Manager Engineering

Address: **-** ******* ***., ***.*K Elmhurst, NY *1373

Cell Phone: 646-***-****,

Email: **********@*****.***, ******@********.***

Work Experience

. 2011~present, Work as postdoctoral researcher in Columbia

University Medical Center (CUMC), Orthopedic Surgery Department

. 2010~2011, Work as facility manager for Jeol 2100 TEM instrument in

Hunter College, CUNY and already gave training and lectures for ten TEM

users.

Education

. 2010, Ph.D., Chemistry, Chemistry Department, Hunter College, City

University of New York (CUNY), NYC, NY, USA.

. 2004, transferred Graduate student, Chemical Engineering, Department of

Chemical Engineering, Colorado School of Mines, Golden, CO, USA.

. 2003, M.S., Analytical Chemistry, Changchun Institute of Applied

Chemistry (CIAC), Chinese Academy of Sciences (CAS), Changchun, Jilin

Province, China.

. 2000, Bachelor, Chemical Engineering, Department of Chemical

Engineering, Tsinghua University, Beijing, China.

Honors and Awards

. 2009~2010, A free two-year sponsored membership of AAAS/Science for

excellence in science.

. 2008~2009, Sponsored Dissertation Fellowship, Graduate Center (GC) of

CUNY, as Distinguished Graduate Student.

Expertise and Skills

. Instrument:

Microscopy:

TEM (transmission electron microscopy), including EDX and EELs,

experience for over eight years and over five types from the four

manufactures,

SEM (scanning electron microscopy), experience for five years and

two types,

AFM (atomic force microscopy) and its supplemental functions

(electronic field microscopy- EFM and magnetic field microscopy- MFM),

experience for seven years.

Spectral:

Circular Dichroism (CD), Raman, FTIR, Fluorescence, UV-vis-NIR

(previous five had experience for over eight years), DLS (dynamic light

scattering) and XRD.

Analytical:

HPLC, SDS-Page, Westernblot, PCR and Electrochemistry.

. Material Science:

Fabrication of Crystal Nanowires and Nanoparticles in broad

material range: Metal/Semiconductor (Au, Ag, ZnO, TiO2, SiO2 and

ZrO2).

. Biochemical:

Cell Culturing. Protein Expression, Purification and Analysis. DNA

Transformation and Analysis.

. Organic:

Synthesis of C7 peptide monomer and C7 self-assemble-tubes.

. Computer:

C/C++, Visual C++, Visual Basic, Fortran, Matlab, Origin and CAD.

Research Experience

. 2011~present Postdoctoral Researcher, CUMC, New York, USA.

Advisor: Prof. Francis Y. Lee

. Bone Biology: inhibition of osteoclast by calreticulin protein or

parathyroid hormone to cure osteoporosis

o Investigate endoplasmic reticulum calreticulin(CRT) to block the

signal pathway generated from receptor activator of NF-?B (RANK)

in order to inhibit the differentiation of osteoclast precursor

cells to matured osteoclast, experiment in vitro and in vivo.

o Study the mechanism and effect of intermittent uptake of

parathyroid hormone (PTH) to prevent bone mass loss and inhibit

osteoclast in vivo.

o Investigate the PTH to affect Erk pathway in cells to reduce the

inflammation in bone remodeling in vivo and in vitro.

. Exploration of Nanomaterials in Bone Repair and Remodeling

o Making periosteum-like membrane by artificial collagen-like triple

helix protein and phosphate nanomaterials to speed up spongy or

woven bone formation after damage and promote the transformation

to cortical bone in remodeling stage.

o Scaffold engineering through the materials of hydroxyl apatite

(HA), PCL and chitosan or other polysaccharides, nanoparticles

were used to increase the strength.

. Clinical Treatment for Segmental Bone Defect at Critical Size and

Osteochondral Defect (Dept. of Defense Project)

o Biogenic autograft scaffold designed by 3D Mimics Software

according to the data of CT and MRI scannings.

o In vitro physical and mechanic study of scaffold in critical size

was used on hound's imperfect tibia and femur to mimic

osteodefection.

o In vivo study of same kind of scaffold in live hound, the effect

of bone growing factor included in biogenic scaffold on the bone

repairing and remodeling.

o In vitro study of scaffold in critical size in cadaveric human

femur and tibia.

o Make ready-to-use biogenic scaffold optimized from previous

research in clinical treatment.

. 2005~2010, Ph.D. Research, Hunter College, CUNY, New York, USA.

Advisor: Prof. Hiroshi Matsui

. Material Science: fabrication and synthesis through biomineralization

strategy

o Investigate the artificial collagen-like proteins (F877 and 242 in

various size) as biomimic template to make rigid and uniform-size

metal or semiconductor nanowires.

o Figure out specific peptides from protein library which have the

strongest ability to capture and mineralize precursor ions.

o Pre-bind specific peptides on the sidewall of collagen-like

protein templates and make precursors accumulate and crystallize

to form nanowires through biomineralization.

o Synthesis C7 bolaamphiphilic peptide monomer and make them self-

assemble to nanotubes under certain pHs.

o Utilize C7 monomer as nano-reactors to synthesize

metal/semiconductor crystal nanoparticles.

o Coat precursors onto C7 self-assemble-tubes to make

metal/semiconductor tubes by pre-binding similar specific peptides

on sidewalls of nanotubes.

o Use HRTEM and EDX to study the material- coated nanowires or

nanotubes.

. Biochemistry:

o Design the sequence and corresponding gene codes of a new

artificial collagen-like triple helix protein 242 by recombinant

technology.

o Synthesize 242 gene by three consequent parts and connecting them

by endonuclease enzyme to form final gene sequence.

o Incorporate the gene into DNA vector, transform vector to E.Coli.

cells, clone and purify cells.

o Harvest either F877 or 242 protein from E.Coli. culturing and

expression, and do protein purification and analysis.

o Revise the entire purification protocol in cooperation lab to

improve protein yield and purity.

. POM (polyoxometalate) materials as Store Device:

o Use conductive AFM tips to apply potential to the monolayer POM

film spread on Au substrate and use EFM to study the surface

character of reduced/oxidized regions.

o Use HRTEM, SEM and EDX to study the crystallized POM.

. 2001~2003, Master Research, CIAC,CAS, Changchun, China. Advisor:

Prof. Shaojun Dong (Member of Chinese Academy of Science Board)

. Electrochemistry and Spectroscopic:

o Study the interaction between proteins and small biomolecules by

in-situ spectro-electrochemical method which incorporate

electrochemical with circular dichroism/UV spectral techniques.

o Investigate the interaction of protein and DNA in self-assemble

membrane by in-situ spectro-electrochemical method.

. Mathematic Modeling:

o Develop SVDLS (single value decomposition and least square)

mathematics model and the computer program to estimate the second

structure of protein through deconvoluting the circular dichroism

spectral data.

. 1998~2000, Bachelor Work, Tsinghua, Beijing, China. Advisor: Prof.

Yong Jin (Member of Chinese Academy of Science Board)

. Fluid Mechanics:

o Use hydrogen gas and Petroleum Gas as tracing gas to study the

fluidized-reactor, sample from different heights of reactor and

analyze component by GC.

o Model and study the fluid behavior by programming Fortran program.

. Thermodynamic:

6 Build thermodynamic model of reactant particles in fluidized-reactor and

predict product and yield.

Teaching Experience

. 2004~present, Teaching Assistant, at Hunter College and LaGuardia

Community College, CUNY, New York, USA. Courses included elemental

chemistry, organic chemistry, physical chemistry and analytical

chemistry. Excellent oral and written communication abilities in English.

Academic Reviewer Experience

. 2009~present, Peer Reviewer for academic manuscript. Over ten academic

journals included Langmuir, Chem.Comm., J Mater. Chem., Chemistry of

Materials, Colloid and Surface:B, etc.

Publications

. 2013:

. N. Nuraje, H. Bai, K. Su, "Bolaamphiphilic Molecules: Assembly and

Applications", Review Article, Progress in Polymer Science, 38, 302-

343 (2013), equal contribution with first author.

. Y. Pan, Z. Wei, H. Bai, L. Pan, M. Sohel, M. C. Tamargo, J. R.

Lombardi, "PbCl2-oleylamine system for air-stable PbSe quantum dots

synthesis", J. Mater.Chem.C, submitted.

. P. J. Kaur, R. Strawn, H. Bai, K. Xu, Y. Xu, H. Matsui, "The self-

assembly of a collagen like fibrils with axial repeats of a designed

triple helix with periodic sequences for the charged and the

hydrophobic residues", submitted.

. F. Bian, H. Bai, B. Burton-Pye, L. Francesconi, "A New type of

information storage device: POM and their coordination polymers", in

preparation.

. 2012:

. Y. Pan, H. Bai, L. Pan, Y. Li, M. Tamargo, M. Sohel, J. R. Lombardi,

"Size Controlled Synthesis of Monodispersed PbTe quantum dots: Using

oleylamine as Capping Ligand", J. Mater.Chem., 22, 23593 (2012).

. 2011:

. P. Kaur, H. Bai, H. Matsui, "Genetically Modified Collagen-like

Triple Helix Peptide as Biomimetic Template". as a chapter of book

"Hybrid Nanomaterials: Synthesis, Characterization, and Applications",

Publisher: John Wiley & Sons, New York, 2011,Chap.9, p251-268, equal

contribution with first author.

. 2009:

. H. Bai, F. Xu, L. Anjia, H. Matsui, "Fabrication of ZnO Nanowires at

Far Below Room Temperature by using Genetically Modified Collagen-like

Triple Helix Peptide Nanowires as Biomimetic Template", Soft Matter,

5, 966 (2009).

. F. Xu, V. Volkov, Y. Zhu, H. Bai, A. Rea, N. Valappil, W. Su, X. Gao,

I. Kuskovsky, H. Matsui, "Long Electron-Hole Separation of ZnO-CdS

Core-Shell Quantum Dots", J. Phys. Chem. C, 113, 19419 (2009).

. 2008:

. L. Yang, N. Nuraje, H. Bai, H. Matsui, "Crossbar Assembly of Antibody-

Functionalized Peptide Nanotubes via Biomimetic Molecular

Recognition", J. Pep. Sci., 14, 203 (2008).

. 2007:

. H. Bai, K. Xu, Y. Xu, H. Matsui, "Fabrication of Au Nanowire in

Uniform Length and Diameter using a New Monodisperse and Rigid

Biomolecular Template, Collagen-like Triple Helix", Angew. Chem. Intl.

Ed., 46, 3319 (2007) (which was selected as a hot paper in April

2007).

. 2005:

. X. Jiang, Z. Zhang, H. Bai, E. Wang, S. Dong, "Effect of Electrode

Surface Microstructure on Electron Transfer induced Conformation

Changes in Cytochrome C Monitored by in situ UV and CD

Spectroelectrochemistry". Spectrochimica Acta A, 61A(5), 943 (2005).

. 2004:

. H. Bai, X. Liu, Z. Zhang, S. Dong, "In-situ Circular Dichroic

Electrochemical Study of Bilirubin and Bovine Serum Albumin Complex",

Spectrochem Acta A, 60, 155 (2004).

. 2003:

. H. Bai, X. Jiang, S. Dong, "Analyzing the in-situ

Spectroelectrochemical Circular Dichroism (CD) Spectra of Protein by

the method of Singular Value Decomposition and Least Squares (SVDLS)",

the Computer and Applied Chemistry, 20, 731 (2003).

. 2002:

. J. Zheng, Z. Li, A. Wu, H. Zhou, H. Bai, Y. Song, "The structural

transition of DNA-Tris (1, 10-phenanthroline) cobalt (III) complexes

in ethanol-water solution", Biochem. Biophys. Res. Comm., 299,910

(2002).

Conference Presentations

. H. Bai, C. Chandhanayingyong, C. Lee, F. Lee, Poster Presentation,

"The Usage of PCL-HA Scaffold on the Osteoconduction of Canine Tibial

Segmental Defect at Critical Size", ORS Annual Meeting, San Antonio,

TX, January 26-29, 2013, 1551.

. Y. Pan, Z. Wei, H. Bai, B. Cai, H. Zhang, M. C. Tamargo, J. Lombardi,

Poster Presentation, "Selenium from micrometer to nanometer, 244th ACS

National Meeting & Exposition, Philadelphia, PA, August 19-23, 2012

(2012), PHYS-428.

. D. Oh, F. Lee, A. Hsu, H. Bai, S. Nizami, H. Goto, H. Tawfeek, J. Kim,

S. Choi, Poster Presentation, "Physiochemical Dual Action Bone-like

Scaffold - Pilot Study in Canine Model", ORS Annual Meeting, San

Francisco, CA, Feb.4-7, 2012, 0628.

. Y. Pan, H. Bai, J. R Lombardi, Poster Presentation, "New, simple

method to synthesize lead telluride (PbTe) nanocrystals", 241st ACS

National Meeting & Exposition, Anaheim, CA, March 27-31, 2011 (2011),

COLL-303.

. H. Bai, P. Kaur, Y. Xu, H. Matsui, Poster Presentation,

"Biomineralization strategy for fabrication of nanometer-size

devices", NY ACS YCC Research Symposium, New York, NY, March 19, 2011

(2011).

. P. J. Kaur, M. Aruggoda, H. Bai, S. Wong, Y. Xu, Poster

Presentation,"Biophysical characterization of the self-association of

collagen triple helix", NYAS Conference, Protein Structure, St. Lewis,

MO, July 2009.

. H. Bai, H. Matsui, Oral Presentation, "Genetically-Modified Collagen

Triple Helix Proteins for Room Temperature Semi- conductor Nanowire

Synthesis", 237th ACS National Meeting & Exposition, Salt Lake City,

UT, March 2009 (2009),COLL-321.

. H. Bai, H. Matsui, Oral Presentation, "Self-assembled Peptide

Nanotubes for Electronics and Sensor Devices", 237th ACS National

Meeting & Exposition, Salt Lake City, UT, March 2009, PMSE-393.

. H. Bai, Y. Xu, H. Matsui, Poster Presentation, "Fabrication of

Semiconductor Nanowires at Far Below Room Temperature by Using

Genetically Modified Collagen-like Triple Helix Peptide Nanowires as

Biomimetic Template", Gordon Conference, Tilton, NH, July 2008.

. H. Bai, H. Matsui, Oral Presentation, "Fabrication of metal and

semiconductor nanowires in monodisperse size by using a genetically

modified collagen-like triple helix peptide as biomimetic template",

236th ACS National Meeting & Exposition, Philadelphia, PA, August 17-

21, 2008 (2008), POLY-566.

. H. Bai, H. Matsui, "Fabrication of metal and semiconductor nanowires

in monodisperse size by using a genetically modified collagen-like

triple helix peptide as biomimetic template", Polymer Preprints

(American Chemical Society, Division of Polymer Chemistry) (2008),

49(2), 868.

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