Quality Control Maintenance

TERESA BRAKE

**** ********** ***** *****, *******, NC 27610

919-***-****

********@*****.***

Summary Specialized knowledge in biology utilizing DNA extraction,

real-time PCR, DNA fragment analysis and DNA sequence analysis

Demonstrated achiever with exceptional knowledge of laboratory

maintenance and sterile techniques

Ability to prioritize and perform multiple tasks in a dynamic

environment

Strong attention to detail, adheres to Quality Control

procedures, reports any failure or adverse trend in QC

Ability to prioritize hospital and jeopardy specimen

Education

B.S. Degree Biological Sciences 1995

North Carolina State University, Raleigh, NC

Career History & Accomplishments

Senior Technologist, Laboratory Corporation of 1999-2014

America, RTP Inc.

Team Leader over Hepatitis Qualitative test

Order reagents and other laboratory supplies to

stock lab, worksheet maintenance, inventory and

managing TAT, Cobas. Manage quarterly internal

inventory audits

Conducted Hepatitis C Genotyping, including

isolation of plasma, Polymerase Chain Reaction

(PCR), gel electrophoresis, cycle sequencing, isobot

automation and genotypic analysis

Use of Auto-LIPA 48, to process HCV Geno results

Roche Diagnostics real-time experience with

Ampliprep and Taqman

Operates genescan software on the ABI Prism 377 DNA

sequencer, along with ABI prism 3700 sequencer

Experience in analyzing HIV genotyping and GLP/GCP

Responsible for reviewing and verifying assays for

technologists, auditing information to ensure that

reporting is correct

Technologist II, Laboratory Corporation of America 1997-1999

RTP, N.C.

Worked with infectious diseases, HIV-1 Monitor kit,

a quantitative assay that determines the viral load

of the disease

Validated on Hepatitis C qualitative and

quantitative PCR assays

Responsible for performing and validating Cystic

Fibrosis assays for use on ABI Prism 377 DNA

Sequencer

Technologist I, Laboratory Corporation of America 1995-1997

RTP, N.C.

Prepared ladder controls for paternity and forensic

testing through PCR

Assisted with production and allocation of lab

reagents and production of Identity Multiplex Allele

Ladders

Isolated DNA from specimens for restriction enzyme

digest for a Glaxo Wellcome's clinical trial

Familiar with Qiagen and Puragen DNA prepping

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