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Jared Francis Stefanick Notre Dame, IN 46556

Email: acec50@r.postjobfree.com Phone: 850-***-****

Summary of Qualifications

Four years experimentation work experience with formulation of advanced drug delivery systems including ligand-

targeted nanoparticles (liposomes and micelles) and development of cyclic antibody delivery systems for improved

cancer therapy

Two years experience with development of novel purification methods fo r both liposomes and antibodies

Experience with extensive biophysical characterization, analytical methods, and chemical synthesis techniques

including: ELISA, ITC, FRET, PCR, SPR, AUC, DLS, MALDI-TOF, ESI-MS, HPLC, SEC,

light/fluorescence/confocal microscopy, flow cytometry, and solid phase peptide synthesis

Proficiency with aseptic cell culture and experimentation (cell passaging, media preparation, tissue culture procedures)

and aseptic technique with immunocompromised mice (handling, restraint, subcutaneous injections, euthanasia,

dissection)

Proficiency with the preparation of technical manuscripts and the assembly/analysis of large data sets

Education

University of Notre Dame: Notre Dame, Indiana

Ph.D. Chemical and Biomolecular Engineering: Ph.D Candidate Expected June 2014

M.S. Chemical and Biomolecular Engineering May 2012

Cumulative GPA: 3.667/4.000

Florida State University: Tallahassee, FL

B.S. Chemical Engineering: Concentration in Biomedical Engineering May 2009

Cumulative GPA: 3.879/4.000 (Magna Cum Laude)

President’s List- 3 terms, Dean’s list-3 terms

Engineering and Technical Experience

University of Notre Dame Chemical and Biomolecular Engineering Department : Notre Dame, Indiana

Graduate Student August 2009-Present

Rational Engineering of Designer Nanoparticles for the Selective Targeting of Tumors

Engineered VLA-4 and HER2 peptide-targeted micelles and liposomes to improve intracellular drug delivery

Dissected the roles of nanoparticle stealth coatings, peptide linker length, peptide affinity, and peptide biophysical

properties on cellular uptake of nanoparticles

Identified, fabricated, purified, and characterized targeting ligands for incorporation into nanoparticles

Evaluated in vitro cellular findings with multiple myeloma and breast cancer cells through flow cytometry

Performed in vivo biodistribution, toxicity, and tumor growth inhibition studies using SCID and nude mice

Enhanced Selectivity of Antibody Therapeutics to Cancer Cells

Designed antibody complexes to improve tumor targeting by reducing non -selective, normal cell targeting

Examined stability and selectivity of antibody complexes through extensive biophysical characterization including:

DLS, SEC, FRET, ELISA, ITC, and SPR

Purification of Therapeutics

Assisted in the development of experimental techniques for the salt -induced precipitation of pharmacologically

relevant monoclonal antibodies (Herceptin/Rituxan/Erbitux) and functionalized liposomes

Verified integrity of purified biological molecules using flow cytometry

University of Notre Dame Chemical and Biomolecular Engineering Department : Notre Dame, Indiana

Graduate Teaching Assistant August 2009 -May 2011

Chemical Reaction Engineering, Biomolecular Engineering Lab, Topics in Biomolecular Engineering, Chemical

Engineering Laboratory I

Responsibilities included: hosting weekly counseling sessions, evaluating and grading student assignments, designing

and validating new experiments, and ensuring adherence to proper experimental and safety protocols

Kaneb Center Outstanding Graduate Student Teacher Award, Spring 2011

Skills Developed: mentoring, curriculum development, evaluation, managing, interpersonal skills, flexibility, creativity

Publications

Noble GT, Stefanick JF, Ashley JD, Kiziltepe T, and Bilgicer B. Ligand -Targeted Liposome Design: Challenges and

Fundamental Considerations. Trends in Biotechnology 2014, 32: 32-45.

Xiao H, Noble GT, Stefanick JF, Ruogu Q, Kiziltepe T, Jing X, and Bilgicer B. Photosensitive Pt(IV)-azide prodrug-loaded

nanoparticles exhibit controlled drug release and enhanced efficacy in vivo. Journal of Controlled Release 2014, 173: 11-17.

Stefanick JF, Ashley JD, and Bilgicer B. Enhanced Cellular Uptake of Peptide-Targeted Nanoparticles through Increased

Peptide Hydrophilicity and Optimized Ethylene Glycol Pep tide-Linker Length. ACS Nano 2013, 7: 8115-8127.

Alves NJ, Cusick W, Stefanick JF, Ashley JD, Handlogten MW, and Bilgicer B. Functionalized liposome purification via

Liposome Extruder Purification (LEP). Analyst 2013, 138: 4746-4751.

Handlogten MW, Stefanick JF, Alves NJ, and Bilgicer, B. Nonchromatographic Affinity Precipitation Method for the

Purification of Bivalently Active Pharmaceutical Antibodies from Biological Fluids. Anal. Chem. 2013; 85: 5271-5278.

Alves NJ, Champion MM, Stefanick JF, Handlogten MW, Moustakas DT, Shi Y, Shaw BF, Navari RM, Kiziltepe T, and

Bilgicer, B. Selective Photocrosslinking of Functional Ligands to Antibodies via the Conserved Nucleotide Binding Site.

Biomaterials, 2013, 34: 5700-5710.

Xiao H, Stefanick JF, Jia X, Jing X, Kiziltepe T, Zhang Y, and Bilgicer B. Micellar Nanoparticle Formation via Electrostatic

Interactions for Delivering Multinuclear Platinum (II) Drugs. ChemmComm, 2013, 49: 4809-4811.

Stefanick JF, Ashley JD, Kiziltepe T, and Bilgicer B. A Systematic Analysis of Peptide Linker Length and Liposomal

Polyethylene Glycol Coating on Cellular Uptake of Peptide -Targeted Liposomes. ACS Nano 2013, 7: 2935-2947.

Stefanick JF, Kiziltepe T, Handlogten MW, Alves NJ, Bilgicer B. Enhancement of Antibody Selectivity via Bicyclic

Complex Formation. Journal of Physical Chemistry Letters, 2012, 3: 598-602

Kiziltepe T, Ashley JD, Stefanick JF, Qi YM, Alves NJ, Handlogten MW, Suckow M, Navari R, Bilgicer B. Rationally

Engineered Nanoparticles Target Multiple Myeloma Cells, Overcome Cell Adhesion-mediated Drug Resistance, and Show

Enhanced Efficacy. Blood Cancer Journal, 2012, April; 2: e64

Presentations

Enhancement of Antibody Selectivity via Bicyclic Complex Formation

o Poster: Advanced Diagnostics and Therapeutics Symposium (July 24, 2012), Notre Dame, IN.

o Poster: Advanced Diagnostics and Therapeutics External Review (September 24, 2012), Notre Dame, IN

Rationally Engineered Nanoparticles for Overcoming Drug Resistance in Multiple Myeloma

o Talk: AIChE Annual Meeting (October 28-November 2, 2012), Pittsburgh, PA

Analysis of Peptide Linker Length and Liposomal PEG Coating on Cellular Uptake of Targeted Lip osomes

o Poster: Harper Cancer Research Institute Research Day (April 15, 2013), Notre Dame, IN

o Talk: AIChE Annual Meeting (November 3-November 8, 2013), San Francisco, CA

Graduate Coursework

Mathematical Methods Engineering Engineering Applications of Medical Physiology

Advanced Chemical Engineering Thermodynamics Topics in Bimolecular Engineering

Transport Phenomena Biochemical Equilibria

Advanced Chemical Reaction Engineering Protein Structure and Folding

Biomedical Engineering Transport Phenomenon Biochemical Methods

Tissue Engineering Bio-organic Chemistry

Molecular Pharmacology

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