Engineering Marketing

Ram Narayanaswamy

* ******* **., ******** ***, NJ 08619 . Phone: 512-***-**** . Email:

***.******@*****.***

Key Accomplishments

* Team lead for the conception and development of a unique cell

microarray platform for high throughput imaging of cellular

phenotypes; Lead to filing of a patent

* Developed Orthogonal datasets to image based assays using shotgun

proteomics of supernatant and pellet based proteomes

* Streamlining technical and manufacturing components to develop

portable diagnostic kits for markets in Asia and Africa

* Team lead for the development of early cancer diagnostics

using artificial zinc finger proteins for prevention of tumor

malignancies at the Yale Medical School

* Won a pilot grant for the amount of $10,000 for next

generation sequencing of aptamer libraries for rapid development

of diagnostics and therapeutics

2010-2013 Albert Einstein College of Medicine

Bronx, NY

Research Fellow

Won a pilot grant for the amount of $10,000 for next generation

sequencing of aptamer libraries for rapid development of

diagnostics and therapeutics

* Purified and optimized protein production for protein

engineering efforts using IVC (In vitro compartmentalization);

Worked closely to set up Sortase enzymatic assays for IVC

experiments

* Performed SELEX for receptor mediated cell internalization;

conducted rigorous RNA focused experiments on oncologically

relevant receptor targets

* Implemented the selection of aptamer based reagents through a

single round of ligand enrichment using Next Generation

Sequencing

* Conducted validation assays using RNA interference/

Fluorescence activated cell sorting (FACS) and used UNIX shell

scripting and database analysis to analyze NGS datasets

resulting in reduced analysis time from 1 week through external

collaborators to a single day using in-house expertise

* Generated stable mammalian cell lines and used FACS and FloJO

to analyze aptamer binding to receptor targets

* Executed the first ever cell culture based targeted mRNA

delivery assay in aptamer derivatized liposomes

Business Courses

04/2011 - 06/2011

Intern - Technology Transfer Program, Office of Business &

Development: The Office of Business and Development acts as a

business mediator for marketing University Patent portfolios

with Bio-pharmaceutical companies; Responsibilities included

performing outreach with pharmaceutical companies, liaison

between university faculty and potential pharmaceutical clients,

Summarizing invention disclosures and preparing marketing flyers

2008-2010 Yale University School of Medicine

New Haven, CT

Postdoctoral Research Scientist, April 2008

Team lead for the development of early cancer diagnostics using

artificial zinc finger proteins for prevention of tumor

malignancies; Established collaborations and worked on writing

grants with groups inside Yale and at Albert Einstein College of

Medicine, NY, University of North Carolina, Chapel Hill, NC and

University of San Juan, PR

Research pursuits:

* Conducted zinc finger and DNA based immunoassays from

bacterial and mammalian cell expressed proteins; Cloned zinc

finger biosensors using Infusion technology

* Developed genetically encoded zinc finger based biosensors for

detection of cellular epigenetic signatures in tissue culture

* Developed luminescent-based assays and microscopic detection

of luminescent samples

* Co-authored a publication with biosensors reporting drug-

induced changes in epigenetic silencing in living cells by

binding to LINE-1 repetitive elements

* Future work to entail engineering of inteins that are DNA

dependent using IVC and/or rational protein design so as to

increase signal to noise ratio of the biosensor; Engineered

intein-based biosensors can facilitate development of amplicon

encoded protein nanofactories as well as a single locus

translational diagnostic.

.

Business Courses: Pursuing advanced financial and business

management courses; Participated in the Yale Biotechnology &

Pharmaceutical Society (YBPS) Consulting Case Competition;

Conversant with basic concepts of corporate finance and economic

principles including basic accounting and inventory management,

capital budgeting techniques, marketing strategies and

formulation of business plans.

Education University of Texas at Austin

Austin, TX

Ph.D. in Molecular Biology, 2008 (Advisors: Andrew Ellington and

Edward Marcotte)

Team lead for the conception and development of a unique cell

microarray platform for high throughput imaging of cellular

phenotypes. Applied cell microarrays towards identifying

previously unknown protein aggregation phenomenon in response to

nutrient starvation leading to novel drug targets for aging and

neurodegenerative diseases. Worked in tandem with multiple

laboratories at the Center for Systems and Synthetic Biology, UT

Austin

Courses/ skill sets:

* Cell based assays - High throughput imaging assays including

automated microscopy and image analysis resulting in

unprecedented collection of image based phenotypes (>10,000

images) in a single microscopy run

* Fluorescence in situ hybridization, Immunofluorescence, Signal

amplification techniques such as Hybridization chain reaction,

quantum dot probes and dendrimer probes

* Mass spectrometry based proteomics - Developed Orthogonal

datasets to image based assays using shotgun proteomics of

supernatant and pellet based proteomes

* Computational Biology - Bioinformatics and biological

databases

* Automation: Hands-on operation of Biomek FX and Tecan liquid

handling workstations

* Functional genomics using Transposon and group II intron based

knockouts in E.coli

* Examined the knockouts created by mariner transposons and

group II introns using TAIL-PCR

* Chemical Biology & Engineering - surface chemistry and

immobilization techniques,

laboratory automation and management of genome-wide libraries,

fermentation technologies and scale-up for manufacturing

Indian Institute of Science

Bangalore, India

Master of Science in Biological Sciences, 2001.

Molecular Cloning with Vector NTI; Recombinant protein

expression, mouse and rat dissections, antibody generation in

rabbits and IgG purification, Immunohistochemistry and

immunofluorescence

University of Delhi Delhi,

India

Bachelor of Science in Biochemistry, 1998

Internships Foundation for Innovative and Novel Diagnostics (FIND)

(2008)

Technical Consultant: Expert analysis on promising early

stage diagnostic technologies for transition to

commercialization; Focus on streamlining technical and

manufacturing components to develop portable diagnostic kits

for markets in Asia and Africa

International Center for Genetic Engineering and Biotechnology

(Summer 1997)

Summer Intern: Worked as a team to improve and refine

protocols for recombinant Hepatitis B vaccine development;

optimized fermentation protocols such as pH control, Oxygen

tension for the mass production of recombinant antigen in

yeast; Vaccine was adopted for clinical trials and marketing

Skills

Molecular Cloning Nucleic acid extraction/ analysis

Library construction Automation/ Liquid handling

Animal handling/ mammalian cell Yeast cloning

culture

Microbiological techniques Protein Purification

PCR/ RT-PCR/ RACE Protein Assays

Protein Expression Stable Cell lines

Transient Transfection ELISA/ Immunoassays

Automated epifluorescence & Western Blotting/ LiCOR

Confocal Microscopy

Luminescence and Fluoresence Mass Spectrometry

based bioassays/ FACS

Protein separations/ HPLC Unix Shell Scripting

Immunofluorescence/ FISH Statistics (SPSS)

Databases (SQL) JAVA, PERL, R programming

Statistical Tools (Excel, Sigma

Plot)

Associations/

Certifications

* HUPO (Human Proteome Organization) certified workshop in mass

spectrometry-

based proteomics, MALDI, LC/MS, sample preparation, data

generation and

data analysis

* American Association for Cancer Research

* American Society for Gene and Cell Therapy

* Grant Writing Certification from the Yale University School of

Medicine

Publications,

Awards

/Honors Invited Reviewer for PLOS One, 2012

Huang X, Narayanaswamy R, Fenn K, Szpakowski S, Sasaki C, Costa

J, Blancafort P, Lizardi PM. DNA Cell Biol. 2012 Feb 7 Sequence

specific biosensors report drug-induced changes in epigenetic

silencing in living cells

Rammohan Narayanaswamy, Levy M, Tsechansky M, Ellington A.D,

Marcotte E.M

Proceedings of the National Academy of Sciences June 4, 2009

Widespread reorganization of metabolic enzymes into reversible

assemblies upon nutrient starvation

Rammohan Narayanaswamy, Moradi E.K., Niu W., Hart G.T., McGary

K.L., Ellington A.D., Marcotte E.M. Journal of Proteome Research

2008 Dec3 (EPub ahead of print)

Systematic Definition of Protein Constituents along the Major

Polarization Axis Reveals an Adaptive Reuse of the Polarization

Machinery in Pheromone-Treated Budding Yeast

Rammohan Narayanaswamy, Niu W., Scouras A., Hart G.T., Davies

J., Iyer V.R., Andrew D. Ellington, Edward M. Marcotte

Genome Biology 2007; 7(1): R6. Epub 2006 Jan 31s

Systematic profiling of cellular phenotypes with spotted cell

microarrays reveals mating pheromone response genes

Insuk Lee, Rammohan Narayanaswamy, Edward M. Marcotte.

Methods in Microbiology 2007; (36): 597-628

Bioinformatic prediction of yeast gene function

Cell Microarray for profiling of cellular phenotypes and gene

function(UTAU 1073; Patent filed).

Studying Proteome wide dynamics in yeast in response to

pheromone by using cell microarrays and machine learning

Rammohan Narayanaswamy Keystone Symposia for Systems Biology and

Proteomics, Steamboat Springs, CO - March 2007), Keystone

Symposia Travel Fellow

Rammohan Narayanaswamy and Andrew D. Ellington

Handb Exp Pharmacol. 2006; (173):423-45. Review

Engineering RNA based circuits

Cell microarrays as a tool for dissecting cellular function"

Rammohan Narayanaswamy YGM 2006, Princeton University,

Princeton, NJ - July 2006

Yeast cell microarrays - emerging new technologies" Rammohan

Narayanaswamy Yeast Genetics & Molecular Biology (YGM 2006)

Conference, Princeton university, Princeton, NJ - July 2006

Invited podium speaker

David & Mary Miller Fellow, UT Austin (2006)

Systematic Profiling of Cellular Phenotypes in yeast using a

Cell Microarray reveals new pheromone response genes" Rammohan

Narayanaswamy Keystone Symposia for Systems Biology and

Proteomics, Denver, CO - April 2005

IISc Research Scholar (1998)

Activities Volunteer for Asha (Yale), Climbing Club (UT Austin), Nature

Club (IISc, Bangalore)

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