Customer Service Quality Control
Resume:
Anne B. Thomas
Hanover, MD 21076
Mobile:301-***-****
Email: ***********.****@*****.***
EDUCATION:
Master of Science in Biotechnology.
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**/****, ***** ******* University (JHU)
Bachelor of Science in Material Science and Engineering.
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05/2004, University of Maryland at College Park
SKILLS:
Skill Name Skill Level Last Used/Experience
Nucleic Acid Extraction Assays Excellent Currently used
Lab Equipment Precision &
Excellent Currently used
Calibration
3730 DNA Sequencing Excellent Used
SAP EXO,PCR, Gel Used
Excellent
Electrophoresis
PBMC Separation & Currently used
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Excellent
Cryopreservation
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DNA Prep for 3730 Used
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Excellent
Library
ELISA Assay Excellent Currently used
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SUMMARY OF QUALIFICATIONS:
• 7 years of experience as Biotechnologist.
• Skilled lab personnel experienced in 3730 DNA sequencing, PCR, Nucleic Acid Extraction,
ELISA Assay, PBMC Separation and Cryopreservation.
• Accurate, reliable, diligent and focused on the timely, quality completion of all lab
procedures. Work well under pressure and time constraints within high volume
environments.
• Observes and follows Good laboratory Practices (GLPs), Aseptic techniques and safety
precautions.
• Remarkable ability to prepare SOPS and record accurate laboratory results.
• Excellent ability to work independently, evaluate situations and act appropriately.
• Excellent interpersonal skills to interact courteously and effectively with co workers, lab
personnel and other outside contacts.
• Ability to pay careful attention to detail and to adhere to written protocols.
Automated Lab Devices Used:
Instrument Operation
Horiba ABX micros 60 Cell Analyzer
Luminex 200 Analyte Detection in biological samples
QIAcube RNA Extraction
ND 1000/2000 Measure DNA/RNA concentration
Spectrophotometer
Beckman Coulter Biomek Liquid handler for DNA
FX cell lysis
Pixsys(liquid dispenser) Liquid Dispenser for big
dye
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Thermo CRS Robot for cell lysis and
DNA Extraction
Applied Biosystems 3730 3730 DNA Sequencing
Analyzer
Micro fills Liquid Dispenser
GeneAMP 9700 PCR Thermal Cyclers
system for 384 well Plates.
ChemiDOC XRS + System Gel Imaging System
Hudson Plate Cranes Liquid Dispenser and
Plate Organizer
Titertek Liquid Dispenser
PROFESSIONAL EXPERIENCE
Lab Associate (2013 to 2014)
MedStar Health Research Institute Baltimore, Maryland
Contract: National Institute on Aging(NIA)
Receives/ process samples for various age related studies like BLSA, HANDLS,
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EXENDIN, VALIDATE etc.
Process blood, serum,urine and fecal samples based upon various clinical research
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protocols.
Processing includes plasma, buffy coat separation, and other sample aliquots to be stored
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in NIA bio repository inventory database like HARBOR PROD.
Performs sample inventory/re inventory on sample aliquots.
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Separate PBMC’s(Peripheral Blood Mononuclear cells) from whole blood and apheresis
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packs.
Separated cells are subjected to cryopreservation using freezing media and cryo rate
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freezer.
Cell counting of separated PBMC’s using Horiba ABX Micros 60 to calculate recovery.
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Runs different levels of blood controls and performs precision, calibration on Horiba ABX
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Micros 60.
Uses X Map Technology on Luminex 200 system to measure protein expression levels.
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Prepare 96 well plate to detect human cytokines such as IL 6, IFN g and TNF
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α, Leptin/MCP 1, Adiponectin and ICAM/VCAM.
Ran plates for collaborators for detection of Mouse cytokines, performs Q.C and monthly
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maintenance.
Runs different levels of blood controls and performs precision, calibration on Horiba ABX
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Micros 60.
Conducts sample processing by strictly adhering to aseptic techniques check list.
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Attends customer service calls, sample send outs and responsible for general
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maintenance of lab.
Lab Associate (2010 to 2013)
MedStar Health Research Institute Baltimore, Maryland
Contract: National Institute on Aging(NIA)
Handle DNA/RNA samples from longitudinal studies like BLSA(Baltimore
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Longitudinal Study on Aging).
Also, process DNA/RNA samples of NIA’s other aging related research studies like
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EXENDIN,HANDLS etc.
Perform manual and automated DNA/RNA isolation from human blood, saliva.
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Measure, track, maintain DNA/RNA concentration and inventory.
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Experiments are conducted in accordance with approved methods and procedures
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related to DNA/RNA.
Experimental Assays are conformed to established protocols from Clinical research
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branch(NIA IRP/CRB).
Scientific results are analyzed to determine its accuracy and validity.
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Identify unusual or unexpected results to determine its cause.
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Discuss findings, summaries, unusual results and conclusions with senior staff.
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Manage DNA/RNA sample processing requests upon priority from P.I’s.
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Experimental methods and techniques are modified or redesigned to adapt to
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unexpected developments in a research project.
Performs laboratory procedures like preparing alcoholic solutions, reagents,
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buffers and media.
Oversee Nucleic acid extraction assays in DNA/RNA lab.
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Utilize statistical analysis methods like regression, correlation in lab Quality control
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calculations to analyze Q.C data.
Maintain accurate lab records in hard and soft copy formats to ensure proper
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retention of data.
Organize and maintain research laboratory facilities and equipment by daily
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monitoring of room temperatures, equipment maintenance logs.
Calibrates and repairs ND 1000/2000 spectrophotometers and other lab
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equipments as part of PM(preventative maintenance).
Recommended, new equipment like high capacity centrifuge, for use in the
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laboratory.
Train laboratory staff and post docs in experimental assays and lab equipments.
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Properly handle biological samples under BSC’s(Biological Safety Hood) to avoid
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contamination.
Use Biorepository database like HARBORPROD to input DNA/RNA data.
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Use Automated Analyzers like QIAcube to extract DNA/RNA from whole blood,
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supernatants and tissue samples.
Organize, track and order lab supplies like buffers, reagents and media.
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Biotechnologist II (2006 to 2008)
Craig Venter Institute, Rockville, Maryland
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Promoted to this position.
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Performed DNA sequencing on Applied Biosystems 3730 analyzer.
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Process and React template DNA on 384 well plates using big dye for 3730 DNA
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sequencing.
Trouble shoots procedural and technical problems on 3730 sequencing analyzers
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as well as other automated devices in the lab.
Performed routine maintenance on the sequencers.
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Prepared bulk amount of precipitating reagents and checked for its Q.C.
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Performed 3730 re sequencing by SAP EXO, big dye terminator reactions.
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Involved in DNA isolation for 3730 DNA library by making bacterial cultures, pick
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bacterial colonies and isolate DNA.
Biotechnologist I (2005 to 2006)
J. Craig Venter Institute, Rockville, Maryland
Isolate template DNA for further processing by sequencing lab.
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Sets up automation devices for DNA precipitation process.
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Track and organize DNA freezer copies according to the required libraries.
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Maintained lab equipment and troubleshoot/resolved instrument problems.
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Responsible for preparing standard operating procedure(SOPS).
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Mastered Tracker II software for uploading processed templates.
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Lab Assistant (2004 to 2004)
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University of Maryland, College Park, Maryland
Prepared liquid crystal samples
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Examined liquid crystal samples on microscope to learn its structure.
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Computer Lab Assistant (2000 to 2004)
University of Maryland, College Park, Maryland
Assisted International Students and other lab users in use of Microsoft Applications.
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Perform clerical and support services as needed, such as answering the telephone.
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Maintained Computer lab and perform general lab duties.
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Certificates/Continuing Education:
PD BiopharmaInst GLP GLPOOS1: Professional Designation in Good Lab Practices.
Biopharma Institute Online, May 12 2014.
GLPQ02 IQ OQ: Quality Control: IQ/OQ and Calibration of Lab Equipment.
Biopharma Institute Online, December 31 2013.
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