Control Research
Resume:
Rajesh T. Patel, PhD
Madison, NJ 07940
*********@*****.***
PROFESSIONAL SUMMARY
Dedicated, passionate and results oriented scientist with strong background working with in vivo and in vitro
model systems; Exceptional scientific knowledge of obesity and type 2 diabetes animal models and research
expertise in basic and translational research including bariatric surgery. Highly skilled in methodology and
application of cell based in vitro functional assays and signal transduction. Through knowledge in
mechanisms that regulate body weight, insulin sensitivity and hepatic glucose production; experience
coordinating study activities with cross-functional teams and managing multiple projects. Strong track record
of publications including review articles and book chapters.
RESEARCH EXPERIENCE
Feb 2010 – Present
Weill Cornell Medical College
Research Associate, Laboratory of Timothy McGraw, PhD and Francesco Rubino, MD
Translational Research
Collaborated with pharmaceutical sponsor to investigate the molecular mechanisms involved in the body
weight regulation and improvement in glucose homeostasis after bariatric surgery in ZDF ra ts.
Optimized novel protocol to examine Insulin activity in plasma using single cell based fluorescent assay
in adipocytes
Current efforts are to Identify and characterize biomarkers for the pathophysiology of type II diabetes
using human and rodent plasma samples before and after bariatric surgery using combination of cell
based function assay, FPLC and mass spectrometry methodology. Pharmacological and molecular
biology approaches are used to characterize lead candidate proteins as novel drug targets
Characterized GIP and GPR120 receptors and its naturally occurring variants in adipocytes
April 2007 – Jan 2010
University of Pennsylvania School of Medicine, Philadelphia, PA
Postdoctoral Research, Laboratory of Dr. Rexford S. Ahima, M.D, PhD
Investigated the relationship between hepatic steatosis and insulin resistance by manipulating the
hepatic lipid levels in mice by either overexpression (using adenovirus) or down regulation (using
antisense oligonucleotide) of liver specific adipose differentiation related protein.
Using hyperinsulinemic-euglycemic clamp technique and other metabolic pheotyping studies our data
showed that hepatic steatosis per se does not cause hepatic insulin resistance in the a bsence of
lipogenic diet and systemic circulating factors. In contrast, our studies showed that reduction of hepatic
steatosis in high fat fed mice showed metabolic improvement in the presence of obesity.
Primary hepatocytes were used to investigate the m olecular mechanisms relating hepatic steatosis and
Insulin resistance.
EDUCATION
Oklahoma State University March 2007
Ph.D., Biochemistry and Molecular Biology
Laboratory of Drs. Jose L. Soulages and Estela L. Arrese,
Thesis: Mechanism of activation of lipolysis in adipocytes
Investigated the downstream signaling cascade in the stimulation of lipolysis using purified in vivo radio-
labeled natural lipid droplets, TG lipase and protein kinase A. Our group was the first to recapitulate
lipolysis in vitro using minimum lipolysis machinery without addition of any other cytosolic component.
The phosphorylation of 42 kDa lipid storage droplet protein 1 (LSDp1), the major phosphoprotein on the
surface of lipid droplets plays an important role in the stimulation of lipolysis.
Using enzymatic and inhibitor studies, we identified that TG lipase also has phospholipase A2 activity,
which is important for hydrolyzing phospholipid monolayer on the surface of lipid droplet during lipolysis.
Rajesh T. Patel, PhD
Maharaja Sayajirao University, Gujarat, India
M. Sc. Biochemistry May 2000
Thesis: Effects of dietary variation in omega-6/omega-3 fatty acid ratio on rat brain cell membrane fatty acid
composition and associated behavioral changes.
Brain cell membrane fatty acid composition varies with dietary variation in essential fatty acids.
Higher intake of omega-3 replaces omega-6 incorporation, associated with reduction in hyperactivity
(ambulatory test) and depression (swim test).
Gujarat University, Gujarat, India
B. Sc. Biochemistry May 1998
COMPUTER SKILLS
Microsoft office, Adobe acrobat (document creation and editing), Graph Pad statistical software,
GenBank, BLAST and FASTA.
RESEARCH EXPERIENCE/SKILLS
In vivo models: Mouse, Rat and Insect
In vitro models: Primary adipocytes and hepatocytes, AML-12 and 3T3 L-1 cells.
Mouse breeding schematics and genotyping, IP, SQ and tail vein injections
Hyperinsulinemic-euglycemic clamp
Surgeries: Intracerebral ventricular (ICV) and intestinal cannulations
Metabolic phenotyping using comprehensive laboratory animal monitoring system.
OGTT, ITT and D-xylose absorption test.
In vivo and ex-vivo fatty acid oxidations and uptake using radio labeled tracers
Immunohistochemistry / immunofluoroscense and histopathology
Adenoviral mediated gene delivery and antisense oligonucleotide and siRNA mediated gene knock -down
Serum chemistry and peptide hormones measurement using single and multiplex ELISA
In vivo rate of VLDL secretion assay and lipoproteins fractionation.
Protein purifications from native and recombinant cells using HPLC, FPLC, anion and cation exchange
and gel filtration chromatography.
Enzyme activity and inhibitor studies using radio-labeled ( H, C and P) compounds
3 14 32
1D and 2D gel electrophoresis, RTPCR and reporter constructs
Fluorescent microscopy and flow cytometry
Molecular biology: Prepare constructs, sequence verification, DNA and RNA preparation, cloning and
characterization of proteins using pharmacological and molecular approaches.
AWARDS
2010 Travel award: Neuronal Control of Appetite, Metabolism and Weight meeting
2008 Best poster presentation award: American College of Nutrition's 49th annual meeting
2007 Travel award: Peripheral and Central Pathways Regulating Energy Homeostasis meeting
2006 Travel award: Experimental Biology meeting
EXTRACURRICULAR & VOLUNTEER ACTIVITIES
Supervised peers, mentored master and undergraduate level students and technic al staff
Participated in planning and execution of strategies for the American Diabetes Association’s (ADA)
Northeast Philadelphia office (2008-2009)
Planned and executed graduate students research day for three years (2003 -2006)
Vice president and treasurer of Biochemistry Graduate Student Association (2003-2006)
W orked with World Food Program as a liaison after devastating earthquake in Gujarat, India (2001)
Rajesh T. Patel, PhD
PUBLICATIONS
*Mohammad S., *Patel, RT., *Bruno J, Wen J, Panhwar, MS., McGraw TE (2014). A naturally occurring GIP
receptor variant undergoes enhanced agonist-induced desensitization, which impairs GIP control of adipose
insulin sensitivity (Under review). * These authors contributed equally
Yore, MM., Syed, IS., Zhang, T., Moraes-Vieira, P., Homan, E., Herman, MA., Patel, RT., Lee,J.,
Dhaneshwar, Peroni, A., Hammarstedt, A., Ulf Smith, Tian, D., Greka, A., McGraw, TE., Saghatelian. A. and
Kahn BB. (2014) Novel Class of Endogenous Lipids that have Anti-Diabetic and Anti-inflammatory Effects
(submitted to Cell)
Shukla A., Patel, RT. and Rubino FR (2014) Diabetes Surgery (Book chapter, in press)
Patel, RT., Shukla, A, Moreira, M. and Rubino FR. (2014) Surgical control of obesity and diabetes: The role
of intestinal vs. gastric mechanisms in the regulation of body weight and glucose homeostasis . Obesity Jan;
22(1):159-69.
Sun Z., Miller, AR., Patel, RT., Chen, J., Dhir, R., W ang, H., Zhang, D., Graham, MJ., Unterman, TG.,
Shulman, GI., Sztalryd, C., Bennett, MJ., Ahima, RS., Birnbaum, MJ., and Lazar, MA. (2012). Hepatic
HDAC3 Promotes Gluconeogenesis by Repressing Lipid Synthesis and Sequestration. Nature Medicine.
Jun;18(6):934-42
*Carr, RM., *Patel, RT, Rao, V., Dhir, R., Graham, M. J., Crooke, RM. and Ahima, RS. (2012). Reduction of
TIP47 improves hepatic steatosis and glucose homeostasis in mice. AJP-Regulatory, Comparative and
Integrative Physiology. 302 (8) R996-R1003 *These authors contributed equally
Shukla A, Ahn Soo Min., Patel, RT., Rosembaum, M.W ., and Rubino, F.R. (2011) Surgical Treatment of
Type-2 Diabetes-The Surgeon Perspective. Endocrine. Oct; 40(2): 151-61
Arrese, E.L., Howard, A.D., Patel, RT., Rimoldi, O.J. and Soulages, J.L. (2010) Mobilization of lipid stores in
Manduca Sexta: cDNA cloning and developmental expression of the main fat body triglyceride -lipase, TGL.
Insect Biochemistry and Molecular Biology. 40(2), 91-99
Patel RT. and Ahima R. S. (2008). How do intestinal lipids control glucose homeostasis? Cellscience
reviews. Vol 5 (1) ISSN 1742-8130
Singal, N. S., Patel, RT., Qi, Y., Lee, Yun-Sik. And Ahima, R. S. (2008) Loss of resistin ameliorates hyper-
lipidemia and hepatic steatosis in leptin deficient mice. AJP-Endo and Metabolism. 295, E331-E338
Arrese, E.L., Patel, RT., and Soulages J.L. (2006). The main triglyceride-lipase from the insect fat body is an
active phospholipase A1: Identification and functional characterization of the phospholipase activity. J Lipid
Res. 47, 2656-67
Patel, RT., Soulages, J.L., and Arrese, E.L. (2006). Adipokinetic hormone-induced mobilization of fat body
triglyceride stores in Manduca sexta: role of TG-lipase and lipid droplets. Archives of Insect Biochem and
Physiology. 63, 73-81
Patel, RT., Soulages, J.L., Hariharasundaram, B., and Arrese, E.L. (2005). Activation of the lipid droplet
controls the rate of lipolysis of triglycerides in the insect fat body. J Biol Chem. 280, 226**-*****
Patel, R., Soulages, J.L., Wells, M.A., and Arrese, E.L. (2004). cAMP-dependent Protein Kinase of Manduca
sexta Phosphorylates but Does Not Activate the Fat Body TG Lipase. Ins Bio Mol. Biol. 1269-1279
M ANUSCRIPTS IN PREPARATION
Rajesh T. Patel, PhD
Patel, RT., Shukla, A, Ahn SM., Moreira, M. and Rubino FR. Regulation of Insulin, GLP-1 and Glucagon
response to acute and selective nutrient stimulation of distinct segments of gastrointestinal tract “Implications
for understanding mechanisms of bariatric and metabolic surgery” (2014)
Mohammad S., Patel, RT., Gonzalez, E., Richie, P., Rubino, FR and McGraw, TE. Diabetic Plas ma Acutely
and Actively Induces Insulin Resistance in Adipocytes, an Effect Reversed By Bariatric Surgery. (2014 )
Presentations in Conferences/meetings:
Invited speaker: New York Lipid and Vascular Biology Club Meeting - March 4, 2014
Title: Incretin GIP receptor activity in adipocytes
Abstracts
W ynne, N., Patel, RT. (presenter), Shukla, A., Moreira, M., Ahn, SM., Rubino, F (2012). Manipulations of
gastrointestinal anatomy reveal physiologic mechanism of GLP-1 regulation. American Diabetes Association,
Philadelphia, PA
Carr, RM., Patel, R.T, Rao, V., Dhir, R., Graham, M. J., Crooke, RM. and Ahima, RS. (2011). Reduction of
TIP47 improves hepatic steatosis and glucose homeostasis in mice. Poster presentation at the American
Association for the study of Liver disease, San Francisco, CA.
Ahn, Soo Min., Patel, R.T., Moreira, M., Wynne, N., Shulka, A.P., and Rubino F. (2011) Duodeno-Jejunal
Bypass Prevents Progressive Decline in Insulin levels in Zucker Diabetic Fatty Rats by a W eight-
nd
Independent Mechanism: Poster at 2 World Congress on Interventional Therapies for Type-2 Diabetes.
Ahn, Soo Min., Patel, R.T., Shukla, A.P., Moreira, M., Wynne, N., and Rubino, F. (2011) . The effect of DJB
nd
on Glucose tolerance in Zucker Diabetic Fatty Rats: A Time Course Study: Poster Presentation at 2 World
Congress on Interventional Therapies for Type-2 Diabetes.
Patel, R. T., Dhir, R., Yin, X. and Ahima R. S. (2010). Overexpression of Adipose Differentiation-Related
Protein induces hepatic steatosis and enhances insulin sensitivity in muscle : Poster presentation at the
Neuronal Control of Appetite, Metabolism and Weight, Keystone; Colorado
Saiyed, Z. M, Patel, R.T., Telang, S. D., Rao, K., Thangavel, S., Kadiyalababu, D., Gandhi, N., Nair M.
(2008). Effect of Dietary Variation in n-6/n-3 Ratio on Rat Brain Membrane Fatty Acid Composition and
Associated Behavior: Poster at the American College of Nutrition's 49th Annual meeting, Arlington, Virginia
Patel, R.T., Soulages, J. L., Arrese, E.L. (2007) Mobilization of fat body triglyceride stores in Manduca sexta:
role of TG-lipase and lipid droplets. Poster presentation at the Obesity: Peripheral and Central Pathways
Regulating Energy Homeostasis meeting, Colorado.
Patel, R.T., Soulages, J. L., Arrese, E.L. (2006) Activation of the lipid droplet controls the rate of lipolysis of
triglycerides in the insect fat body: a role for lipid storage droplet protein 1. Poster presentation at the
Experimental Biology meeting, San Francisco. The FASEB Journal, 20, Abstract # 90.23
Arrese, E.L., Patel, R.T., and Soulages, J. L. (2006) Mobilization of fat body triglyceride stores in Manduca
sexta: role of TG-lipase and lipid droplets. Poster presentation at the 5th International Symposium on
Molecular Insect Science, Tucson, Arizona.
Patel, R.T., Soulages, J. L., and Arrese, E.L. (2006) Identification and functional characterization of the main
triglyceride lipase from the fat body of adult Manduca sexta. Poster presentation at the 5th International
Symposium on Molecular Insect Science, Tucson, Arizona.
Patel, R.T., Soulages, J. L., and Arrese, E.L. (2005). Activation of the lipid droplet controls the rate of
lipolysis of triglycerides in the insect fat body: a role of lipid storage droplet protein 1. Poster presentation at
th
the 16 Annual Research Symposium, Oklahoma State University.
Rajesh T. Patel, PhD
Smith A, Patel, R. T., Chetty, P., Soulages, J. L. and Arrese, E. L (2005) Mobilization of triglyceride stores in
th
the insect fat body-effect of calcium and cAMP on the activation of lipolysis. Poster presentation at the 16
Annual Research Symposium, Oklahoma State University.
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