Assistant Manager

Yulan Ai

Curriculum Vitae

Address: *** ***** ******, *******, ** 02478

E-mail: *******@*****.***

Cell phone: 617-***-****

Education

****-**** ************ ***********, ******* Academy of Medical Sciences, Nanjing,China

1987-1992 B.S. Xinxiang Medical School, Xinxiang, Henan, China

Statement of personal objective

Applicant desires a position of Research Assistant

Professional and Research experience

2012-present: Research Assistant at the Immunobiology Department, Joslin Diabetes

Center, Boston

• Generation of expression vectors

• Expression, purification, and analysis of mammalian proteins

• Encapsulation of proteins into nanoparticles to be delivered to animals

• Mammalian cell culture

• Flow cytometry on in vitro grown cells and blood samples

2008- 2012: Research Assistant and Lab manager at the Islet Cell and Regenerative

Biology Department, Joslin Diabetes Center, Boston

• Management of general lab supplies and reagents

• Maintenance of mammalian cell lines

• DNA transfection into mammalian cells

• Promoter luciferase assay

• Protein quantification assay

• DNA plasmid cloning

• Bacterial transformation

• Plasmid DNA preparation

• PCR, quantitative real-time RT-PCR

• DNA and RNA isolation

• Maintenance and genotyping of mice

• Measurement of mouse blood glucose level

• Animal dissection

• Cryosectioning

• Immunohistochemistry on tissue sections

• Imaging microscopy

2004-2005: Research Assistant at Massachusetts General Hospital, Boston

• Sectioning of human tissues

• Immunohistochemistry on tissue sections

2004-2005: Research Assistant at Joslin Diabetes Center, Boston

• Generation of cell culture media, buffers, and agar plates for Joslin Research Section

2003-2004: Volunteer at Massachusetts General Hospital, Charlestown

• Study on animal behaviors

• Biopsies on animals

• Tissue sectioning

• Immunohistochemistry on tissue sections

1992-2003: Lab Manager at The First Affiliated Hospital of Xinxiang Medical School,

China

• Diagnosis and treatment of patients

• Examination of patients’ histological samples

• Examination and identification of patients’ culture samples

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