Manager Project

SHIRLEY SHPUNGIN e-mail: ********@******.***

*** ***** *** ******** ** 19440 tel: 215-***-****

EDUCATION

Temple University, Philadelphia PA

September 2009 - May 2013

BS: Biology Minor: Public Health GPA:

3.40

Temple University, Philadelphia, PA

September 2013 - May 2015

MS: Biotechnology GPA:

3.80

RESEARCH EXPERIENCE

Biology Co-op at GlaxoSmithKline, King of Prussia P.A July 2013 -

June 2014

. Working in the Respiratory R&D, specifically in the Stress and

Repair DPU. Currently I am conducting various experiments to

understand how ROS causes mitochondrial dysfunction and how it

pertains to respiratory diseases such as COPD.

. Plan weekly experiments around the team's objectives and

interests.

. Meet individually with my manager to talk about my current data

and my next experiments.

. Participate in project meetings biweekly and attend monthly

presentations on current research data.

. Write out thorough protocols, collect data, and organize

experiments in a notebook and provide analysis on results.

Skills and techniques: DNA and RNA isolation and analysis, qPCR using

Taqman and ViiA, rtPCR, cell tissue culture, Comet assay, Biochemical

assays, ELISA assays, high content imaging (Operetta), gel electrophoresis,

siRNA transfection, BacMam transduction, fluorescence staining, microscopy,

spectophotometery using Envision and plate readers, tissue

dissociation/cell isolation via GentleMACS, cell fractionation and

centrifugation, in vivo work with mice and rats.

Biology Intern at Temple University, S.H.R.O Aug.

2011 to May 2013

. Focused on Pin1 protein analysis through understanding the

oncogenic effect Pin1 has on other proteins by observing cell

proliferation in various cancer cell lines.

. Participated in project meetings and gave monthly presentations on

current research data.

. Collected data and organized experiments in a notebook and

provided analysis on results.

Skills and techniques: Western Blot, PCR, gel electrophoresis, SDS-PAGE,

fluorescence staining, photo development, cell tissue culture, microscopy,

UV and visible spectophotometry, cell fractionation, and genotyping.

ACTIVITIES/SKILLS

. Russian as a second language.

. Computer knowledge with Mac's and PC's using Microsoft Excel,

Works, PowerPoint, eLNB, GraphPad Prism 5, ViiA RUO 7, Harmony

. Founder of Owls for Israel.

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