Plant Assistant

Pratibha Ghimire

**** **** ***** *****, *** ***, Plymouth, MN 55441

Phone: 662-***-****, E-mail: ********.*******@*****.***; *****@*******.***

LinkedIn: www.linkedin.com/in/pratibhaghimire/

CAREER OBJECTIVE: To find an entry level position in the fields of biochemistry and

biological sciences and apply gained knowledge and laboratory skills

LABORATORY SKILLS:

• Molecular Biology:

Isolation of DNA, RNA and proteins from plants, animals and microorganisms

RT-PCR, PCR, gel purification of DNA fragments, bacterial transformation, plasmid

purification, restriction digestion, DNA sequencing, sequence analysis(ORF-Finder,

BLAST, CDD, CLUSTAL, MUSCLE, MEGA)

Agarose gel electrophoresis, SDS-PAGE, silver staining

Northern Blotting, Western Blotting, ELISA

Biochemistry:

Protein extraction and purification

Column and Paper Chromatography, TLC, Dialysis, Spectrophotometer, Gel filtration

Serological techniques (Agglutination, Immunodiffusion, ELISA)

Handling different centrifuge instruments, pipetting techniques, pH meter

• Microbiology:

Isolation of microorganisms, biochemical characterization, optimization of nutritional

level, temperature, and pH of different microorganisms, plate reader

• Plant Pathology/Virology:

Identification and characterization different plant viruses using molecular techniques,

purification of virus from plant tissue, dsRNA extraction, mechanical transmission of

virus

• Analytical:

Qualitative and quantitative analysis of different substance

Extraction and analysis of high density and low density oil from different plants (leaf,

seed)

• Tissue Culture:

Meristem culture for producing virus free plants

COMPUTER SKILLS:

MS Office suites: Word, Excel, PowerPoint and Outlook

WORK EXPERIENCE

Pratibha Ghimire

9620 37th place north, Apt 108, Plymouth, MN 55441

Phone: 662-***-****, E-mail: ********.*******@*****.***; *****@*******.***

LinkedIn: www.linkedin.com/in/pratibhaghimire/

Mississippi State University, Starkville, MS

Graduate Research Assistant [Aug 2011-Dec

2013]

Research in molecular characterization of novel virus associated with Blackberry Yellow Vein

Disease (using molecular biological techniques)

Determine the incidence of different viruses associated with Blackberry Yellow Vein Disease in

wild and cultivated blackberries in Mississippi

Pratibha Ghimire

Phone: 662-***-****

SANN International College, Kathmandu, Nepal [Jan 2010-July

2011]

Laboratory Instructor for Undergraduate Biotechnology Program

• Supervise practical classes and exams for undergraduate students for biochemistry,

microbiology and molecular biology

• Help students to gain essential laboratory skills for biotechnology studies

• Assist the professors with biochemistry course work and grading

RLABB (Research Laboratory for Agriculture, Biochemistry and Biotechnology),

Kathmandu Nepal

Research Assistant [Jan 2009-

Dec 2009]

• Biochemical characterization of different microorganisms

• Isolation of microorganisms via plating (spreading and streaking) broth and slant

• Optimization of nutrition level, temperature, and pH of different microorganisms

EDUCATION:

Masters of Science in Agricultural life Sciences (concentration Biochemistry) [Jan

2014]

Mississippi State University, Starkville, MS

Thesis: Study of viruses associated with the Blackberry Yellow Vein Disease in Mississippi (Plant

Virology)

GPA: 3.53/4

Bachelor of Science in General Biochemistry [Dec

2009]

Universal Science College, Pokhara University, Kathmandu, Nepal

GPA: 3.33/4

Project Work: screening of sodium azide induced mutants of actinomycetes.

Membership

• Student member: American Phytopathological Society (APS)

• Nepal Biotechnology Association (NBA)

Participation

• Oral presentation at APS Annual meeting 2013, Baton Rouge, Louisiana “Partial

Characterization of novel virus in Blackberry Yellow Vein Disease”

• Oral presentation at MAPPAN Annual meeting 2012, 2013 (Mississippi Association of

Plant Pathologists and Nematologists)

• Presentations in seminars conducted in department of Biochemistry, Molecular Biology,

Entomology and Plant Pathology, Mississippi State University

Pratibha Ghimire

Phone: 662-***-****

Employment Authorization

• Permanent Resident

•

Pratibha Ghimire

Phone: 662-***-****

References:

Sead Sabanadzovic

Professor, Mississippi State University

Mississippi State University, Starkville, MS

Email: *************@**********.*******.***

Phone: 662-***-****

Nina Aboughanem

Research Assistant Professor, Mississippi State University

Mississippi State University, Starkville, MS

Email: *****@*******.***

Phone: 662-***-****

Priya Prasai

PhD Candidate

Louisiana State University Health Sciences Center

Email: ******@******.***

Phone: 318-***-****

Contact this candidate