Don Ruwan Ranatunga
**** ******* ***, ********* **, Madison, WI 53705. Phone: 920-***-****
******@*****.***
Education Masters Certificate of Project Management 2012
University of Wisconsin Madison, Madison WI
Master of Science in Biology/Microbiology 2009
University of Wisconsin Oshkosh, Oshkosh WI
Bachelor of Science in Microbiology 2004
University of Wisconsin Oshkosh, Oshkosh WI
Professional Experience
Research Specialist, Great Lakes Bioenergy Research Center, Madison, Wisconsin – 2009 to date
Took leadership in process development and SOP preparation to increase efficiency of the
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fermentation facility
Designed experiments to solve specific problems related to ethanol production using bacteria
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and yeasts
Helped maintaining a state of the art fermentation facility with bioreactors ranging from 10 mL
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to 12 L
Coordinated and collaborated experiments with different research groups within GLBRC
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Analyzed data generated by instruments such as off-gas analyzers, bioreactors,
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spectrophotometers, HPLC-RID.
Trained and supervised research assistants
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Gained experience in using software platforms designed to operate equipment by DasGip,
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Sartorius, Applikon and TECAN
Gained experience setting up chemostat experiments
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Coordinated management of GLBRC feedstock biomass analyses and communicated results
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Coordinated, managed and supported GLBRC hydrolysate analytical pipeline and
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communicated results
Coordinated with feedstock and hydrolysate stakeholders to ensure needs and concerns were
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addressed
Participated in preparing a written report to Administrative Team (done quarterly or as needed)
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on feedstock and hydrolysate analytical pipeline issues and/or progress
Shipped feedstock biomass to requestors and provided oversight of biomass inventories
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Worked with the GLBRC Metabolomics facility to provide support services and data analysis
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Collaborated and coordinated with GLBRC research project leaders to implement assays that
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address hydrolysate research questions, under the direction of the GLBRC Enabling
Technologies (ET) Director
Collaborated and coordinated with GLBRC research project leaders to implement
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fermentations through utilization of ET equipment or other available Center units, under the
direction of the GLBRC Enabling Technologies Director
Don Ruwan Ranatunga
2
Research Student in Protein Chemistry, UW Oshkosh, Oshkosh, Wisconsin – 2006 – 2009
Cloned genes and expressed in E.coli plasmids
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Grew bacterial cells in fermenter cultures
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Produced proteins and purified those using FPLC and gel electrophoresis
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Studied the structure and function of Cytochrome bf and Plastocyanin in cyanobacteria
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Computer and Instrumental Skills
Microsoft Word, Excel, Power Point, Internet, e-mail, Laboratory Information Management
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Software – STARLIMS, Genetic modeling software – Gene Construction Kit (GCK),
Laboratory automation software - LabView.
Fermentors by DasGip, Applikon and Sartorius Stedim, High-throughput plate readers by
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TECAN, NOVA BP300 bioanalyzer, MGA iScan off-gas analyzer, Spectrophotometers,
Fluorescence microscopy, GC, LC, UPLC and FPLC machines (Pharmacia), PCR techniques..
Additional Experience
Computer Technician – Management Information Office (M.I.O) of UW Oshkosh – 2006 to 2008
Troubleshot computer related problems of Residence Life computers and residents’ computers.
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Setup workstations for conferences.
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Residence Hall Information Specialist (R.H.I.S.) - M.I.O. of UW Oshkosh - 2001 to 2004
Managed a residence hall computer lab and assisted residents in computer related technical
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problems and software problems.
Provided feedback on possible improvements regarding the computer lab to M.I.O and to the
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Residence Hall Director.
Publications
Youngquist, J. T., Lennen, R. M., Ranatunga, D. R., Bothfeld, W. H., II, W. D. M. and Pfleger,
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B. F. (2012), Kinetic Modeling of Free Fatty Acid Production in Escherichia coli Based on
Continuous Cultivation of a Plasmid Free Strain. Biotechnology and Bioengineering.
doi: 10.1002/bit.24420
Rebecca M. Lennen, Max A. Kruziki, Kritika Kumar, Robert A. Zinkel, Kristin E. Burnum,
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Mary S. Lipton, Spencer W. Hoover, Don R. Ranatunga, Tyler M. Wittkopp, Wesley D.
Marner II, Brian F. Pfleger. (2011), Membrane Stresses Induced by Overproduction of Free
Fatty Acids in Escherichia coli. Applied and Environmental Microbiology, 77(22):8114. DOI:
10.1128/AEM.05421-11.
“Expression and Purification of the Chlamydomonas Blue-Copper Plastocyanin Protein,”
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Masters’ Thesis, Department of Letters and Science, University of Wisconsin Oshkosh – 2009
References:
Steven C. Slater, Ph.D. Yaoping Zhang, Ph.D. Victor Alatorre, MBA
Scientific Programs Manager Senior Scientist Assistant Director of Residence
Great Lakes Bioenergy Research GLBRC Life
Center (GLBRC) University of Wisconsin
4154 WEI
5129 Wisconsin Energy Institute Oshkosh
5129 Wisconsin Energy Institute
1552 University Ave. 800 Algoma Blvd.
1552 University Ave.
Madison, WI53726 Oshkosh, WI 54901
Madison, WI53726
608-***-**** ********@*****.***
******@*****.****.***
********@*****.****.***