Project School

Kuili Fang

********@***.*** • 951-***-**** • *** W Big Springs Rd, CA 92507, USA

EDUCATION

M.S.,Chemical and environmental engineering, University of California, Riverside Expected in Jun. 2014

B.S.,Biomolecular engineering, East China University of Science and Technology Graduate in Jul. 2012

WORKING SKILLS

Microorganism culture and fermentation, gene clone, gene electrophoresis gel, SDS-PAGE, western blot, ELISA, yea st surface

display

Protein expression, purification and refolding, antibody production and selection, immunology reaction, fluorescence protein

production

Operations of microscope, spectrophotometer, flow cytometer, PCR, centrifugatiion

Computer skills: VB, C, C++, C#, Matlab, Origin, Office2010, pymol

RESEARCH EXPERIENCE

Project leader, Therapeutic protein lab of CEE department of the UCR May. 2013 -present

Project: Using yeast surface display to select the antibodies which functionally inhibit the Matrix Metalloproteinase14 ( MMP14)

Clone different anti-MMP14 antibody gene libraries to plasmid pCTCON-2 and transform to the yeast cells

Produce fluorescence MMP14 and N-TIMP2,

Express anti-MMP14 antibodies at the yeast surface which then detected by ELISA, microscope and FACscan

Antibodies and antigen MMP-14 immunology reactions, and fluorescence was scanned by FACS

Project leader, Therapeutic protein lab of CEE department of the UCR Feb. 2013 - Apr.2013

Project: Antigen and inhibitor protein expression and inhibition test of N-terminal domain of tissue inhibitor of

metalloproteinases 2 (N-TIMP2)

Clone N-TIMP2 gene into plasmid pET32a, and express, purify and refolding N -TIMP-2

Test antigen Matrix Metalloproteinase14 ( MMP14) and N-TIMP2 inhibition reaction by FRET assay, get IC50 32 nM and

observe the fluorescence by spectrophotometer

Produce inhibition product N-TIMP2-MMP14 complex and analyze the crystal structure about residue interactions by pymol

Project assistant, Erythromycin fermentation lab of the ECUST Aug. 2011 - Mar.2012

Project: Kinetic constants and model equations of growth and production for the Saccharopolyspora HE1086E3 during different

growth phases by Matlab

Culture the cells in 5L fermenter for 48 h, and test the productions by HPLC

Simulate the final equation to get the constants by Matlab, and compare the simulation curve wit h the theory curve.

Optimize the conditions for the fermentation and achieve better production

WORK EXPERIENCE

Activity leader Student association of Bioengineering School of ECUST Jul. 2008 - Aug. 2010

Lead the school science and technique show

Lead the visit of the nation labs of the plants

HONORS AND AWARDS

2008 Most Creative Award of the school science and technique show

2009 The Second Prize of ping pong school competition

2010Standard speaking Chinese 2A

2010Interesting study of blackbody radiation second prize

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