Medical Manager

Yaojuan Lu

**** * ***** *** *******, IL *****

630-***-**** *********@*****.***

Qualifications Summary

Dedicated and work focused Research Assistant with more than 14 years molecular biology

working experience.

Exceptional capacity to multitasks: prioritize tasks while deliver excellent results;

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Solid administrative experience include lab management and assessment of workload;

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Excellent Technologist and an outstanding team worker;

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Outstanding communication skills with superior accuracy in patient records;

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Certification and Proficiencies

DNA/RNA extraction and purification;

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Conventional PCR, RT PCR, quantitative PCR;

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DNA sequencing and mutation analysis;

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Animal husbandry, transgenic mouse breeding, PCR genotyping etc.

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Experience with Molecular cloning (Subcloning) and transgenic constructs;

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Cell culture, Transfection;

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Histology (Embedding, section and staining);

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Immunohistochemistry Staining;

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Protein isolation and purification;

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Western Blotting

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ELISA;

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Chromatin Immunoprecipitation (ChIP);

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LightShift Chemiluminescent EMSA;

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Career Experience

Rush University Medical Center, Chicago, Illinois

Research Assistant, 2007 Present: Served as Technologist and lab manager. Order lab supply,

coordinate with clinical staff for sample receiving and processing; Carry out molecular

experiments; responsible for arrange lab equipment usage and repairs. Contribute to experiment

plan design and grant applications; Help training lab new recruits and student rotations; keep good

records and help maintain lab database. Excellent computer skills. The major projects are

transgenic mouse and conditional knowout mouse model, from constructs to mouse husbandry,

genotyping, and breeding.

Baylor College of Medicine, Houston, Texas

Research Associate, 2000 2007; Basic and clinical research tasks related to Pediatric oncology,

including DNA/RNA/Protein related works, processing clinical samples, keep meticulous records

of all samples. Follow all the regulations about clinical sample processing. Excellent work was

recognized as demonstrated by promotion three times during working in Texas Children’s Hospital,

Baylor College of Medicine.

Education and Training

ASCP certified Molecular Biology Technologist (MB)

M.S. China Institute of Radiation Protection, Taiyan, China

M. D. Norman Bethune Medical School (Jilin University), Chanchun, China;

Reference

1. Qiping Zheng: ************@****.***, 312-***-**** (Rush Medical Center)

2. Carl Maki: *********@****.***, 312-***-**** (Rush Medical Center)

3. Ching C Lau: *****@****.***, 832-***-**** (Baylor College of Medicine)

Publications

1 Lu Y, Qiao L, Gu J, and Zheng Q (2014). Col10a1 gene expression and chondrocyte

hypertrophy during skeletal development and disease. Frontiers in Biology 2014: (In Press,

invited review).

2 Lu Y, Gitelis S, Ding M, Maki C, and Zheng Q (2014). Research findings working with the

p53 and Rb1 targeted osteosarcoma mouse model. Am J Cancer Res 2014: (In Press).

3 Gu J, Lu, Y., Qiao, L., Ran, D., Li, N., Cao, H, Gao Y, and Zheng, Q. (2013). Mouse p63

variants and chondrogenesis. Int J Clin Exp Pathol. 2013 Nov 15;6(12):2872-9. eCollection

2013.

4 Gu J, Liang, Y., Qiao, L., Li, X., Li, X., Lu, Y., and Zheng, Q. (2013). Expression analysis of

URI/RMP gene expression in cervical cancer by high-throughput tissue microarray analysis . Int J

Clin Exp Pathol. 2013 Oct 15;6(11):2396-403. eCollection 2013.

5 Gu J, Li, X., Liang, Y., Qiao, L., Ran, D., Lu, Y., Li, X., Wei, W, and Zheng, Q. (2013).

Upregulation of URI/RMP gene expression in cervical cancer by high-throughput tissue

microarray analysis. Int J Clin Exp Pathol. 2013;6(4):669-77. Epub 2013 Mar 15.

6 Lu Y, Abassi S, Li F, Ding, M, Wu G, Gu J, and Zheng, Q. (2013). Distinct function of P63

isoforms during embryonic skeletal development. Gene Feb 26; 519(2):251-259 (2013), doi:

10.1016/j.gene.2013.02.021. [Epub ahead of print].

7 Ding, M; Abbassi, S; Li, J; Lu, Y; Geoffroy V; Li, F; Plaas, A., and Zheng, Q. (2010). Runx2

regulation of Col10a1 expression during chondrocyte maturation impacts bone formation and

joint maintenance Rush Orthopedic Journal. (1):56-57 2010.

8 Li, F; Lu, Y; Abbassi, S; Ding, M; Chen, Y; Wang, S; Lee, B; and Zheng, Q. (2010).

Characterization of the hypertrophic chondrocyte-specific Col10a1 cis-enhancer both in vitro

and in vivo. Rush Orthopedic Journal. (1):64-65 2010.

9 Li, F., Lu, Y., Ding, M., Napierala, D., Abbassi, S., Chen, Y., Duan, X., Wang, S., Lee, B.,

and Zheng., Q (2011): Runx2 contributes to murine Col10a1 gene regulation through direct

interaction with its cis-enhancer. J Bone Miner Res. 26(12):2899-2910

10 Li, F., Lu, Y., Ding, M., Wu, G, Sinha, S., Wang, S., and Zheng, Q (2012). Putative function

of TAP63α during endochondral bone formation. Gene Mar 10; 495(2):95-103 (2012),

doi:10.1016/j.gene.2011.12.057

11 Ding, M., Lu, Y., Abbassi, S., Sumner, D., Li, F., Song, Y., Geoffroy, V., and Zheng, Q.,

(2012): Targeting Runx2 expression in hypertrophic chondrocytes impairs endochondral

ossification during early skeletal development. J Cell Physiol. 2012 Oct;227(10):3446-56. doi:

10.1002/jcp.24045.

12 Lu Y, Sun Shiqun: X-ray micro-region spectrum energy analysis of Yunnan tin miner’s lung

cancer. Chinese Journal of Pathology 21(2): 103-104(1992)

13 Ljungberg MC, Bhattacharjee MB, Lu Y, Armstrong DL, Yoshor D, Swann JW, Sheldon M,

D'Arcangelo G: Activation of mammalian target of rapamycin in cytomegalic neurons of human

cortical dysplasia. Ann Neurol. 2006 Oct;60(4):420-9.

14 Lu XY, Lu Y, Zhao YJ, Jaeweon K, Kang J, Xiao-Nan L, Ge G, Meyer R, Perlaky L, Hicks

J, Chintagumpala M, Cai WW, Ladanyi M, Gorlick R, Lau CC, Pati D, Sheldon M, Rao PH.:

Cell cycle regulator gene CDC5L, a potential target for 6p12-p21 amplicon in osteosarcoma.

Mol Cancer Res. 2008 Jun;6(6):937-46.

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