Senior Research Technologist

Anuradha Nadimpalli, Ph.D.

**** ****** *****

Crystal Lake, IL – 60014.

Contact # 815-***-****

Cell phone 815-***-****

E-mail **********@*******.***

With a background and experience in major fields of Biology, I am pursuing a job to use

my education, experience and good-team player skills to work on exciting and goal-

oriented projects.

Techniques:

• Experienced:

1. Taking initiative, standardizing and establishing new protocols, techniques and

assays to be used by lab personnel.

2. Metagenomic and Metatranscriptomic library preps

3. Bacterial gene knockout and knock-in using Recombineerning technique

4. Site-directed mutagenesis

5. Cloning and sub-cloning

6. PCR, qPCR and designing primers and cloning strategy

7. FISH (Florescent In-situ Hybridization)

8. QQQ LCMS/MS (Triple quadrupole liquid chromatography with tandem mass

spectroscopy) and GCMS/MS (Gas chromatography tandem Mass Spectroscopy)

9. LCM (Laser capture Microscopy)

10. Protein extraction and purification by Ion-Exchange chromatrography and FPLC

and running PAGE gels

11. Growth and maintenance of Obligate Anaerobes and BSL2 microbes

12. Microbial transformations and mammalian cell transfections

13. Genomic DNA, plasmid DNA and RNA extractions and purifications; Estimating

concentrations and purity by agarose gel electrophoresis and by Agilent

Bioanalyzer DNA and RNA chips.

14. Scale-up Fermentation

15. Tissue sectioning with cryotome

16. Maintaining and cataloguing bacterial, plasmid, primer, vector stocks for the lab.

Maintaining lab database of the same.

17. Procuring, storing and cataloguing patient tissue and stool samples for

experiments

18. Training and assisting lab colleagues and personnel from collaborating labs in

microbial culturing

19. General microbiology, molecular biology and biochemistry techniques

20. General lab maintenance, ordering supplies, maintaining lab records and

shipments

21. Participation in lab discussions and formulating new ideas and experiments with

an active contribution towards finishing the experiments.

22. Excellent computer skills – Microsoft Word, Excel and Powerpoint

• Familiarity:

1. Mammalian cell culturing

2. Western blots

3. Small animal (mice) work

4. ICPMS (Inductively coupled mass spectroscopy)

Professional Experience:

1. Senior Research Technologist (2010 – Present): Dr. Eugene Chang’s lab, GI

dept., University of Chicago, Chicago.

• Instrumental in developing, standardizing and preparation of Metagenomic and

Metatranscriptomic libraries to study gut microbiome structure and gene

expression.

• Developed and Established FISH (Florescent Insitu Hybridization) technique to

study spatial organization of gut microbiome.

• Standardized Recombineering technique to modify targeted microbial genomes.

• .Instrumental in purchase and establishment of Anaerobic Culturing chamber

for growing obligate anaerobes and BSL2 microbes. Developed Protocols to

grow anaerobes such as Bilophila wadsworthia, Fecaelibacterium prausnitzii,

Clostridium scindens, Bifido breve. Experienced in growing Lactobacillus GG,

Salmonella typhimurium, and various E. coli strains

• Took initiative and established SOPs to measure SCFAs (short chain fatty acids)

analysis by GC-MS-MS and bile acids by QQQ LCMS/MS

• Initiated, developed and established H2S analysis technique

spectrophotometrically

• Used techniques such as qPCR, LCM (laser capture microdissection), cloning

and subcloning to study gut microbiome

• Procure, catalogue and store human biopsy and stool samples for experiments as

part of HMP (Human Microbiome Project)

• Assist in DNA, RNA extractions and performing qualitative and quantitative

analysis using Agilent Bioanalyzer and DNA/RNA chips

• Assist in tissue sectioning using a cryotome, staining sections and performing

Laser Capture Microdissection

• Regularly assist lab personnel in mice experiments

• Actively participate in lab discussions and initiate new ideas and experiments

• Maintaining lab records, shipment details and ordering supplies

2. Research Technologist II (2005 – 2008): Dr. Robert Goldman’s lab, Cell and

Molecular Biology section, Feinberg School of Medicine, Northwestern University,

Chicago

• Cloned and sub-cloned various cytoskeletal proteins – lamins, actins, nestins etc

• Performed Site-directed mutagenesis of cytoskeletal proteins

• Protein purifications by IEC (ion exchange chromatography) and FPLC

• Running PAGE, agarose gels and Western blots

• Mammalian Cell culturing

• Transformations and Transfections

• LSM (Laser Scanning Microscopy) to visualize fixed and stained mammalian

cells

• Maintaining plasmids, vectors, competent cells and mammalian cell stocks for

general lab use

• Creating and maintain lab database for vectors, primers and plasmids using

VectorPro 2.0 programme

• Assisting in general lab maintenance, ordering supplies and keeping track of

shipments

3. Research Technologist I (2001- 2003): Dr. Thomas O’Halloran lab, Chemistry

dept., Northwestern University, Evanston Campus, Chicago.

• Performed site directed mutagenesis of SOD (Superoxide Dismutase) and CCS

(Copper Chaperon for SOD)

• Bacterial transformations and protein purification by IEC and FPLC

• Purifying plasmid DNA

• Running agarose, PAGE and western blots

• Assisting post-doctoral and graduate students in their work

• Routine lab work such as preparing competent cells, plasmids stocks, agar plates

and culture media

Education:

Ph.D. (Microbiology; 1995-2000): Osmania University, Hyderabad, India.

Worked towards Ph.D dissertation on Bio-leaching of metals from ore

concentrates by Thiobacillus ferrooxidans

Master’s (Biochemistry; 1993 – 1995): University of Hyderabad, India – Passed

in first division.

Bachelor’s (Genetics, Botany, Chemistry; 1990 – 1993): Osmania University,

India – Passed with distinction

Awards & Merits:

• Co-Author on “Devkota S, Wang Y, Musch MW, Leone V, Fehlner-Peach H,

Nadimpalli A, Antonopoulos DA, Jabri B, Chang EB. (2012). Dietary-fat-

induced taurocholic acid promotes pathobiont expansion and colitis in Il10-/-

mice. Nature.487(7405):104-8.

• Was over-all second in collage at the undergraduate level.

• Qualified GATE (Graduate Aptitude Test in Engineering) at the all-India level and

was awarded Junior Research Fellowship by the University Grants Commission

of India to carry out research work towards Ph. D degree.

• Awarded Senior Research Fellowship for overall performance and progress in

research.

• Presented poster at Association of Microbiologists of India held at Mangalore,

India, in 1999.

• Participated in 38th Annual conference of Association of Microbiologists of India

held at New Delhi, India, in 1998.

Referrals:

• Available upon request

Contact this candidate