lab technician

Archana V. Pai

** ********** ***, **** ****** NJ

Phone: 908-***-**** email: *********@*****.***

PROFESSIONAL SKILLS

Molecular Biology: Cell culture, Transfection, Screening cells for antibody

production, Single cell cloning, ELISA and HTRF, DNA and RNA Purification,

Nucleic acid qualitation using Agilent, Microarray, Real time PCR, Southern

blotting, Cloning, Restriction digest analysis, RT-PCR, in situ

Hybridization, Northern blotting, Drug permeability & solubility assay,

Apoptosis assay.

Computer Skills: MS Office (Word, Excel, PowerPoint), Minitab, SoftMax Pro,

Graph Pad Prism

EDUCATION

Master of Science: Biotechnology, William Paterson University, NJ

Master of Science: Microbiology, Mangalore University, India.

Bachelor of Science: Mangalore University, India.

PROFESSIONAL EXPERIENCE

Medarex, Bloomsbury NJ 09/06-

04/08

Assistant Scientist II: Transfectoma: Cell Line Development

Conduct serum free CHO cell culture work, transfection, cell line screening

and selection, single cell cloning and performing assays such as

ELISA and HTRF.

. Transfection of BioWa pIE cells using electroporation method

. Screening cells for antibody producing cells using HTRF or

ELISA and expansion of cells from 96 well plate to shake flask

. Single cell cloning and screening monoclonal cells for antibody

production

. Construct cell banks.

. Worked with GS expression system projects when needed

Johnson & Johnson, Veridex LLC, Warren NJ 06/04-

06/06

Scientist I: Assay Development

Assist in development of nucleic acid based assays for cancer diagnostic

products using Real Time PCR and Microarray techniques.

. Breast Lymph Node Assay : experimentation to support Phase 3

stage, Panel Production, Validation testing, Ancillary Studies

. Thyroid Assay And Colon Assay: experiments to optimize RNA

isolation, amplification, labeling, and hybridization protocols using

Affymatrix Gene Chip

Hoffmann-La Roche Inc., Nutley NJ 06/ 02 -

06/04

Laboratory Assistant: Drug Discovery R&D

. Maintain MDCKII and Caco-2 cell lines and seed cells. Perform

uni- and bidirectional drug permeability assays using MDCKII and Caco-

2 cells.

. Perform high throughput drug solubility test: THESA

. Worked with liquid handler- TECAN

. Experience with Guava cell counter

Summer Research: William Paterson University, NJ

05/01- 12/01

Cloning of cannabinoid receptor in Zebra fish- experience in primer

designing, RT-PCR, 3'-RACE, Southern Blotting.

Teaching Assistant, William Paterson University, NJ 09/00

- 05/01

Conducted field biology laboratory classes and field trips

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