Data Entry Maintenance
Resume:
Resume of William Joseph Tighe
***********@*****.***
RELEVANT SKILLS:
. Tissue/Cell Culture, ELISA's/Assay's
. Protein Purification and Characterization (Liquid Chromatography)
. SDS-PAGE and IEF
. Monoclonal Antibody Production/Fusions
. Lyophilization
. Animal Observation
. In-vivo Animal Surgical Procedures
. Titration
. UV-VIS
. Spectroscopy
. Aseptic Technique
. Clean Room Behavior
. Lab Notebook Recording
. Data Entry
. GSOP
. cGMP
. Communication
Between and Training Team Members
. Cell Line Maintenance
. Cell Banking
RELEVANT EXPERIENCE:
Genzyme Biosurgery
Cambridge, MA
Manufacturing Associate
Mar, 2011-May, 2014
. Receive patient biopsies and digest them with enzymes to extract
chondrocytes for an approved FDA product.
. Scale up extracted cell patient lines for later retransplant back
into the patient as needed.
. Worked in a class 10,000 room.
. Performed final product release of cells.
. Performed batch record reviews, as well as input for SOP's changes
. Notify the people responsible for ordering lab supplies when stocks
ran low
. Cryopreservation of individual patient cell lines, as well as
putting the cells location in the database
. Worked closely with patient coordinators to ensure smooth
transition of cell lines to hospitals
. Aseptic Technique-We receive autologous chondrocites from patients
to be called up for retransplant into the patient. Aseptic
technique was imperative.
. Al of the above mentioned work was carried on in a 10,000 clean
room under cGMP conditions
Siemens Healthcare Diagnostics Oncogene Science
Cambridge, MA
Biochemist I January,
2001-September, 2010
. Develop new and innovative biomarkers to aid in cancer research along
with aiding in pharmaceutical collaborations.
. Performed tissue/cell culture on monoclonal antibodies/hybridoma cell
lines
. Concentrated on monoclonal antibody production (hybridoma) utilizing
fusions, sub cloning, scale up in cell culture, screening assays,
protein purification, protein characterization, and reagent
preparation for all aspects through in house phase 1 approval.
. Fusion of mouse spleen cells with human immortalized cell line SP2/O's
. Protein isolation using limited dilution sub cloning and liquid
chromotophy
. Vast expireance in running ELSA,s and assays, as well as creating
monoclonal antibodies, and building ELISA kits
. Vast experience with Gel electrophoresis
Army National Guard
Army National Guard Flight/Combat Medic
1992-February, 2010
. Army National Guard Flight/Combat Medic, 2004 Baghdad/Mosul, Iraq:
Iraq Survey Group worked with multinational teams in search of Weapons
of Mass Destruction, as team medic.
. Kurdistan, Iraq: Ran clinic from diagnosing, treating, and prescribing
medications to the locals as well as treating trauma patients. Train
Iraqi Police and soldiers in Combat Lifesaving Course techniques.
Clean Harbors
Environmental Chemist, Environmental Services
1997-2001
. Provided identification, of client's waste streams for the proper
disposal under RCRA, and also acted as a first responder to HazMat
incidents for the state of New Hampshire.
EDUCATION:
Excelsior College
Bachelors of Science in Liberal Arts with a Science Concentration
Anticipated completion date Sep, 2015
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