Assistant Technician

* ******* **** ******

N ATALIA AMOS HUERTA

PO BOX 60561 NY 112**-***-*** 6097 ********@*****.***

O BJECTIVE

Seeking a position where my experience as a graduated biochemist and assisting in

research could be effective, as well as acquiring added skills, in the avocation of the

desired outcome. Interested in a wide scope of biological science in any capacity.

Specific pursuit of study is infectious diseases and differential diagnostics. Highly

dexterous at research in various libraries. Analytically focused in execution of problem-

solving.

S PECIFIC EXPERIENCE & C OMP ETENCIES

P CR A GAR FORMING

R NA/ DNA SPLITTING/S TAINING N MR/IR SPECTROSCOPY

C ERTIFIED COMPUTER P ROGRAMER

E DUCATION

New York University 2013-Present

M.S. Molecular Biology

Pending Graduation:

Roosevelt University 2008-2010

B.S. BIOCHEMISTY

HONORS PHI THETA CAPPA

P ROFESSIONAL HISTORY

Companionate Green Financial 2010-2011

Consultant

Employed as part of a team of specialist who worked with clients that were interested in devising way to make

products that would use the least amount of chemicals. Developed and devised proposal for custom projects that

varied in demand. Maintained reports and man aged with laboratory equipment suppliers. Point person for any

and all science-related inquires and strategies.

Additional highlights:

Researched & devised methods for customized proposition

Wrote manuals of methods competency

Harold Washington College 2009-2010

Assistant to Head of Laboratory Technician and Professor of General Chemistry

Maintained materials and receipts for chemical equipment and organized invoices. Prepared chemical

experiments, to be tested and confirmed by supervisors. Managed inventory and created reports of needed items.

Maintained a clean laboratory environment. Prepared stained slide of biological material for professors and aided

H UERTA 2

in helping students when needed. Assisted in student inquires of experiment assigned. Performed additional lab

duties such as cleaning, calibrating, equipment maintenance, creating and prepping reagents and standards,

reviewing and editing logbook entries, stocking and reordering chemicals and supplies, keeping SOPs current,

sorting/organizing/updating recorded data, properly reporting and rerunning failed samples.

Additional highlights:

Production of agar plating

Treating and preparation of chemicals solvents and making dilutions

NMR plating and data graphing via software

DNA splitting

Preparation of various bacteria for viewing

AREAS OF SPEACIALIZATION:

Organic & General Chemistry:

Measured melting points, paper chromatography, micro and macro -scale solvent extraction,

recrystallization/sublimation, micro-scale reflux apparatus, infrared spectroscopy. Prepared and identified esters,

synthesizing acetylsalicylic acid, saponification of fat using vacuum filtration, steam distillation and thin -layer

chromatography solution preparation, titrations, extractions filtrations, separations, simple and fractional

distillation, melting point and (micro) boiling point determination, solubility tests, determination of index of

refraction, freezing point depression, simple syntheses, thin-layer chromatography, liquid chromatography, gas

chromatography, reflux recrystallization

Biochemistry:

Used spectrophotometer, gel filtration chromatography, extraction and assaying to determine substrate and enzyme

concentration, extraction and separation of polar and non -polar lipids. DNA extraction, plasmid DNA preparation,

restriction enzyme digests, PCR, agarose gel electrophoresis, cell fractionation by differential centrifugation

Microbiology:

Aseptic and sterile techniques, optical microscopy, bacterial staining, plating methods (streak, spread, pour,

replica), enumeration and identification of bacteria, use of biological safety cabinets, media and buffer prepara tion,

water sample analysis by membrane filtration and MPN, diagnostic biochemical tests for enteric bacteria, use of

selective media, Staph latex slide agglutination test, serotyping, antibiotic assay (MIC), plaque assay, conjugation

Genetics:

Measured DNA extraction and O/D via spectrophotometer, B -Galactosidase synthesis in E.coli culture,

micropipette and electrophoresis of hemoglobin variants, recombination and cross -over using tetrad analysis,

transformation of E.coli via F-plasmids, PCR and DNA fingerprinting using clinical centrifuge, microfuge,

automated thermocycler, agarose gel electrophoresis and UV transilluminator, restriction endonuclease digestion

of DNA.

Computer Skills:

Programing (JAVA, C++, C#), extensive use of Word, Excel, Access and PowerPoint for Macintosh and PC

platforms, typing (50 words/minute), PubMed, Minitab

LANGUAGES

English – native language

Spanish – speak fluently and read/write with high proficiency

Hebrew- Moderate in reading/writing and speaking

Contact this candidate