assay development, molecular biology

Zhiqing (Sue) He, M.S.

**** ********* *****, ****** ******, NJ 07076

908-***-**** 908-***-****(cell)

*******@*****.***

SUMMARY

. A highly skilled and versatile laboratory scientist with excellent

skills in molecular biology, cell biology and protein biochemistry.

. Experienced in the areas of infectious diseases (Microbiology and

Virology) and signal transduction pathways (GPCRs and

hypertension).

. Proficient in development and validation of cell-based, biochemical

and enzymatic assays.

. Hands-on experience in expression and purification of recombinant

proteins.

. Proven track record of maintaining OSHA safety and SOPs compliance.

RESEARCH SKILLS

. Cloning, DNA, RNA Sequencing, site-directed mutagenesis, PCR, RT-

PCR(Taqman), SDS-PAGE, Western blotting, ELISA.

. Stable and transient transfection of CHO, Hek293 and Huh7 cells,

Cell Cultures.

. Operating various automated liquid handling systems (ECHO, Biomek

FX, Bravo, multidrop and Qiacube-HT) and multi-mode readers

(Envision Alpha).

. Assay development, automation and optimization: HTRF kinase assay,

luciferase assay, 3H/33P Binding assay (SPA and filtration), IP One

assay, HCV NS5B biochemical assay and HCV replicon assay.

. Viral de novo resistance selection studies; resistance mutation

genotypic and phenotypic analyses.

. Hits verification, compound library handling and data input and

analysis.

COMPUTER SKILLS

Proficient with Microsoft Word, Excel, PowerPoint, XLFit, Adobe

Photoshop,

Graph pad Prism, Activity Base, Vector NTI and DNASTAR.

COMPLIANCE & SAFETY TRAINING

Radiation Safety Orientation and annul refresh

Laboratory Chemical Hygiene, Laboratory Chemical Waste

Blood borne Pathogens, Laboratory Fume Hood

Personal Protective Equipment, Biosafety Awareness

OSHA Laboratory Standard, Good Laboratory Practices

WORK EXPERIENCE

Merck Research Laboratories, Kenilworth, NJ

2001- 2013

Sr. Scientist, In Vitro and Cellular Pharmacology

. Optimized cell based HTRF assays in 384-well format for high

throughput screens to evaluate target engagement of an interested

Kinase.

. Conducted mechanism of action studies to better understand

different sub-types of agonists of GPCR40. Assays included cell

growth, caspase activity, apoptosis (TUNEL), phosphorylation of

MAPK/ERK, phosphorylation of CREB and IP One assay.

Scientist/Sr. Scientist, Infectious Diseases

. Evaluated antiviral activity of HCV inhibitors targeting different

mechanisms (NS3 protease, NS5A, NS5B nucleoside and non-nucleoside,

replication inhibitors) in HCV replicon cells using luciferase

reporter and RT-PCR (Taqman) based readouts.

. Developed and optimized a novel HCV NS5B biochemical assay for high

throughput screen of HCV polymerase inhibitors; characterized HCV

replicase complex through biochemical fractionation.

. Conducted de novo resistance selection studies of NS5B nucleoside

and non-nucleoside inhibitor leads and in-licensed compounds;

performed genotypic and phenotypic analyses of selected resistant

mutant variants.

. Assessed synergy/antagonism of HCV drug combinations in both

biochemical assays and in replicon cells.

. Evaluated intracellular metabolism of nucleoside analogues and other

inhibitor leads in collaboration with Analytical Chemistry group.

Wyeth-Ayerst Research, Pearl River, NY

1994- 2001

Biologist I, Oncology/Infectious Diseases

. Characterized retinoic acid receptor isoforms and implemented a

compound screen for receptor ligands. Techniques included yeast

transformation, total protein extraction, band-shift assay, ?-Gal

assay, and hormone binding assay.

. Produced viral proteins through an E.coli expression system.

Techniques included purification of expressed proteins by affinity

chromatography and size exclusive chromatography.

. Adapted a yeast two-hybrid system to investigate the processes

involved in assembly of human respiratory syncytial virus and human

cytomegalovirus. Techniques included screening and cloning by PCR,

DNA sequencing and analysis.

SUNY Health Science Center at Syracuse, Syracuse, NY

1992- 1994

Department of Molecular Biology and Biochemistry

Research Assistant

Fudan University, Shanghai, China 1990-

1992

Institute of Genetics

Research Associate

EDUCATION

B.S. Bioengineering and Genetics

Fudan University, Shanghai, China

M.S. Molecular Biology and Genetics

Institute of Genetics, Fudan University, Shanghai, China

PUBLICATIONS

1. Huang HC, Ferrari E, He Z, Lesburg CA, Mirza U, McNemar C, Ba L, Tong

X, Ralston R, Chen KX, Venkatraman S, Hesk D, Njoroge FG, Kozlowski

JA, Rosenblum SB. Discovery and characterization of novel irreversible

HCV NS5B polymerase inhibitors. Global Antiviral Journal 2011, 7

(Suppl 1): 78.

2. Anilkumar GN, Lesburg CA, Selyutin O, Rosenblum SB, Zeng Q, Jiang Y,

Chan TY, Pu H, Vaccaro H, Wang L, Bennett F, Chen KX, Duca J, Gavalas

S, Huang Y, Pinto P, Sannigrahi M, Velazquez F, Venkatraman S,

Vibulbhan B, Agrawal S, Butkiewicz N, Feld B, Ferrari E, He Z, Jiang

CK, Palermo RE, McMonagle P, Huang HC, Shih NY, Njoroge G, and

Kozlowski JA. Novel HCV NS5B polymerase inhibitors: discovery of

indole 2-carboxylic acids with C3-heterocycles. Bioorg Med Chem Lett

2011, 21(18): 5336-5341.

3. Cheng CC, Huang X, Shipps GW, Wang Y, Wyss DF, Soucy KA, Jiang C,

Agrawal S, Ferrari E, He Z, and Huang HC. Pyridine carboxamides:

potent palm site inhibitors of HCV NS5B polymerase. ACS Med Chem Lett

2010, 1(9): 466-471.

4. Ferrari E, He Z, Palermo RE, Huang HC. Hepatitis C virus NS5B

polymerase exhibits distinct nucleotide requirements for initiation

and elongation. J Biol Chem 2008, 283: 338**-*****.

5. Salerno AJ, He Z, Goos-Nilsson A, Ahola H and Mak P. Differential

transcriptional regulation of the apoAI gene by retinoic acid receptor

homo and heterodimers in yeast. Nucleic Acids Research 1996, 24(4):

566-572.

6. He Z, Mao YM, Sheng RQ, and Sheng ZJ. Complete Nucleotide Sequence of

pheB gene encoding catechol 2,3-dioxygenase in B. stearothermophilus

FUTP-3. Chinese Biochemical Journal 1995, 11: 114-116.

7. Xu K, He ZQ, Mao YM, and Sheng RQ. On two transposable elements from

Bacillus stearothermophilus. Plasmid 1993, 29(1): 1-9.

8. Dong FM, Wang LL, Wang CM, Cheng JP, He ZQ, Sheng ZJ, Sheng RQ.

Molecular cloning and mapping of phenol degradation genes from

Bacillus stearthermophilus FDTP-3 and their expression in E coli.

Applied & Enviromental Microbiology 1992, 58(8): 2531-2535.

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