Project Medical
Resume:
Manthankumar V Patel
Scranton, PA
**************@*****.***
Objective: A strong inclination to join a scientific research firm/facility which requires innovative and
analytical skills where I can utilize my professional and academic skills for potential growth of
respective institution.
EDUCATION:
Drexel University College of Medicine Philadelphia, PA
16th May, 2014
Master of Science in Molecular Medicine
Relevant courses: Fundamentals of Molecular Medicine I+II, Immunology, Immunopathology and
Pathobiology, Virus Pathogenesis, Bacterial Pathogenesis and Antibiotics, Parasitic Infection and Neglected
tropical diseases, Mycology and Mycoses, Cancer Biology, Biostatistics in Veterinary Science, Biotechniques
and Laboratory Research, use of Animal Models in Biomedical Science.
Marywood University Scranton, PA
9th May, 2010
Bachelor of Science in Biology
Relevant courses: Anatomy and Physiology I+II, Microbiology and Lab project, Capstone project.
M.B. Patel Science College Anand, India
Associate of Science in Biological Science June, 2006
Relevant course: Biology I+II, Zoology and Lab, Botany and Lab, Biochemistry I+II and Lab, Biotechnology
I+II and Lab, Microbiology and Lab.
Laboratorical Skills:
Molecular Biology
DNA/RNA isolation, Recombinant DNA technology, Plant and Bacterial plasmid isolation, PCR, Western Blot
analysis, Protein concentration assays, Spectrophotometry, nuclear and plasma membrane protein extraction,
Silver stain, Glycosylation stain, SDS-PAGE, DNA-Agarose Gel electrophoresis, Chromatography, ELISA,
Immunoprecipitation
Microbiology
Mammalian cell Culture (MDA231, T47D, HEK293T, 3T3L-1 cell lines), membranous fractioning, Caspase
cleavage and phosphorylation study for small proteins, Bacterial transformation, Cell transfection, Operating
under sterile condition, Media preparation, Antibiotic Susceptibility tests, and Various microbiological tests
include Microbial Identification by elimination tests, making suspensions, culturing bacterial cells in preferred
selective media such as EMB (Eosin-methylene blue) agar plate, Mac Conkey: for gram negative bacteria and
MSA (Mannitol Salt Agar) plate for gram positive bacteria.
Experience:
The commonwealth Medical College (Dr.Jun Ling) Scranton, PA
Research Student, Department of Basic Sciences Aug2012-Present
1. Research Project “Identification of eIFs (eukaryotic translational initiation factors) as new
circulating biomarkers for the diagnosis of the prostate cancer.”
Urine and Blood samples collection from prostate cancer patients and preparation for screening of
putative marker from numerous available proteins
Gradient (4-12% SDS) and traditional SDS-PAGE.
Wet and semi dry transfer
Western blot analysis using specific/phospho/total antibodies
Obtain the confirmation of putative protein using Mass Spectroscopy
2. Research Project “Analysis of key factors involved in cancer development by molecular and cell
biology techniques”
Preparation of high grade cDNA construct for the study of PAK2 function in breast cancer
cells
1. Transformation of PAK2-p34/pCDN3.1 into bacterial cells (DH5α)
2. Bacterial culture (mini and large scale)
3. Purification of Plasmid DNA by midi-prep kit from Qiagen
4. DNA Quantitation by UV absorbance method and DNA qualification by Agarose gel
electrophoresis
Analysis of PAK2 functions in mammalian cells
1. Culture mammalian cells (3T3-L1, MCF7, T47D, MDA-231, HEK293T)-Passing and sub-
culturing of adherent and non-adherent cells
2. Serum starvation study of cell lines (3T3L-1, MCF-7) and analysis of PAK2 activation
3. Prepare cell lysate and analyze endogenous PAK2 by WB from membranous fraction vs.
total cell lysate
4. Transfect PAK2-p34 into MCF7 and analyze its expression
3. Various other biotechniques
Subclone TSP-1(Thrombospondin-1) into mammalian expression vector and amplification by PCR
Glycosylation and silver stain study of APF (Anti-proliferative factor) to analyze sialoglyco motif on
small peptide
Measurement of protein concentration using Bradford assay
Immunoprecipitation of eIFs from prostate cancer patients’ urine samples
Caspase cleavage and analysis of proteins using phospho-specific Antibody
Bacterial cell culture, isolation of plasmid/DNA, quantification and amplification
Sanofi Pasteur Swiftwater, PA
Aseptic Technician II February, 2011-December, 2011
Filling and formulation under sterile grade A environment
Routine aseptic testing, environmental monitoring, filling vaccines under controlled environment,
CIP/SIP (cleaning of filling lines/sterilization of filling lines) and microbial plating for QC prior and
after aseptic manipulation.
Fully compliance with SOP, cGMP, BFRs (Batch filling records), federal regulation and regulatory
guidelines
Active engagement in training modules including revising and certification
References:
1. Pamela Norton, PhD. The Drexel University of Medicine, Doylestown, PA.
******.******@*********.***
2. Anand Mehta, PhD. The Drexel University of Medicine, Doylestown, PA.
*****.*****@*********.***
3. Jun Ling, PhD. The commonwealth Medical College, Scranton, PA. *****@******.***
4. Sue Bentler. Supervisor at Sanofi Pasteur, Swiftwater, PA. 570-***-****
***.*******@*************.***
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