Development Maintenance

ARCHNA GAUTAM

************@*****.*** 248-***-****

EXPERIENCE

‘12 –present BAVARIAN NORDIC INC. Mountain View, CA

Sr. Research Associate I (QC / R&D)

Experience with cell based bioassays used for QC release testing (FACS, Restriction Site Analysis and PCR)

Designed and performed experiments to characterize BNIT’s poxvirus based cancer immunotherapy

candidate’s product characterization.

Supported new CV-301 (Immunotherapy product candidate) construct generation by expression and genetic

stability studies.

Research Associate II (QC / R&D)

Determined titers and transgene expression for Fowlpox, Vaccinia and MVA viruses manufacturing lots.

Worked under GMP guidelines and Complied with QA-controlled SOPs and documentation procedures.

’09 -‘12 GERON CORPORATION Menlo Park, CA

Product Development Associate II

Co-ordinated planning and execution of GRNOPC1 (Oligodendrocyte progenitor cells) studies for

maintenance and directed differentiation of hESC (Human embryonic stem cell). Also, managed

experimental records related to improvement in production and manufacturing processes.

Provided strategic direction and technical expertise to develop and optimize GRNOPC1 drug development

process to increase efficiency and to reduce production costs.

Led data analysis (FlowJo) to test purity in drugs to comply with quality standards for development of

therapeutic products.

Developed a novel assay for accurate quantization of GRNOPC1 to improve overall step yiel d.

‘09 ROCHE PHARMACEUTICALS Palo Alto, CA

Research Associate I

Managed production of mammalian cell lines (including SW1353, HCT116, EBNA, C6/36, HEK -293,

CHO-DUK, BHK, MDCK II, Jurkat, Ramos, CHO-K1) used in testing drug efficacy.

Performed transfection, transduction and mycoplasma testing of cell lines. Also, involved with

cryopreservation, thawing and Immunostaining of cells for FACS.

Successfully handled robotic systems to automate 96 and 384 well-plates for various cell lines. Also,

maintained laboratory equipment and apparatus to improve their effectiveness and safety.

’05 -‘08 WAYNE STATE UNIVERSITY Detroit, MI

Research Associate

Involved in research to understand CTCF (CCCTC binding protein) as a key factor linked to epigenetics,

Breast cancer, and Wilm’s tumors. Expressed the CTCF protein in E.coli.

’02 - ‘03 CENTRAL SOIL SALINITY RESEARCH INSTITUTE (CSSRI) Karnal, India

Senior Research Fellow

Conducted research on development of agri-horticultural systems suited for bio-saline agriculture.

EDUCATION

WAYNE STATE UNIVERSITY Detroit, MI

o Masters of Science, Molecular Biotechnology, 2008

KURUKSHETRA UNIVERSITY Kurukshetra, India

o Masters of Science, Botany, Zoology, 2002

R&D TECHNIQUES / LAB-WORK

ES Cell Culture - Maintenance and Differentiation

Experience in molecular virology and virus infection techniques in cell culture

Flow Cytometry (FACS) and FlowJo analysis

Plasmid Isolation, PCR techniques, Primer design, BLAST, PDB, DNA/RNA extraction

Protein expression and purification, vector construction, molecular cloning, Immunology techniques,

Polyacrylamide Gel Electrophoresis, Western Blotting, In-situ hybridization

Immuno-blotting, Histological techniques, Restriction enzyme digestion, Purification and Characterization

of Antibodies

Advanced FACS training from BD Biosciences.

REFERENCES: Submitted on request

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