Pvt Ltd Project

CURRICULUM VITAE

Personal Information:

Name: Mr.Amol Shivaji Chaugule

Address: A/P Prayag Chikhali, Karveer Tal., Kolhapur District, Maharashtra.

Phone number: +91-805*******, +918*********

18th Oct.1988

Date of Birth:

*************@*****.***, ******@*****.***

E-mail:

Career Objective:

To establish myself as one of the best cadres your organization has recruited, through ethical

application of my skills and strive for the growth of the organization.

Research Experience: (2 year& 10 month)

Designation Name of Employer Year of Working

Research Associate Taiyo Lucid Pvt Ltd. Aurangabad Nov.2013 – Till date

Research Associate Codon Bioscience Pvt Ltd. Goa Sep.2012 – Oct.2013

Project Associate Hi-Tech Biosciences India Ltd, Pune June.2011 – Sep.2012

Projects Undertaken:

2013- Cont. Working on product development for Dietary fiber, nutritional supplements and

plant extract for Diabetics, Cardiac and Bone health.

2012 – 2013 Worked on cellulase and xylanase production from actinomycetes

2012 – 2013 Worked on enzymatic clarification of fruit juice by using pectinase and amylase.

2012 – 2013 Worked on novel actinomycetes isolation for lignocellulose degrading enzymes

2011 – 2012 Worked on downstream processing of Lipase enzyme

2010 – 2011 Worked on a project entitled “Decolorization of textile dye Yellow HDF by

Pseudomonas desmolyticum

2008 – 2009 Worked on annual project on production of Alginate from Azotobactersps.

Academic Qualification:

Degree University/Institute Year Marks M.Sc. Biochemistry Shivaji University, Kolhapur 2011 65.70

B.Sc. Biotechnology Shivaji University, Kolhapur 2009 64.38

Intermediate Maharashtra State Board 2006 60.00

10thClass Maharashtra State Board 2004 69.06

Research and Technical Skills:

Work to maintain laboratory operations

Experience in managing multiple projects

Evaluating and developing new protocols

Working independently as well as in teams in competitive environment

Provide technical expertise by trouble shooting analytical sample analysis issues

Research work Analysis, Report Generation and Data creation

Training and Development

Excellent analytical and organizational skills

Good oral and communication skills

RESEARCH EXPERTISE

Downstream processing of- Lipase and Nitrilase

Lignocellulolytic degradation process- Cellulase, Xylanase, and

beta-glucosidase

Saccharification process –Pectinase, Isoamylase and Pullulanase

Herbal extract preparation– Salacia, Moringa and Tulsi.

Biochemistry techniques

Chromatography

High pressure liquid chromatography

Molecular weight determination and purification by size exclusion chromatography

Ion exchange chromatography

Hydrophobic interaction chromatography

Quantative and Characterization techniques

Glucose estimation by GOPOD and Vako test kit.

Enzyme assay by Spectrophotometric method

Fat analysis by Soxhslet

Protein Stability assays (Thermal and pH)

Colorimetric methods

Protein quantitation by Gerhatdt Vapodest, Bradford, Lowry and UV methods

Enzyme Kinetics and Inhibitor studies

Km, Vmax and Kcat determination

Identification of Enzyme activators and Inhibitors

Electrophoresis methods

SDS-PAGE

Native PAGE gel for Zymogram analysis

Protein refolding methods

Dialysis and Dilution methods

Enzyme and product concentration methods

Spray Dryer

Stirred Cell Ultrafiltration

Tangential Flow Filtration

Precipitation methods

Biological sample Lysis

Enzymatic cell Lysis

Mechanical cell disruptor (Sonicator and Homogenizer)

Microbiology techniques

Isolation of pure bacterial and fungal colonies from contaminated soil and water

Media optimization and culture condition optimization

Basic techniques like streaking, spreading and Microscopy.

Bacterial cells and fungal spores long term freezing techniques

Computer skills

Graphics software: Microsoft Office (MS Word, PowerPoint, Excel)

REFERENCES:

Dr. Amar Telke,

Group Leader, R&D

Codon Biosciences Pvt Ltd, Goa.

Email: *******@*****.***

Mobile: +91-963*******

Dr. Chetan Joshi,

Group Leader, R&D

Hi-Tech Biosciences India Ltd, Pune

Email:**********@*****.***

Mobile: +91-988*******

DECLARATION:

I hereby declare that all the information and statements mentioned above are true, complete and

correct to the best of my knowledge and belief.

(Amol Chaugule)

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