Engineer Manager

MANQIU CAO, M.S.

**** ******* *** **** *, Palo Alto, CA94306

Tel: 650-***-**** (c), 650-***-**** (H), e-mail: ***.******@*****.***

Objective: Looking for a research associate position in a biotech company

in conducting DNA sequencing or other molecular biology research

Skill Highlight

Cell and Molecular Biology

DNA extraction, PCR, qPCR, DNA enzymatic manipulation, DNA genotyping and

sequencing.

Gel electrophoresis, DNA cloning and cell transfection.

Maintaining cell lines, Conducting antibody conjugation and ELISA.

Immunohistochemistry (IHC) and In situ.

BioAssays

DNA sequencing and genotyping with genechip Assays.

DNA qPCR assay and PicoGreen assay.

ELISA and other Immuno staining assay.

Immunohistochemistry (IHC) and In situ assay.

Instrument and Computer

Conducted sequencing assay and using Illumina Hiseq and Miseq instrument.

Developed and performed automation of Affymetrix 96-well WGA assay for

genotyping, which including conduct automatic 96-well format WGA, DNA

fragment, Hybridization with gene chip, ligate adapter on chip, stain

with biotinylated antibody, stain with SAPE, and auto scan 96-chips.

Performed illumina cluster generation for sequencing on cBot system.

Performed Agilent HPLC 1100 series for synthesized oligo analyzing.

Skillful with Affymetrix and Illumine data analysis software.

Used Agilent BioAnalizer and qPCR instrument Stratagene Mx3000P daily.

Maintained large data sets with Excel.

Professional / Personal

Self-motivated, and detail oriented team player.

Eager to learn new techniques.

Capable of managing multiple complex projects.

Experienced in troubleshooting on experiments to understand root cause

and identify solutions.

Deliver daily and weekly report with PowerPoint on time.

Efficient in participates group meetings, presents results of work,

interprets data, and draws conclusions.

EXPERIENCE

09/2013-04/2014 Senior Research Associate, Intel Corporation, Santa

Clara

1. Develop next generation of Intel gene chip sequencing technology,

and improve DNA sequencing assay methods and reagents.

2. Conducted Agilent HPLC 1100 series for synthesized oligo analyzing.

3. Design and conduct experiments as well as weekly present and report

experimental results.

04/2010-08/2013 Senior Research Associate, Centrillion

BioScience Inc., Palo Alto, CA

1. Dedicated to develop faster and cheaper sequencing methods and

instruments for DNA library prep and reagents.

2. Took active role as lab manager for sequencing customers' DNA

samples with Illumina sequencing assay and instruments.

3. Run sequencing sample prep and assay in high throughput way.

4. Maintain appropriate inventory of lab supplies.

5. Log and tract customer sample through sequencing

6. Acted as wet Lab resource, independently planned, designed, and

conducted experiments to support research projects for customers.

Customers including: Gilead, Stanford University, Columbia

University, UCSF, John Hopkins, MGH, Colorado University, SVBio et

al.

7. Recruited and retained customers in generating >$2MM revenues.

03/2008-03/2010 Senior Research Associate, Affymetrix Inc., Santa

Clara, CA

Conducted key experiments for Affymetrix WGA assay protocol and Axiom

96-array automation product.

1. Conducted customer's DNA sample genotyping assay and trained

customers with AFFY's instruments.

2. Conducted SNP screening and genotyping system validation.

3. Developed and optimized protocols for high-throughput SNP

genotyping that resulted in three commercial products: 10K, 100K

and 500K products.

4. Designed and conducted key experiments leading to feasibility of

10K to 500K (e.g. increasing complexity tolerance of high density

genotyping chip)

10/2005-02/2008 RA, Digital Health BioMedical Group, Intel Corp.,

Santa Clara, CA

1. Developed and optimized protocols for composition of nano-particle

label reagents.

2. Designed and conducted key experiments in Bio-conjugation of nano-

particle with antibody leading to commercial products to support

collaborators using in cell analysis

08/2000-09/2005 Senior Research Associate, Affymetrix Inc.,

Santa Clara, CA

1. Work with collaborators from NIH, Manchester, AstraZeneca and John

Hopkins University to complete large-scale genetic association

studies in high quality but very aggressive deadline.

2. Experience in training and supervision: training temps and new

colleagues; supervision in big project of construction of Genetic

Map, 10K, 100K and 500K screening; analysis of large data sets and

maintenance of detailed records.

3. Optimized whole genome amplification method; designed and conducted

experiments in human repetitive sequences (ie Alu) depletion and

detection.

4. Took an active role in research and development for novel

technologies in highly multiplexed DNA and RNA target manipulation.

Resulted in several patents and publications.

06/1998-07/2000 Research Associate, Chiron Corporation,

Emeryville, CA

1. Conducted drug screening experiments on cell lines with

Luciferase assay and Western blot.

2. Optimized panning procedures for selection of antibodies from

phage libraries; Utilized ELISA, cloning and sequencing for

characterizing scFv antibodies; Conducted Western blot for

protein expression, Immunohistochemistry to detect protein

expression on human tissues and cancer cell line.

3. Established and optimized In situ hybridization protocol and

successfully detected over 50 cancer genes expression on

hundreds human cancer tissues and normal tissues. Skillful with

microdisectrion of FFPE tissue and using microscope for tissue

slides.

06/1997-05/1998 Research Associate, CVRI & Med, UCSF

1. Performed screening of cDNA libraries by 32 P labeled probe;

conduct cloning, PCR, plasmid construction.

2. Study death factors (drug) in human tumor cell lines. Conducted

Luciferase assay and Western blot.

10/1996-06/1997 Research Associate, Harvard Medical

School / MGHospital

1. Studied expression of tumor resistance gene (IL6) in cancer cell

lines, strains of mouse, and organs.

2. Extracted total RNA from tissue and cell lines; conducted RT.PCR,

cloning, Western blot.

PATENTS

1. Methods for Genotyping Ultra-High complexity DNA. Patent #

200********-**/23/04. Inventors: Manqiu Cao and Giulia Kennedy.

2. Use of guanine analogs in high-complexity genotyping. Patent #

200********. 04/07/05. Inventors: Giulia Kennedy, Robert Kuimelis,

Michael Savage, Manqiu Cao.

PUBLICATIONS

1) Composite Organic-Inorganic Nanoparticles as Raman labels for

tissue analysis. Nano Letters (Jan 2007) Lei Sun, ...Manqiu Cao,

el al. and, Beatrice Knudsen.

2) A whole genome long-range haplotype (WGLRH) test for detecting

imprints of positive selection in human populations.

Bioinformatics 22 (17): 2122-2128 (2006) Chun Zhang, ...Manqiu Cao

et al. and Giulia C. Kennedy

3) Oligonucleotide Microarray Analysis of Genomic Imbalance in

Children with Mental Retardation. J M Friedman Manqiu Cao, el

al, and Marco A Marra. Am J Hum Genet. 2006 Sep ;79:500-513

4) Oligonucleotide Microarray Analysis of Genomic Imbalance in

Children with Mental Retardation Howard R. Slater, Manqiu Cao et

al, and Giulia C Kennedy. The American Journal of Human Genetics,

volume 78(4) (2006), pages 730-731

5) A Genome-wide linkage analysis of Alcoholism on Microsatellites and

SNPs data, using alcohol dependence phenotypes and EEG measures

Chun Zhang, Simon Cawley...Manqiu Cao et al. and Giulia Kennedy.

MBC Genetic 2005, 6 (Suppl 1):S2

6) Whole-Genome Scan, in a Complex Disease, Using 11,245 single-

Nucleotide Polymorphisms: comparison with Micro satellites. Sally

John...Manqiu Cao et al. and Giulia Kennedy American Journal of

Human Genetics Vol. 75, No.1, 54-64 July 2004

7) Large- scale genotyping of complex DNA. Giulia C Kennedy, Hajime

Matsuzaki, Guoying Liu, Xing Su, Manqiu Cao el al, and Keith W.

Jones. Nature Biotechnology 21, (10) October 2003.

8) High-throughput generation and engineering of recombinant human

antibodies Barbara Krebs, Jill Winter... Manqiu Cao et al. and

Titus Kretzschmar Journal of Immunological Methods 254(200) 67-84

April 2001.

9) PCD1, a novel gene containing PDZ and LIM domains, is overexpressed

in several human cancers Sanmao Kang... Manqiu Cao et al. and

Giulia C. Kennedy Cancer Research 15;60(18):5296-302 Sep 2000.

10) Cloning and sequencing of bovine Ca2+-dependent adenosine

triphosphatase. Manqiu Cao and Dr. Donord C. Beitz, Iowa State

University Thesis, 1996.

EDUCATION

1996 M.S. in Molecular Biology, Iowa State University, Ames, Iowa

1985 B.S. in Chemical Engineer, East China University of Chemical

Technology, shanghai, China

REFERENCES AVAILABLE UPON REQUEST

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