Customer Service Quality Control

BRIAN D. COLEY

**** ******** ******, *** ** • Greensboro, NC 27407 • 336-***-**** • acegoe@r.postjobfree.com

EDUCATION

University of North Carolina Greensboro Greensboro, NC

M.S. in Biochemistry, August 2014 (expected); B.A. in Biology, August 2006.

RESEARCH EXPERIENCE

University of North Carolina Greensboro, Department of Chemistry and Biochemistry

Graduate Researcher, Advisor: Dr. Norman Chiu November 2012 - present

Development and optimization of a fluorometric solid-phase assay for the detection of

Argonaute (AGO) 2 protein.

Quantification of microRNA incorporated into the AGO2 ribonucleoprotein complex from in

vitro samples using real time PCR analysis.

University of North Carolina Greensboro, Department of Biology

Undergraduate Researcher, Advisor: Dr. Karen Katula August 2005 - May 2006

Cell culture of E. Coli cells.

Transfected E. Coli cells with X-gal plasmid vector containing Wnt-5a gene sequence.

Harvested E. Coli cells containing transfected plasmid vector and used to prepared new cell

lines.

DNA extraction from E. Coli cells.

Agarose gel electrophoresis of cellular DNA extracts.

Purification of plasmid DNA from agarose gel using resin-based methods.

.Determination of Dickkopf-1 protein expression in 3T3-L1 cells.

TEACHING EXPERIENCE

University of North Carolina Greensboro, Department of Chemistry and Biochemistry

Graduate Teaching Assistant, General Chemistry Laboratory January 2013 – present

PROFESSIONAL EXPERIENCE

LabCorp of America Burlington, NC

Laboratory Technologists Team Leader July 2009 - January 2013

Molecular Genetics Department

Enforced quality control by upholding GCLP, CLIA, and CAP standards and ensured

adherence to SOP protocols by all molecular technologists.

Coordinated the daily work for technologists in the lab.

Ensured that scheduled time frames were met for testing benches, and relayed any pending

delays to departmental customer service.

Collaborated with upper management to re-evaluate current workflow trends and determine

areas at which efficiency and throughput could be increased.

Addressed scheduling needs and re-assigned technologists accordingly to circumvent any

possible lapse in patient testing.

Reviewed paperwork and results entered by technologists prior to reporting finalized patient

results to clients.

LabCorp of America Burlington, NC

Technologist September 2006 - January 2013

Molecular Genetics Department

Validation of a protocol for testing low-volume patient HPV samples using the Qiagen

Hybrid Capture Assay.

Validation of the Protodyne Radius HPV automated prep system protocol.

DNA extraction from patient samples.

Detection of Human Papilloma Virus (HPV) from extracted patient samples using the

Qiagen Hybrid Capture HPV assay and Hologic ASR Assay.

Detection of Methicillin-Resistant Staphylococcus Aureus (MRSA) virus from extracted

patient samples using real-time PCR as the end point detection.

Genotyping of HPV strains 16 and 18, using the Hologic Invader Assay.

SKILLS AND TECHNIQUES

ELISA Mass spectrometry PCR

Electrophoresis HPLC Cell

culture

Nucleic acid extraction UV/Vis spectrometry Molecular

Cloning

AWARDS AND HONORS

Laboratorian of The Year Award April 2010

LabCorp of America, Burlington, NC

Bruce M. Eberhart Award May 2006

University of North Carolina Greensboro Department of Biology

CONFERENCE PRESENTATIONS

B. D. Coley, Norman H. L. Chiu, A Fluorometric Microtiter-based Assay For The Detection of

Argonaute 2 Protein. Poster presented at the Central North Carolina American Chemical Society

Poster-Vender Night, Greensboro, NC, April 2014.

M. S. Nye, B. D. Coley, et. al., Comparison of HPV Detection Among Women Age 30 and Older

with NILM Cytology using Screening and Genotyping Tests from Two Different Manufacturers.

Poster presented at the Association for Molecular Pathology Conference, San Jose, CA, November

2010.

B. D. Coley, K. S. Katula, Expression of the Dickkopf-1 Protein in Differentiating 3T3-L1 Cells.

Oral presentation delivered at the North Carolina Academy of Science 103 rd Annual Meeting,

Bennett College, Greensboro, NC, March 2006.

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