Formulation Scientist

Prabagar Balakrishnan

** ***** ***** ****, ******, CO 80012 / Tel: +1-720-***-****

*********@*****.***

Education

Ph.D. in Pharmaceutical Science, 2006 - 2009

Yeungnam University, Department of Pharmaceutics, College of Pharmacy, with GPA 4.33/4.5,

Republic of Korea

M.S. in Pharmaceutical Science, 2004 - 2006

Yeungnam University, Department of Pharmaceutics, College of Pharmacy, with GPA 4.35/4.5,

Republic of Korea

Bachelor's degree in Pharmacy, 1995 - 1999

The Tamilnadu Dr. M. G. R Medical University, India

Experience

Research Professor - Institute of Pharmaceutical Science and Technology, Research institute,

(10/ 2012-03/ 2014) Hanyang University in South Korea.

Developed, optimized and characterized Hyaluronic acid-ceramide (HACE) and

Cyclodextrin based protein micro-particles by lyophilization and electro spray drying for

enhanced bioavailability and stability.

Designed, conducted and interpreted novel formulations and process development

experiments for poorly water soluble drugs, troubleshoot and supervised six researchers

in the following research projects

- Comparison of a solid SMEDDS and solid dispersion for enhanced stability and

bioavailability of clopidogrel napadisilate

- Physicochemical characterization, in vitro and in vivo assessment of spray dried

microencapsulated, solid dispersion and Inclusion co mplexation of fenofibrate for

enhanced dissolution and bioavailability.

- Solid Dispersion of docetaxel by electro spray drying and spray drying methods.

- Self-emulsifying drug delivery system for clopidogrel napadisilate monohydrate

assessment of stability and bioavailability.

- Thermo sensitive liquid suppository containing solid lipid nanoparticles for the

controlled delivery of doxorubicine hydrochloride for colon cancer

- Docetaxel nanoparticles for tumor targeting

Estabilished cell culture based works and related techniques in the lab

Prabagar Balakrishnan - Resume

Scientific writing for publications and technical writing for grant proposals.

Senior Scientist - Utah-Inha DDS & Advanced Therapeutics Research Center, South Korea.

(10/2011- 09/2012)

Developed and characterized monodispersed Doxorubicin loaded microspheres using

human serum albumin and dextran (160 to 300 m) for transcatheter arterial

chemoembolization treatment in hepatic cancer.

Developed and optimized polymeric Nanocapsule containing SEDDS oil core for the

controlled and enhanced bioavailability of cyclosporine

Developed PLGA nanoparticles for sustained release of small molecules

Attended technical meetings with other disciplinary scientist and conducted scientific

briefings, as required.

Postdoctoral Researcher - Seoul National University, South Korea.

(09/2009- 10/2011)

Developed of MPEG–PLA/Pluronic® copolymer micelles for enhanced bioavailability

and multidrug resistance of docetaxel.

Prepared and optimized docetaxel solid lipid nanoparticles containing sphingolipid and

Pluronic® polymer for enhanced bioavailability and to overcome Multi drug resistance.

Developed polysaccharides-based protein microparticles.

Developed Poloxamer and carbopol based hydrogel formulation for the nasal delivery o f

Fexofenadine Hydrochloride.

Troubleshoot and provided technical assistance, and worked with graduate researchers in

various formulation projects.

- Cross-linked hyaluronic acid-based flexible cell delivery system: Application for

chondrogenic differentiation

- A novel vesicular carrier, transethosome, for enhanced skin delivery of

voriconazole: Characterization and in vitro/in vivo evaluation

- Evaluation of protein stability and in vitro permeation of lyophilized

polysaccharides-based microparticles for intranasal protein delivery

- Preparation and evaluation of fexofenadine microemulsion for intranasal delivery

Scientific writing for publications and technical reports for grants.

Research Assistant - Department of Pharmaceutics, Yeungnam University, South Korea.

(03/2004- 8/2009)

The effect of -cyclodextrin complexation on the solubility, dissolution, bioavailability

and hepatotoxicity of clotrimazole.

Prepared and characterized BrijTM or SpanTM based minoxidil niosome for enhanced skin

permeation.

Prabagar Balakrishnan - Resume

Developed Solid Self emulsifying drug delivery system (SEDDS) using dexibuprofen as

model drug for increased oral bioavailability.

Developed liquid SEDDS for coenzymeQ10 for enhanced solubility and bioavailability.

Prepared manuscripts for publications, worked in and learned several other formulation

techniques such as solid dispersion of sibutramine, Itraconazole and poloxamer based

piroxicam hydrogel, microemulsion and salicylic acid ester prodrug for UV protection.

Production Planning Coordinator - Epoch Pharmaceuticals, Chennai, India,

(01/2001 - 07/2003)

Pharmacist - Vijay Pharmacy, Chennai, India,

(06/1999 - 12/2000)

Research interests: Increasing solubility, dissolution and bioavailability of poorly water soluble

drugs. Immediate and controlled release delivery of small molecules and proteins. Parenteral,

oral, topical and nasal delivery systems. Nanoparticles, Nanocapsule technologies for targeted

delivery. Biomaterials. Oral Lipid Based Formulations. liposome and niosome formulations.

Technical Skills

Over nine years of laboratory and research experience in the pharmaceutical science field

Animal Handling - Rat, rabbit, and mice

Tumor drug targeting - Flowcytometry and analysis of cell populations, primary and

cancer cell culture, cytotoxicity screening (MTT, MTS and SRB), apoptosis assays,

histological and biochemical assessment of tumor remission in animal models (Ant itumor

study).

Pharmaceutical dosage formulations - Nanocapsules, Nanoparticles and polymeric

micelles, liquid oral dosage forms, Microspheres, Microparticles, Microemulsions,

Multiparticulate controlled release formulations, Scaffolds, Self-Emulsifying Drug

Delivery Systems (SEDDS), Solid SEDDS, Suppository, Solid Dispersions.

Thermoreversive Hydrogels, Inclusion Complexation, liposomes, Niosomes, Tablets and

capsules.

Prabagar Balakrishnan - Resume

Dosage form characterization - Dissolution, Caco-2 cell permeability assay,

disintegration, friability and hardness testing for tablets, flow ability, viscosity, particle

size characterization (DLS), SEM and TEM. Data analysis for pharmacokinetics, and

bioequivalence studies. Skin permeability and flux studies.

Analytical techniques - HPLC, LC MS-MS, TGA, UV/Vis and Fluorescence

spectroscopy, Differential Scanning Calorimetry (DSC), X-ray powder diffraction, FTIR

spectroscopy.

Protein work - Electro spray drying, protein purification, SDS-PAGE, BCA Assay

Statistics and software - Proficient in Microsoft Office suite, Endnote, Sigma Plot,

SPSS.

Personal : Ability to learn new techniques, work methodically, efficiently and

accurately, ability to work in a multidisciplinary group and independently; excellent

written and verbal communication.

Publications

International peer reviewed SCI papers

1. Prabagar Balakrishnan*, Chung Kil Song and Han Gon-Choi. Docetaxel nanoparticles

containing sphingolipid and Pluronic® polymer for enhanced bioavailability and

overcoming MDR. ( Under review International journal of pharmaceutics)

2. Prabagar Balakrishnan*, Chung Kil Song and Han Gon-Choi. Carbopol polymer based

hydrogel for enhanced bioavailability of fexofenadine by nasal delivery. (Archives

Pharmacal research)

3. Jahn, A. Song CK, Balakrishnan P, Hong SS, Lee JH, Chung SJ, Kim DD (2014) AAPE

proliposomes for topical atopic dermatitis treatment. J Microencapsul.

doi:10.3109/02652048.2014.932027

4. Kim DW, Kwon MS, Yousaf AM, Balakrishnan P, Park JH, Kim DS, Lee BJ, Park YJ,

Yong CS, Kim JO, Choi HG (2014) Comparison of a solid SMEDDS and solid

dispersion for enhanced stability and bioavailability of clopidogrel napadisilate.

Carbohydrate Polymers 114 (2014) 365–374.

Prabagar Balakrishnan - Resume

5. Doh HJ, Jung Y, Balakrishnan P, Cho HJ, Kim DD (2013) A novel lipid nanoemulsion

system for improved permeation of granisetron. Colloids Surf B Biointerfaces. 1;101:475-

80.

6. Park M*, Balakrishnan P* (*Equally Contributed), Yang SG (2013) Polymeric

nanocapsules with the SMEDDS-core for the controlled and enhanced oral absorption of

cyclosporine. Int J Pharm30; 441(1-2):757-64.

7. Balakrishnan P, Song CK, Kim DD, Yong CS, Choi HG (2012) Inclusion complex

effect on the bioavailability of clotrimazole from poloxamer based solid suppository.

Arch Pharm Res Vol 35, No 7, 1169-1175, 2012.

8. Song CK, Balakrishnan P, Shim CK, Chung S-J, Kim DD (2012) A novel vesicular

carrier “Transethosome” for enhanced skin delivery: characterization and in vitro

permeation studies.Colloids and Surfaces B: Biointerfaces 1;92:299-304.

9. Hwang HD, Cho HJ, Balakrishnan P, Chung CW, Yoon IS, Oh YK, Byun Y, Kim DD

(2012) Cross-linked hyaluronic acid-based flexible cell delivery system: application for

chondrogenic differentiation. Colloids and Surfaces B: Biointerfaces.91, 1, 106-113.

10. Song CK, Balakrishnan P, Shim CK, Chung S-J, Kim DD (2011) Enhanced in vitro

cellular uptake of P-gp substrate by Poloxamer-modified liposomes (PMLs) in MDR

cancer cells. J Microencapsul 28: 575-581.

11. Chung CW, Kang JY, Yoon IS, Whang HD, Balakrishnan P, Chung KD, and Kim DD

(2011) Interpenetrating polymer network (IPN) scaffolds of sodium hyaluronate and

sodium alginate for cartilage tissue engineering. Colloids Surf B Biointerfaces 88: 711-

716.

12. Cho HJ, Balakrishnan P, Chung SJ, Shim CK, Kim DD (2011) Evaluation of protein

stability and in vitro permeation of lyophilized polysaccharides-basedmicroparticles for

intranasal protein delivery. Int J Pharm416: 77– 84.

13. Shanmugam S, Baskaran R, Balakrishnan P, Thapa P, Yong CS, Yoo BK (2011) Solid

Self-Nanoemulsifying Drug Delivery System (S-SNEDDS) Containing

Phosphatidylcholine for Enhanced Bioavailability of Highly Lipophilic Bioactive

Carotenoid Lutein. Eur J Pharm Biopharm 79: 250-257.

14. Im JS, Balakrishnan P, Oh DH, Kim JS, Jeon EM, Kim DD, Young CS, Choi HG (2011)

Evaluation of salicylic acid fatty ester prodrugs for UV protection. Drug DevInd Pharm

Prabagar Balakrishnan - Resume

37: 841-848.

15. Oh DH, Balakrishnan P, Yong CS, Choi HG (2011) Effect of process parameters on

nanoemulsion droplet size and distribution in SPG membrane emulsification. Int J Pharm

14:191-1977.

16. Cho HJ, Balakrishnan P, Park EK, Song KW, Hong SS, Jang TY, Kim KS, Chung SJ,

Shim CK, Kim DD (2011) Poloxamer/cyclodextrin/chitosan-based thermoreversible gel

for intranasal delivery of fexofenadine hydrochloride. J Pharm Sci 100: 681-691.

17. Park YJ, Xuan JJ, Oh DH, Balakrishnan P, Yang HJ, Yeo WH, Lee MK, Yong CS,

Choi HG (2010) Development of novel itraconazole-loaded solid dispersion without

crystalline change with improved bioavailability. Arch Pharm Res 33: 1217-1225.

18. Cho HJ, Balakrishnan P, Shim WS, Chung SJ, Shim CK, Kim DD (2010)

Characterization and in vitro evaluation of freeze-dried microparticles composed of

granisetron–cyclodextrin complex and carboxymethylcellulose for intranasal delivery. Int

J Pharm 400: 59-65.

19. Piao HM, Balakrishnan P,Cho HJ,Kim H, Kim YS, Chung SJ, Shim CK, Kim DD

(2010) Preparation and evaluation of fexofenadine microemulsion for intranasal

delivery. Int J Pharm 395: 309-316.

20. Lim HT, Balakrishnan P, Oh DH, Joe KH, Hwang DH, Lee YB, Yong CS, Choi HG

(2010) Development of novel sibutramine base-loaded solid dispersion with gelatin and

HPMC: Physicochemical characterization and pharmacokinetics in beagle dogs. Int J

Pharm 397:225-230.

21. Xuan JJ, Balakrishnan P, Oh DH, Yeo WH, Park SM,Yong CS, Choi HG (2010)

Rheological characterization and in vivo evaluation of thermo sensitive poloxamer-based

hydrogel for intramuscular injection of piroxicam. Int J Pharm 395: 317-323

22. Yoo JH, Shanmugam S, Thapa P, Lee ES, Balakrishnan P, Baskaran R, Yoon SK, Choi

HG, Yong CS, Yoo BK, Han K (2010) Novel Self-nanoemulsifying Drug Delivery

System for Enhanced Solubility and Dissolution of Lutein. Arch Pharm Res 33: 417-

426.

23. Li DX, Han MJ, Balakrishnan P, Yan YD, Oh DH, Joe JH, Seo Y, Kim JO, Park SM,

Yongand CS, Choi HG (2010) Enhanced oral bioavailability of flurbiprofen by the

combined use of micelle solution and inclusion compound. Arch Pharm Res 33: 95-101.

Prabagar Balakrishnan - Resume

24. Mu CF, Balakrishnan P, Cui FD,Yin YM, Lee YB, Choi HG, Chung SJ, Shim CK, Kim

DD (2010) The effects of mixed MPEG–PLA/Pluronic copolymer micelles on the

bioavailability and multidrug resistance of docetaxel. Biomaterials 31: 2371–2379

25. Balakrishnan P, Lee BJ, Oh DH, Kim JO, Lee YI, Kim DD, Jee JP, Lee YB, Woo JS,

Yong CS, Choi HG (2009) Enhanced oral bioavailability of Coenzyme Q10 by self

emulsifying drug delivery systems. Int J Pharm 374: 66–72.

26. Balakrishnan P, Shanmugam S, Oh DH, Yoo BK, Woo JS, Yong CS, Choi HG (2009)

Formulation and in-vitro assessment of minoxidil niosomes for enhanced skin delivery.

Int J Pharm 377: 1–8.

27. Balakrishnan P, Lee BJ, Oh DH, Kim JO, Hong MJ, Jee JP, Kim JA, Yoo BK, Woo JS,

Yong CS, Choi HG (2009) Enhanced oral bioavailability of dexibuprofen by a novel

solid SEDDS formulation. Eur J Pharm Biopharm 72: 539–545.

28. Balakrishnan P, Yoo BK, Woo JS, Kim JA, Rhee JD, Piao MG, Choi HG, Yong CS

(2007) Enhanced bioavailability of poorly water-soluble clotrimazole by inclusion with

β-cyclodextrin. Arch Pharm Res 30: 249-254.

29. Yong CS, Li DX, Balakrishnan P, Park BC, Yi SJ, Yoo BK, Lyoo WS, Woo JS, Rhee

JD, Kim JA, Choi HG (2007) The effect of β-cyclodextrincomplexation on the

bioavailability and hepatotoxicity of clotrimazole. Pharmazie 62: 756-759.

Domestic Papers

1. Cho HJ, Balakrishnan P, Lin H, Choi, MK, Kim DD (2013) Application of

biopharmaceutics classification system (BCS) in drug transport studies across human

respiratory epithelial cell monolayer. J Pharm Invest. 42(3).

2. Lee EA, Balakrishnan P, Song CK, Choi JH, Noh GY, Park CG, Choi AJ, Chung SJ, Shim

CK, Kim DD (2010) Microemulsion-based hydrogel formulation of itraconazole for topical

delivery. J Pharm Invest. 40: 305-311.

Prabagar Balakrishnan - Resume

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