Staton L. Wade, Ph.D.

National Institute of Environmental Health Sciences

*** *.*. ********* **.

Research Triangle Park, NC 27709

Phone: 804-***-****

acegaj@r.postjobfree.com

SUMMARY OF QUALIFICATIONS

• Research scientist with more than 11 years of laboratory experience and expertise in miRNAs, genomics,

molecular genetics, and epigenetics.

• Recipient of the NIH Fellows’ Award for Research Excellence in 2013 and an invited talk at the

International Keystone Symposium on Eukaryotic Transcription in 2012.

• Extensive experience with miRNA- and RNA-based assay development and optimization and genomic

technologies including next generation sequencing library construction, experimental design and analysis.

• Ability to communicate research needs and results effectively to colleagues and clients, coordinate multiple

ongoing research projects, analyze and interpret complex biological data, and write scientific reports and

protocols.

EDUCATION

2009 Ph.D. Biochemistry and Molecular Genetics, University of Virginia

2006 M.S. Biological Sciences, University of Virginia

2002 B.S. Biology, Distinguished Majors Program - High Distinction, University of Virginia

RESEARCH EXPERIENCE

2010-present Postdoctoral Research Fellow, Laboratory of Molecular Carcinogenesis, National Institute

of Environmental Health Sciences, National Institutes of Health

Mentor: Trevor K. Archer

• Independently developed and conducted research to study the regulation of chromatin

remodeling complexes by microRNAs in human embryonic stem (ES) cells.

• Designed studies, analyzed data, and interpreted experimental results to address

complex biological questions leading to the identification of novel regulatory

mechanisms controlling self-renewal and differentiation in hES cells.

• Developed and optimized biochemical assays to analyze RNA and DNA including

primer design, qRT-PCR, miRNA expression using qRT-PCR and Nanostring

technology, chromatin immunoprecipitation and next generation sequencing for ChIP

and RNA expression analysis.

• Prepared libraries and aided in data analysis and interpretation for ChIP-seq and RNA-

seq experiments.

• Performed DNA and RNA QC and quantification.

• Reported scientific results at lab and departmental meetings. Facilitated discussions

and provided ideas and constructive feedback to investigators working on diverse

research projects.

• Completed the NIEHS Introduction to Biostatistics and Bioinformatics Short Courses.

2004-2010 Graduate Research Associate, Biochemistry and Molecular Genetics, University of Virginia

Mentor: David T. Auble

• Developed a research project from a single piece of genetic data to the establishment of

novel connections between metabolic sensing and DNA repair pathways that led to

publication in an international journal.

• Developed cell-based assays for qRT-PCR, flow cytometry and DNA repair analysis.

• Initiated the use of new techniques including procurement, set-up, and maintenance of

laboratory equipment, and development and validation of new analytical procedures.

RESEARCH EXPERIENCE (continued)

• Communicated results and project goals to principal investigator and colleagues on a

regular basis. Participated in team meetings to provide feedback on technical issues

and project strategy.

• Completed a graduate-level course in Bioinformatics Analysis.

2003-2004 Acting Manager, Laboratory Specialist, Molecular Biology Core Facility, Massey Cancer

Center, Virginia Commonwealth University Medical Center

• Took initiative as the acting manager of a core facility offering DNA extraction,

molecular cloning, plasmid construction, and library preparation.

• Established relationships and communicated directly with customers to identify

technical requirements, define deliverables and report results.

• Designed and implemented complex molecular cloning projects and performed large-

scale plasmid DNA extraction and QC analysis. Completed projects within established

timelines.

• Implemented a new budgeting, billing, and data management system that streamlined

the input of customer project requests and invoicing.

• Developed and authored standard protocols that increased DNA yield by 50%.

• Managed procurement of laboratory instruments including evaluating needs,

researching options and communicating with vendors and technical specialists.

• Maintained laboratory equipment and managed laboratory inventory within a budget.

2001-2002 Undergraduate Researcher, Biochemistry and Molecular Genetics, University of Virginia

Mentor: David T. Auble

• Pioneered the use of new high-throughput genetic screening technologies that led to

the identification of novel genetic interactions in budding yeast.

• Set up a complete screening system, created novel strains, and developed a refined

screening protocol that resolved the technical issues of available screening libraries.

• Maintained accurate records of research studies and reported research findings.

Communicated research plan and results in distinguished majors’ seminar series and

undergraduate thesis.

TECHNICAL SKILLS

Cell Biology: human primary stem cell culture, human primary keratinocyte cell culture, in vitro cellular

differentiation, embryoid body formation, general cell culture (microbial and mammalian), cryopreservation,

flow cytometry, fluorescence activated cell sorting, fluorescence microscopy

Molecular Biology: RNA and DNA extraction and isolation, RNA and DNA quantification and QC analysis

PCR, qPCR, electrophoresis, molecular cloning, northern blotting, southern blotting, PCR-based DNA repair

assays, primer design

Biochemistry: protein extraction, cellular fractionation, SDS-PAGE, western blotting, enzymatic assays,

immunoprecipitation, chromatin immunoprecipitation, immuno-based DNA repair assays

Genetics and genomics: RNAi, next generation sequencing, ChIP-seq, RNA-seq, microarray analysis, genomic

data analysis and interpretation, high-throughput genetic screening, microRNAs, epigenetics

Technical Software: FlowJo, FACSDiva, Ingenuity Pathway Analysis Software, Partek Genomics Suite

PUBLICATIONS

Wade S.L. and D.T. Auble. (2010) The Rad23 ubiquitin receptor, the proteasome and functional specificity in

transcriptional control. Transcription. 1: 22-26.

Wade S.L., K. Poorey, S. Bekiranov, and D.T. Auble. (2009) The Snf1 kinase and proteasome-associated

Rad23 regulate UV-responsive gene expression. EMBO Journal. 28: 2919-2931.

Staton L. Wade, Ph.D. Page 2

ADDITIONAL SKILLS & EXPERIENCE

Leadership and Team Skills:

• Managed a team to plan and organize all aspects of one of the largest Career Fairs in the Triangle area.

• Coordinating efforts across multiple subcommittees, institutional administration and contractors to

ensure timely completion of goals, smooth execution of strategic planning and adherence to budget.

• Trained junior laboratory personnel, including 3 undergraduates, 1 technician and 4 graduate students.

• Assisted in the hiring process including interviewing and evaluating potential new lab personnel.

• Led trainees in planning award competitions, fund raising events, weekly seminar series, career

development workshops, scientific research symposia and numerous social networking events.

• Served as liaison between graduate students and University faculty.

• Completed management training including Management Boot Camp, Workplace Dynamics workshop

and Conflict Resolution workshop presented by the NIH Office of Intramural Training and Education.

Communication Skills:

• Served as a Guest Writer for the NIEHS Environmental Factor newsletter.

• Edited scientific manuscripts as a contract editor for American Journal Experts.

• Presented findings at local, regional and international scientific conferences, including 12 poster

presentations, 8 formal scientific talks and an invited talk at an international symposium.

• Authored 15 abstracts, 5 grant proposals and 5 scientific articles.

• Prepared and presented lectures for the Molecular Basis of Cancer section of a graduate genetics course

(BIOG 5120, Department of Biology) at North Carolina Central University.

• Completed a five-day Research Proposal Writing workshop presented by The Grantsmanship Center.

AWARDS AND HONORS

2013 Fellows’ Award for Research Excellence (FARE), National Institutes of Health

2012 Invited Talk, Keystone International Symposium on Eukaryotic Transcription

2008 Poster Award, Huskey Research Exhibition, University of Virginia

2002 Graduation with High Distinction, Department of Biology, University of Virginia

2000 Research Experience for Undergraduates Internship Program, National Science Foundation

2000 Intermediate Honors, University of Virginia

PROFESSIONAL SERVICE

2012-present NIEHS Trainees Assembly, Steering Committee, NIEHS

2012-2013 Co-Chair, 16th Annual NIEHS Biomedical Career Fair Planning Committee, NIEHS

Spring 2012 Guest Lecturer, Graduate Genetics, North Carolina Central University

2011-present Ad hoc reviewer for Journal of Biological Chemistry and Experimental Cell Research

2011-present NIEHS Biomedical Career Fair Planning Committee, NIEHS

2011 Summer Internship Program Seminar Series Planning Committee, NIEHS

2007-2008 Virginia Piedmont Regional Science Fair Judge, Charlottesville, VA

2007-2008 President, Graduate Biosciences Society, University of Virginia

2006-2007 Treasurer, Graduate Biosciences Society, University of Virginia

Fall 2005 Teaching Assistant, Medical Biochemistry, University of Virginia School of Medicine

PROFESSIONAL SOCIETIES

• International Society for Stem Cell Research

• NIEHS Trainees’ Assembly

• American Association for the Advancement of Science

• University of Virginia Graduate Biosciences Society

Staton L. Wade, Ph.D. Page 3

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