Maintenance Assistant

Andrew Tomaszewski

**** ******* ****** ************ ** 19106

Cell: 770-***-**** Email: *********************@*****.***

Education

Rutgers University,

Masters of Science Microbiology 2013

Georgia College and State University,

Bachelors of Science Chemistry 2009

Relevant Work Experience

Janssen Pharmaceuticals Jan 2014 July2014

Protein Purification Scientist I

Focused on protein purification, for downstream PDMS Large Molecule Group under ICH guidelines

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and regulations.

Developed, and carried out, Validation Study analyzing various purification modifications and its effect

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on yields

Worked in cohesion with international manufacturing scale lab, to develop small scale representative

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experiments of specific viral protease inhibitors

Assisted in upstream, Bioreactor maintenance, and large scale cellular growth

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Drexel College of Medicine May 2012 Jan 2014

Research Assistant (BioChemistry)

Investigated the RNA Splicesome complex, and its protein binding interactions in order to better

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understand its mechanism and function.

Responsible for writing new Lab Protocols and Optimizations along with maintain organization of these

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files in E Journal format

Proficient in Ligation Independent Cloning, high throughput cloning, RNA cloning

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Vast experience in Size Exclusion Protein Purification and isolation

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Trained in HPLC and FPLC techniques, Protein Crystallography, Enzymatic Assays, RNA Assays, SDS

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Page Gels, buffer preparation, Bradford Reagent testing, Nano drop

Proficient in cell culturing technique, growth and maintenance and knockdown of HeLa and P98 cell

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lines, hemocytometer use and hood use.

Emory Human Genetics Lab May 2009 Jan 2011

Lab Technician

Worked in multiple labs performing patient sample acquisition, DNA processing, Western Blot, and

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Immuno fluorescent Arrays

Organized DNA sequence results in their proper gene code, replacing batch numbers on mislabeled

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documents, following strict OSHA standards

Partnered with the Bioinformatics department to view mutations across multiple Genetic disorders

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examining patterns of dysfunctional sequences, Fragile X syndrome

Kennesaw State University May 2010 Aug 2010

Research Assistant

Examined ecological factors of waste dumping of plants in the gulf coast during the BP Oil Spill, and

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established standards of tolerance amongst various plant grass species.

Processed nutrient samples, (PO4, NH4), Carbon to Nitrogen ratio, Spectrometer analysis of water

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samples. Created standardized measurements of tolerance based off this data.

Lab Skills: Size Exclusion Protein Purification using Bio Rad NGC and GE ATKA AVANT, Gel Filtration, Sitting Drop

Hanging Drop Crystallography, Chromatography, SDS Page gels, Western Blot, Gel Imaging, Plasmid Preparation,

Restriction Enzyme Digestion, Sonication, Nanodrop Spectrometer Analysis, DNA RNA Amplification and Extraction,

Ligation Independent Cloning, Enzyme Assays, Aseptic Cell Culture Propagation and Maintenance using HeLa and P98,

Buffer and Media preparation, Confocal Microscopy, Large Scale Bioreactor maintenance, GLP, GMP

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