Research Assistant

ARJUN REDDY BOLLAMPALLI

857-***-**** ● ** Peterborough St, Apt: 307, Boston, MA-02215

***********.*@*****.***.*** ● www.linkedin.com/in/arjunbollampalli/

August 18, 2014

Dear Hiring manager,

I am writing to apply for the ‘Research Technician’ position that is posted on the Dana Farber Cancer

Institute career website. I am a recent graduate with Master’s in Biomedical Sciences and a

specialization in Drug Delivery Systems from Northeastern University. I have excellent academic

background on Cancer Biology, cell biology and Drug delivery systems in addition I also have over

twenty months of experience as Research Associate at Dr. Vladimir Torchilin’s lab. I believe that my

experience, skills and passion will make me a strong fit for this position.

My Master’s program at Northeastern University gave me strong analytical abilities and lab skills which

got me selected as Graduate Research Associate at ‘The Center for Pharmaceutical Biotechnology and

Nanomedicine.’ I advanced my research and presentation skills while being a team member of this

strong research driven center. I gained experience in formulating and evaluating various Nano -drug

delivery systems. Working with ‘Antibody loaded formulations’ gave me ample opportunity to work on

Cell culture, Cell based assays, ELISA, Western blot, gel electrophoresis, chromatography and Animal

handling, which I believe are transferrable to current position. In addition, my critical thinking ability to

convert abstract data into PowerPoint and Word brought me compliments from peers.

Before joining Northeastern, I worked as a Summer Intern for Matrix Laboratories in India. It provided

me an opportunity to learn and work on Large scale manufacturing and Quality control units and also

record keeping.

As a young scientist I wish to make a positive difference in cancer therapy through my knowledge. I am

confident that I can bring my experience in cancer drug targeting and contribute to th e mission of Belfer

Institute in fight against Cancer. I would appreciate meeting with you to discuss my candidacy. Thank

you for your time and consideration. I will look forward for your response.

Yours sincerely,

Arjun Reddy Bollampalli

MS Pharmaceutical Sciences

Northeastern University

ARJUN REDDY BOLLAMPALLI

857-***-**** ● 75 Peterborough St, Apt: 307, Boston, MA-02215

***********.*@*****.***.*** ● www.linkedin.com/in/arjunbollampalli/

SKILLS:

Mammalian cell culture, Cell line maintenance, Cryopreservation

ELISA, Western blot and Gel-Electrophoresis

Cytotoxicity and Cell Viability Assays (MTT, Cell Titer Blue)

Cell based assay optimization, Medium-throughput screening of drug combinations

Plasmid preparation, Transfection (plasmid & siRNA)

Protein extraction, RNA extraction

Animal Handling: Mice oral, intravenous and intraperitoneal dosing

Mice anesthesia and euthanasia

Mice heart puncture, organ and tumor harvesting

Immunohistochemistry, TUNEL

FACS, Cryostat tissue sectioning

BCA and Fluorescence assays

Chromatography (Size exclusion, Affinity, TLC)

Immunomicelles, Lipid Nano particles preparation and characterization

Troubleshooting Windows and Mac operating systems

MS Office suite with adobe Photoshop and programming in C

EDUCATION:

Northeastern University Boston, MA

Master of Science in Biomedical Sciences (GPA: 3.57) May 2014

Courses: Concepts in Pharmaceutical Sciences, Pharmacokinetics and Drug Metabolism, Molecular Cell Biology,

Human Physiology and Pathology, Advanced Physical Pharmacy, Experimental Design and Biometrics.

Osmania University Hyderabad, India

Bachelor of Pharmacy (GPA: 3.65, First Division) May 2011

Relevant Courses: Pharmaceutical Technology, Pharmaceutical Formulations and Dosage Forms, Pharmaceutical

Engineering, Pharmacology and Pharmacotherapy, Medicinal Chemistry, Organic Chemistry.

Academic Recognitions:

Placed in top 5% of Graduate Pharmacy Aptitude Test-2010, national entrance test for Masters

EXPERIENCE:

Center for Pharmaceutical Biotechnology and Nanomedicine Northeastern University

January 2013 – Present

Graduate Research Assistant

Project: 1

Clarify endocytosis pathway for 2C5 antibody modified doxorubicin loaded immunomicelles in human

cancer cells using PC-3, HeLa, A549 and MCF7 cancer cell lines

Optimized doxorubicin uptake and IC50 studies in different cancer cell lines using BCA, Fluorescence and

FACS analysis

Performed regular ELISA, Western Blot, Gel electrophoresis to estimate the loading efficiency of antibody

onto micelles

Performed medium throughput screening of formulation with drug and inhibitor combinations using 96 well

cell based assay format

Maintained frozen stocks and performed passage of cells and cell seeding of 12,24,48,96 well plates

throughout the project time

Prepared, characterized and analyzed 2C5 and IgG antibody loaded micelles

Project: 2

Developing polymeric nano particles for siRNA delivery

Synthesized and analyzed siRNA loaded micelle-like nano particles and liposomes

Performed regular analysis of formulations on C166-GFP cell line using transfection and FACS

Conducted experiments independently on subprojects

Prepared cell inventory by freezing various cell lines

Team member of annual lab cell inventory inspection and maintenance.

Adept in using single and multi-channel micro pipettes, working with 12, 24, 48, 96 well plates and liquid

handling

Responsible for the maintenance and seeding of various cancer cell lines for experimental need of scientists in

the lab

Presented reports in meetings and group projects

Trained new students to work on the project

Matrix Laboratories Limited Hyderabad, India

June 2011 – November 2011

Summer Intern

Worked in Formulation and Quality Control departments

Supervised formulation, coating and polishing of tablets

Prepared logs and maintained batch to batch records

Formulated and evaluated Gastro-protective matrix tablets

Completed project “Formulation and Evaluation of Gastro Protective Matrix Tablet through Solid Dispersion

Technique”

ACTIVITIES:

Member of American Association of Pharmaceutical Scientists (NU Chapter) and Indian Pharmaceutical

Association

Member of Pharmaceutical Journal Club, Northeastern University

Volunteered for Infectious Diseases World Summit- 2014, Symposium on Drug Discovery and

Development in the 21st Century and ADVAMED-2012

Leader of Planning and organizing committee – “Xenium-11” -Successfully organized national level

pharmacy and cultural fest attended by over 5000 students and academicians

Contact this candidate