BRIAN CURRAN Res: 703-***-**** / Cell: 703-***-****

**** ****** ****** *****, ******, VA 20194 acetb3@r.postjobfree.com

VIRGINIA TECH GRADUATE

BACHELORS OF SCIENCE IN MICROBIOLOGY AND IMMUNOLOGY

College of Science-Major Department- Biology

MCLA Division 1 Lacrosse Player

Highly accomplished student athlete, looking for a job to further my career opportunities in line with my chosen

academic field of training and fields relating to and expanding upon it. I plan on going to graduate school next

year and am looking for research experience that my current research and laboratory skills as well as specific

knowledge can be utilized in a productive manner as well as help to develop my skills within my area of study that

will be vital to my future professional career.

EDUCATION

BACHELORS OF SCIENCE IN BIOLOGY WITH AN OPTION IN MICROBIOLOGY AND IMMUNOLOGY VIRGINIA TECH, Blacksburg, VA

HIGH SCHOOL DIPLOMA IN COLLEGE PREP, HERNDON HIGH SCHOOL, HERNDON VA

CORE QUALIFICATIONS

Leadership & Team Building Academic Over Achiever Dedicated Student

Accomplished Athlete Responsible Hard Worker Amiable Personality

Focused and Dedicated Responsible and Team-Oriented Ambitious

SKILLS AND EXPERIENCE

- EXPERIENCED WITH BASIC LABORATORY AND AESPETIC TECHNIQUES AS WELL AS ADVANCED LABORATORY TECHNIQUES AND

PROCEDURES INCLUDING BUT NOT LIMITED TO: ENZYME ASSAYS, PHAGE TITRATION, SPECTROPHOTOMETRIC ASSAYS, VARIED USE OF

AGAR PLATES/MEDIUMS AND ANTIBIOTIC RESISTANT MARKERS, PROTEIN OVERPRODUCTION, METAL AFFINITY CHROMATOGRAPHY, LIQUID

CHROMATOGRAPHY, MASS SPECTROPHOTOMETRY, SDS-POLYACRYLAMIDE GEL ELECTROPHORESIS, GENETIC COMPLEMENTATION OF

MUTATIONS, DNA PURIFICATION, TRANSPOSON MUTAGENESIS, MUTANT SCREENING, BLOTTING TECHNIQUES, CHROMATOGRAPHIC

TECHNIQUES, GENE EXPRESSION ANALYSIS, GRAM-STAINING, ELISA, AND ARBITRARY PRIMED PCR WITH SEQUENCE ANALYSIS.

- SENIOR FINAL RESEARCH PROJECT INVOLVED USING TRIPARENTAL MATING TO INTRODUCE THE TN-5 -110 TRANSPOSON INTO

SINORHIZOBIUM MELILOTI THROUGH THE MOBILIZABLE PLASMID PJG110, SCREENING FOR IMMOTILE MUTANTS USING SWIM PLATE

ASSAYS, PURIFYING THE CHROMOSOMAL DNA, THEN USING ARBITRARY PRIMED PCR TO AMPLIFY THE TRANSPOSON INSERTION SITES

ALONG WITH AGAROSE GEL ELECTROPHORESIS IN ORDER TO IDENTIFY THE EXACT SITE OF THE TN-5 INSERTION SITES WITHIN THE

MOTILITY GENE OF S. MELIILOTI USING DNA SEQUENCING REACTIONS AND COMPARING OUR SEQUENCES TO THAT OF A GENOME

SEQUENCING DATABASE.

- GENERAL UNDERSTANDING OF GENETIC CONCEPTS AT BOTH MICROBIAL AND HUMAN LEVEL.

- ADVANCED KNOWLEDGE OF METABOLIC PATHWAYS AND CELL PHYSIOLOGY INCLUDING TRANSPORT MECHANISMS AND CHEMICAL CELL

RESPONSES.

- UNDERSTANDING OF MECHANISMS OF PATHOGENICITY OF MANY COMMON PATHOGENIC BACTERIA AS WELL AS THE USE OF ANTIBIOTICS

AND THEIR ROLE IN MODERN MEDICINE.

- DETAILED KNOWLEDGE IN THE HUMAN BODY’S IMMUNE RESPONSE AND DEFENSE MECHANISMS.

- PRE-MEDICAL MAJOR WITH GENERAL KNOWLEDGE AND EXPERIENCE LEARNED THROUGHOUT SUCH AN UNDERGRADUATE DEGREE.

- SKILLED WITH BOTH WINDOWS AND MAC SOFTWARE, ESPECIALLY MICROSOFT OFFICE APPLICATIONS SUCH AS WORD, EXCEL, AND

POWERPOINT.

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WORK EXPERIENCE

Cripple Creek Country Club, Millville, DE 05/2012-7/2012

Worked with Club Groundskeeper on golf course maintenance.

Clyde’s of Reston 6/2009 – 12/2011

Worked as busboy, server, and waiter during summer breaks and holidays.

United States State Department 7/2009

Shadowed staff member in the State Department.

Selected Achievements and Accomplishments

Graduated with Advanced Biology Option in Microbiology/ Immunology from College of Science

4 year College MCLA Division 1 Lacrosse player on 2-time MCLA Southeast Lacrosse Conference

Champions

2014 Southeastern Lacrosse Conference All Tournament Team on 2014 SELC Champion

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