staff
GRADUATE RESEARCH EXPOSURE
Fairleigh Dickinson University, under Dr. Alice Benzecry, PhD and Dr. Neena Philips, PhD
Fall 2012 – Spring2013: Antimicrobial Activity of Chitosan Nanocomposites Enhanced with Hops Plant Extracts Tested
in Solution as well as Skincare Formulations Against Pseudomonas, Staphylococcus, and Candida species
• Formulated nanocomposites and creams enhanced with different plant extracts (Xanthohumol and Lupulone)
• Prepared enriched media (broth and agar) to keep bacterial cultures
• Tested these nanocomposites in solution and creams using various assays and standard sterilization procedures
• Kept and collected all data in a laboratory notebook; discarded all hazardous materials using EPA standards
• Presented poster at the Hudson Bergen Chemical Society’s annual symposium
• Presented poster at the New York Society of Cosmetic Chemist; 3rd place poster prize
• Presented orally at the New Jersey Academy of Science’s annual meeting
Fall 2011 – Spring 2012: Low [H2O2] Effectively Promote Angiogenesis through VEGF Expression in Human Fibroblast
Cells without Cell Loss, Toxicity or Oxidative Stress, Proving Beneficial for Tissue Repair and Wound Healing
• Dosed human fibroblast with 0.1, 1.0, 10 µ M concentration of H2O2 using micro pipettes
• Tested for cell proliferation, apoptosis, membrane damage, VEGF expression, protein content, and antioxidant
activity using various molecular techniques
• Presented poster at the Hudson Bergen Chemical Society’s annual symposium
EXTRACURRICULAR
Sigma Lambda Beta International Fraternity Incorporated
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Director of Campus Relations Director of Philanthropy/Continuing Services Secretary STAMPEDE step team
Fairleigh Dickinson University
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Secretary of Green Team Member of Pri’sizhen step team