Assistant Manager

Yue Huang

**** ********** ****, *** **, Redondo Beach, CA 90278

206-***-****, *************@*****.***

OBJECTIVE

Seeking an entry level scientist position to apply my knowledge and skills

on analytical chemistry, especially on HPLC/UPLC and mass spectrometry.

Looking for a relocation opportunity to California to join my family.

EDUCATION

Ph.D.: October, 2013

Department of Chemistry, University of Washington, Seattle, WA GPA 3.75/4.0

B. Eng.: July, 2008

Pharmaceutical Engineering, Beijing Institute of Technology, Beijing, China

GPA 3.6/4.0

TECHNICAL EXPERTISE

. Extensive experience in multiple techniques used in proteomics including

nano-LC trapping column and analytical column packing with home build

system, two dimensional LC separation with MS/MS identification, peptide

database search, tandem MS labeling, i-TRAQ. Protein sample preparation

including purification, digestion and desalting and gel-electrophoresis

of proteins, peptides and DNA.

. Extensive experience in operation of mass spectrometers including Thermo

LCQ, LTQ, Velos, Orbitrap, and LTQ-FT; Waters Synapt MS, Quadrupole and

microflow LC, AB Sciex Q-Star MS and Bruker MALDI-TOF. Extensive

experience with mass spectrometer maintenance including

inserting/removing/cleaning ion funnel/octopoles/pump and other daily

maintenance work.

. Extensive experience in micro fabrication in clean house: spin coating,

exposing, metallization, plasma enhances chemical vapor deposition, dry

etching and wafer dicing;

. Experience in organic synthesis

. Extensive experience in developing ionization source for mass

spectrometer including MALDI, ESI, NSI and SAWN

. Software skills: TPP (Trans-Proteomic Pipeline), Mascot, Sequest, Comet,

Labview, AutoCAD, Microsoft Office and Adobe Acrobat. Extensive

experience with instrument software: Xcalibur, Masslynx, Empower and

Chemstation.

. Language skills: English, Chinese (Mandarin) and Japanese.

HIGHLIGHTS OF EXPERIENCE

. Solid background in pharmaceutical engineering. Skillful in proteomics

and lipidomics.

. Extensive experience in MS operation and MS instrumental development

including ionization methods, instrumental design and engineering. Skills

in micro fabrication, chip designing. Assisted in proteomics projects for

medicinal chemistry mass spectrometry center.

. In depth knowledge in analytical chemistry, mass spectrometry, separation

science, micro fabrication, medicinal chemistry, pharmacokinetics and

pharmacy.

. Independent problem solving experience including research design of

experiments.

AWARDS

. Graduate Student Travel Award, International, Department of Chemistry

(2012)

. Excellent Undergraduate Thesis, Beijing Institute of Technology (2008)

. First Class Scholarship, Beijing Institute of Technology (2004-2008)

PROFESSIONAL EXPERIENCE

2014.03 - now Scientist I (Manager: Jianmei Kochling)

Quality Sciences and Analytical Technology (QSAT), Genzyme through

ProUnlimited, Framingham

. Development of analytical support for of therapeutic protein drugs,

including forced degradation studies, stability test and

characterization.

. Characterization of therapeutic protein drugs by a LC/MS based

analysis platforms

2014.01 - 2014.03 Post-Doctoral Researcher (Advisor: Dr. David R. Goodlett

and Dr. Youngah Goo)

Department of Pharmaceutical Sciences, University of Maryland, Baltimore

. Training of new group member in Micro-Fabrication and Instrumentation.

. Proteomic analysis in School of Pharmacy proteomics center.

2010.09 - 2013.10 Research Assistant (Advisor: Dr. Frantisek Ture?ek and

Dr. David R. Goodlett)

Department of Chemistry and Department of Medicinal Chemistry, University

of Washington, Seattle

. Characterization of surface acoustic wave nebulization (SAWN) as

ionization method for MS and analyzing lipid, protein and peptides

. Developing SAWN-Digital Microfluidics for sample analysis

. Proteomics Studies for the mass spectrometry center

2008.12 - 2010.09 Research Assistant (Advisor: Dr. Norman J. Dovichi)

Department of Chemistry, University of Washington, Seattle

. Analyzing bacteria extracted protein sample with new proteomics

methods with tandem mass tag iTRAQ

2008.07 - 2013.12 Teaching Assistant

Department of Chemistry, University of Washington, Seattle

. Excellent teaching evaluation for general and organic chemistry and

senior classes in instrumental analysis.

2007.07 - 2007.09 Summer Intern

Hubei Huazhong Pharma Co, Wuhan, China

PUBLICATIONS

1. "Surface Acoustic Wave Nebulization Produces Ions with Lower Internal

Energy than Electrospray Ionization", Y. Huang, S. H. Yoon, S. R. Heron,

C. D. Masselon, J. S. Edgar, F. Ture?ek and D. R. Goodlett Journal Of The

American Society For Mass Spectrometry, 2012, 23, 6, 1062-1070

2. "Surface Acoustic Wave Nebulization Facilitating Lipid Mass

Spectrometric Analysis" S. H. Yoon, Y. Huang, J. S. Edgar, Y. S. Ting, S.

R. Heron, Y. Kao, Y. Li, C. D. Masselon, R. K. Ernst and D. R. Goodlett

Analytical Chemistry, 2012, 84 (15), 6530-6537

3. "Surface Acoustic Wave Nebulization", in press

4. "Standing Wave SAWN Increasing Ionization Efficiency", in preparation

5. "SAWN-DMF for phosphor protein analysis", in preparation

PRESENTATIONS

1. Seminar Talk "Introduction to SAWN-MS", University of Edinburgh, 2012.7.

2. "Defining Limits of Detection for Surface Acoustic Wave Nebulization

(SAWN) MS", Y. Huang, J. D. Chapman, S. H. Yoon, S. R Heron, C. Masselon,

J. S. Edgar, N. Isoherranen, F. Ture?ek, D. R. Goodlett, ASMS 2012,

Vancouver, Canada

3. "Surface Acoustic Wave Nebulization (SAWN) MS: Detection Limit of a New

Soft Ionization Method", Y. Huang, J. D. Chapman, S. H. Yoon, S. R Heron,

C. Masselon, J. S. Edgar, N. Isoherranen, F. Ture?ek, D. R. Goodlett,

MSBM 2012, Dubrovnik, Croatia

4. "From Caffeine To Protein: The Detection Of A Wide Range Of Molecules By

Surface Acoustic Wave Nebulization (SAWN) Mass Spectrometry" Y. Huang,

S.H. Yoon, J.S. Edgar, S. Heron, C. Masselon, F. Ture?ek and D.R.

Goodlett, MicroTAS 2011, Seattle, WA

5. "Comparison of internal energy deposition with Surface Acoustic Wave

Nebulization (SAWN) and Electrospray Ionization" Y. Huang, S.H. Yoon,

J.S. Edgar, S. Heron, C. Masselon, F. Ture?ek and D.R. Goodlett, ASMS

2011, Denver, CO

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