Biochemistry

Farabi Nur

** ***** ******** 416-***-****

North York, ON M3A 3H4 *********@*****.***

EDUCATION

Bachelor of Science, Specialized Honours Biochemistry Expected completion: October

-2014

York University, Toronto, ON

Related coursework

• Molecular and Cellular Biology, Macromolecules, Biotechnology, Genetics, Organic and

Inorganic Chemistry, Biochemistry and Advanced Biochemistry, Cell and Molecular Biology of

Development, Proteomics, Biology of Cancer, Bioanalytical Chemistry

Laboratory

• Molecular Genetics: Enzyme activity of α-Amylase, Analysis of α-Amylase proteins – SDS-

PAGE and Western Blotting, Isolation of chromosomal DNA from Bacillus licheniformis, PCR

Amplification, Southern Hybridization and Detection, Restriction Enzyme cleavage, Cloning of

α-Amylase gene, Ligation of Chromosomal and Plasmid DNA, Transformation, Verification and

Mapping of α-Amylase clones.

• Worked in a team and independently - gained teamwork and time management skills. Lead group

discussions. Achieved critical, analytical, mathematical and problem solving skills – performed

laboratory calculations, prepared solutions and dilutions, interpreted statistical data and calculated

concentrations, kinetics and constants. Gained research skills from writing lab reports and

analyzing collected data. Maintained cleanliness, order and organization of laboratory.

Biochemistry research project

• Worked with yeast enzyme UBP15. Gained experience on Bacterial protein overexpression

systems and strategies, protein crystallization trials, GST gene fusion system, GST pull down,

PCR cloning, expression and purification of His-tagged proteins, yeast transformation, molecular

cloning from genomic DNA, plasmid construction, transformation of plasmids into electro

competent cells, isolation of plasmids from cells, transformation into yeast cells and test for

expression of proteins in yeast cells. Prepared results from experiments and made reports.

• Attained research and planning skills through creating ideas, identifying and solving problems,

analyzing issues, gathering information, developing strategies and meeting goals.

Presentation Topics

• “Protein Content Determination Techniques”

• “Molecular Beacon Aptamers for Direct and Universal Quantitation of Recombinant Proteins

from Cell Lysates”

• “UBP15 Potential Substrates and their interaction with its N-terminal domain”

EXPERIENCE

Sous Chef 2011

- 2012

Pimenton, Toronto, ON

• Duties included handling monetary transactions, maintaining store appearance, stocking

merchandise, maintaining inventory, customer service, managing calls, schedule meetings,

preparing ingredients for chef and maintaining a clean and organized store.

• Attained organizational skills through coordinating tasks, multitasking and handling details.

Gained communication skills through listening attentively, speaking effectively and openly

expressing ideas. Developed ability to work under high pressure by helping cater for weddings.

Sales Representative 2009 -

2011

Avon Canada Inc., Toronto, ON

• Duties included maintaining high levels of product and customer knowledge, arrange for delivery

of products, addressed customer’s needs, described products’ features and demonstrated usage of

products, received orders and invoicing.

• Ability to prioritize tasks and manage time through dealing with school work and part-time job.

Able to build and maintain relationships with customers, tactfully handling any complaints.

Nutrition Volunteer 2011 –

2012 Kensington Hospice, Toronto, ON

• Plated and served food to residents. Cooked and baked when needed. Maintained a clean and

organized kitchen.

• Acquired multitasking and organizational skills. Gained interpersonal skills through collaborating

with co-workers, being supportive to residents, understanding feelings, accepting responsibilities

and guiding new volunteers.

SKILLS

Laboratory skills and equipment:

• Biochemistry and Cell & Molecular Biology skills: pHmeter, micropipetting, DNA extraction,

DNA digestion and ligation, plasmid DNA preparation, DNA labelling, gene cloning, restriction

enzyme digests, PCR, QPCR, Southern blots, spectrophotometric analysis, prepared

polyacrylamide and agarose gels and ran through electrophoresis. Affinity chromatography, SDS-

PAGE, cell fractionation by differential centrifugation, protein expression and purification,

proteolysis assays, kinetic assays (Michaelis–Menten and single turnover), protein-DNA binding

assays, radioactive DNA-binding assays, Western blots. Bacterial culture plating methods.

• Biochemistry and Cell & Molecular Biology skills: strong working knowledge of PCR, QPCR,

SDS-PAGE, Southern blots and Western blots. Familiar with DNA extraction, digestion, ligation,

labelling and cloning. Proficient at proteolysis, preparing gels for electrophoresis, proteolysis

assays, kinetic assays

• Chemistry skills: solution preparation, titrations, extractions, filtrations, separations, simple and

fractional distillations, mp and bp determination, solubility tests, thin-layer chromatography,

liquid chromatography, reflux, recrystallization, resolution of racemic mixtures, using polarimeter

to measure optical activity, separation of organic mixtures on the basis of acidity and basicity.

• Histology skills: fixation, tissue processing, embedding, hemotoxylin and eosin staining,

sectioning, microtome, cryotome, coverslipping, decalcification, light microscopy, electron

microscopy, preparation of cells for cryopreservation.

• Physiology skills: spirometry, measuring hb concentration, ECG, blood pressure measurement,

auscultation, phonocardiography, sphygmomanometer and stethoscope, urinalysis

Computer skills:

• Proficient with the following softwares: RasMol, Pymol, Protein data bank, BLAST and

Chemdraw.

• Experienced using Microsoft Office Suite (MS word, MS Excel, MS PowerPoint).

Teaching Skills:

• Tutored students from middle school to high school in Mathematics and Science. Worked as a

teacher – O-level Mathematics and Science. Work included preparing notes, test papers and study

guides as well as earned administrative skills.

Language skills:

• Fluent in oral and written English and Bengali.

Extra-curricular activities

• Member of Biochemistry Association at York – 2009 to present.

• Member of Biology Society – 2009 to present.

• Member of Chemistry Society – 2009 to present.

• Volleyball and Badminton. Member of York Badminton Club – 2010 to 2011.

• Reading – science fiction, mysteries. Enjoy cooking, photography and travelling.

Contact this candidate