Data Assistant

Petro Ryabokon, MS

***** ****** **, ****** ******, CA 92677; phone: 716-***-****;

*********@*****.***

ACCOMPLISHED SCIENTIST offering experience in Multi-Color Flow Cytometry;

Assay Development and Validation; Managing of Multi-Parameter Experiments;

Statistical Data Analysis and Presentation. 11 years of bench experience in

biotechnology and academic research settings.

AREAS OF EXPERTISE

Flow Cytometry, Design and Execution of Cell and Tissue based assays,

Immunoassays, Cancer Biology, Stem Cells and Rare Cells Analysis,

Radioprotection, Biochemistry, Drug Discovery and Development,

Troubleshooting and Optimizing of Technical Protocols, Data Analysis and

Presentation, Oral and Written Communication, Diva, FCSexpress, Cell Quest,

FlowJo, Excel, GraphPad Prism, MS Office, Sigma Plot; Photoshop, SOPs and

Reports Writing.

LABORATORY SKILLS:

Flow Cytometry, Design of flow based cellular assays, cell immunostaining

protocols and experimental layouts (LSRII / Diva platform), Flow Cytometry

data analysis in FCS Express;

Cell separation from blood; blood and bone marrow stem cell

immunophenotyping by surface markers (up to 11 colors);

High throughput screenings, tissue culture, cell-based assays;

Immunological Assays, ELISA, LUMINEX, Immunofluorescence; Western Blotting,

2D electrophoresis, Solid Phase Binding Assays; Phage Display,

Immunoprecipitation, PCR, LUMINEX data analysis;

Liquid chromatography (immunoaffinity, size-exclusion, ion-exchange and

reverse-phase); Analytical centrifugation; Scanning Differential

Calorimetry; UV/Vis and fluorescence spectroscopy;

Purification of rRNA fragments and RNP-complexes. Protein expression,

extraction, purification and characterization.

EDUCATION

1996. M.S. in Molecular Biology. Institute of Protein Research,

Academic Center for Molecular Biology and Biotechnology,

Pushchino State University (Pushchino, Russia).

1989-1994 M.S. in Biochemistry. Kharkov State University (Kharkov,

Ukraine).

Professional Experience

2014: Flow Cytometry Specialist. Flow Data Analysis and consulting.

2012: Independent Contractor with DeNovo Software.

Flow cytometry software analysis

2006-2010: Cleveland Biolabs, Inc.; Cleveland, OH - Buffalo, NY

Scientist in Radioprotection department. Hematopoietic Stem Cell Project.

10/2007 - 08/2010

Key Highlights:

Found that the "600 series" of compounds are powerful inducers of

hematopoietic stem cells in bone marrow mobilizing them into peripheral

blood.

Data were presented on Gordon Research Conference: Stem Cells & Cancer,

2009, Switzerland.

Developed assays, protocols and SOPs for flow cytometry

experiments.

FACS measurements and data analysis

Supervised assistant scientists and technologists

Monitored literature

Evaluated pre-clinical data for Protectants CBLB502 and CBLB612 and wrote

reports

Studied mechanism of action of radioprotecting drugs

Scientist in Anti-Cancer department. 09/2006 - 10/2007

Key Highlights:

Found compounds capable to inhibit prostate cancer cells.

The data were published in peer-reviewed journal and presented on AACR

meeting.

Investigated molecular mechanism of Curaxins

Performed small molecule screenings in cell based assays. High throughput

screenings, ELISA, Western Blot, molecular cloning.

Research Consultant. 2006

Investigated molecular mechanism of apoptosis-modulating drugs.

2002-2003: Cleveland Clinic Foundation; Lerner Research Institute;

Cleveland, OH

Research Technologist in the Department of Molecular Cardiology

Investigated ligand binding properties of I-domain in ?M-, ?L- and ?D-

integrins using Solid Phase Binding Assays.

2001: Case Western Reserve University; Cleveland, OH

Research Assistant II Department of Nutrition

Studied IRES formation (Transcription in vitro, Gel-shift assay).

2000: Cleveland Clinic Foundation; Lerner Research Institute; Cleveland, OH

Research Technologist in the Department of Molecular Biology

Investigated transcription and splicing in vitro.

1997-1999: Institute of Protein Research, Russian Academy of Science;

Academic Center for Molecular Biology and Biotechnology, Pushchino State

University (Pushchino, Russia)

Post-Graduate Student; Senior Research technologist in Laboratory of

Nucleoprotein Physics

Studyied physical and chemical properties of the 5'-domain small ribosomal

subunit (Th.thermophilus, E.coli). The data were presented on International

Congress on Extremophiles

Publication and Presentation:

1) Natalya Narizhneva, Natalia Tararova, Petro Ryabokon, Inna Shyshynova,

Anatoly Prokvolit, Pavel Komarov, Andrei Purmal, Andrei Gudkov,

Katerina Gurova. Small molecule inhibitors of Androgen Receptor in

androgen independent prostate cancer: insight on transcription

independent function of Androgen Receptor. Cell Cycle (2009), v.

8(24): 4155-4167.

2) Alexander Shakhov, Eugenia Strom, Petro Ryabokon, Katerina Gurova,

Frederick Bone, Eugene Kononov, Vijay Singh, Elena Feinstein.

Protectan CBLB612 - a new agent for radical improvement of

hematopoietic stem cell proliferation and mobilization for treatment

of cancer and acute radiation syndrome. Gordon Research Conference:

Stem Cells & Cancer, September 13-18 2009, Les Diablerets,

Switzerland.

3) Natalya Narizhneva, Petro Ryabokon, Natalia Tararova, Leonid Brodsky,

Pavel Komarov, Vera Shakhova, Inna Shushunova, Andrei Purmal, Elena

Feinstein, Andrei Gudkov, Katerina Gurova. Inhibition of androgen

independent prostate cancer cells by small molecules. AACR annual

meeting, April 12-16, 2008, San Diego, CA, USA.

4) Peter Ryabokon and Igor Serdyuk. Physical and Chemical Studies of

the 5'-Domain of the Small Ribosomal Subunit Th. Thermophilus. The

International Congress on Extremophiles '98; Abstracts; p.198.

5) B.K.Gavrylyuk, T.I.Nikolaeva, Yu.A.Rochev, P.P.Ryabokon,

I.I.Selezneva.

The Collagen Gels, Chondroitin-4-Sulfate, Proteolytie Stability.

Biophysics (Moscow), N4, 1995, pp.1356-1358.

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