Engineering Assistant

Linxia Zhang

Email: ***********@*****.*** Phone: 517-***-****

Address : ** **** *****, *********, **, 12309 Status: Green Card

SUMMARY OF QUALIFICATIONS

Extensive experience with in vitro cell culture (stem cells, primary neural cells and other

transformed cell lines), co-culture and 3D culture models.

Strong molecular and cellular biology technical skills, such as cell based assays,

immunostaining, microscopy, RT-PCR, western blotting, flow cytometry and ELISA.

Proficient in array data analysis and statistical analysis.

Excellent scientific writing, communication, presentation and team work skills.

EDUCATION

08/2004-08/2010 Michigan State University, East Lansing, MI

Ph.D, Biochemistry & Molecular Biology, Materials Science and Engineering

09/2000-06/2004 Fudan University, Shanghai, China

BS, Polymer Science and Engineering

PROFESSIONAL EXPERIENCES

05/2012-Present Postdoctoral Research Associate

Biomedical Engineering, Rensselaer Polytechnic Institute

Accelerated the understanding of how electrical stimulation may be beneficial for repair of

peripheral nerve injury and spinal cord injury.

Addressed the role of matrix metalloproteinase (MMP) in regulating cell migration in 3D

biomaterials in the presence of electrical fields.

09/2010-04/2012 Postdoctoral Research Associate

School of Pharmacy, University of Wisconsin-Madison

Demonstrated the importance of antioxidative gene Nrf2 in recovery from peripheral nerve

injury in mice models.

Evaluated the role of antioxidative gene Nrf2 in mice models of neurodegenerative

diseases such as Parkinson’s, Alzheimer’s and ALS disease.

01/2005-08/2010 Research Assistant

Chemical Engineering and Materials Science, Michigan State University

Investigated the effect of cAMP on mesenchymal stem cells (MSCs) differentiation and

morphological changes.

Uncovered the role of CREB in modulating calcium signaling in MSCs.

Addressed the toxicity of fatty acids on human hepatocellular carcinoma cells (HepG2).

06/2003-07/2004 Research Assistant

Polymer Science and Engineering, Fudan University, China

Explored the complex phase separation behavior in polyethersulpone and epoxy blends.

Evaluated mechanical properties (such as tensile strength, toughness and elasticity) for

various polymers.

SELECTED PUBLICATIONS

Zhang, L. and Thompson, DM. AC pre-stimulated Schwann cells enhance NGF secretion

and promote neurite outgrowth in DRG neurons (To submit).

Zhang, L., Liu, L, Thompson, R. and Chan, C. CREB modulates calcium signaling in cAMP

induced bone marrow stromal cells (Accepted by Cell Calcium).

Zhang, L., Johnson, DA and Johnson, JA. Deletion of Nrf2 impairs functional recovery, slows

clearance of myelin debris and delays axonal remyelination after peripheral nerve injury.

Neurobiol Dis. (2013) 54: 329-338.

1 1 1

Cho, H., Wu, M., Zhang, L., Thompson, R., Nath, A. and Chan, C. Signaling dynamics of

palmitate-induced ER stress responses mediated by ATF4 in HepG2 cells. BMC Syst Biol.

1

(2013) 7: 9. These authors contributed equally to this work.

Zhang, L., Seitz, L., Abramczyk, A., Li, L. and Chan, C. cAMP initiates early phase

neuron-like morphology changes and late phase neural differentiation in mesenchymal stem

cells. Cell Mol Life Sci. (2011) 68 (5), 863-876.

Zhang, L. and Chan, C. Isolation and enrichment of rat mesenchymal stem cells (MSCs) and

separation of single-colony derived MSCs. J Vis Exp. 2010 Mar 22;(37). pii: 1852. doi:

10.3791/1852.

Zhang, L., Seitz, L., Abramczyk, A. and Chan, C. Synergistic effect of cAMP and palmitate in

promoting altered mitochondria function and cell death in HepG2 cells. Exp Cell Res. (2010)

316(5):716-727.

Srivastava, S., Zhang, L., Jin, R., and Chan, C. (2008) A Novel Method Incorporating Gene

Ontology Information for Unsupervised Classification and Feature Selection. PLoS ONE

3(12), e3860 (p.1-8).

Tang, X., Zhang, L., Wang, T., Yu, Y., Gan, W. and Li, S. (2004) Hydrodynamic Effect on

Secondary Phase Separation in an Epoxy Resin Modified with Polyethersulfone.

Macromolecular Rapid Communications 25(15), 1419-1424.

SELECTED CONFERENCE PRESENTATIONS

Zhang, L., Koppes, AN. and Thompson, DM. AC stimulated Schwann cells increase NGF

secretion and promote greater neurite outgrowth. 2013 BMES (Biomedical Engineering

Society) Annual Meeting.

Zhang, L., Seitz, L., Abramczyk, A. and Chan, C. Time-dependent and shape-dependent

cytoskeleton reorganization in mesenchymal stem cells (MSCs) induced by cAMP. 2009

AIChE (American Institute of Chemical Engineers) Annual Meeting.

Zhang, L., Seitz, L. and Chan, C. Differential regulation of p21 and p27 in cAMP induced

differentiation and apoptosis in mesenchymal stem cells. 2008 AIChE Annual Meeting.

Zhang, L. and Chan, C. Cyclic adenosine monophosphate signaling caused differentiation

and apoptosis in mesenchymal stem cells. 2007 AIChE Annual Meeting.

Zhang, L. and Chan, C. cAMP induced neural differentiation of mesenchymal stem cells: real

differentiation or pseudo-differentiation. 2007 ACS (American Chemical Society) National

Meeting & Exposition.

HONORS

Michigan State University dissertation completion fellowship (2010), Graduate School summer

fellowship (2008), and QBMI (Quantitative Biology & Modeling Initiative-MSU) fellowship (2006).

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