Biochemist/Molecular Biologist
Resume:
PAYAL KHANNA
*****************@*****.***
http://www.linkedin.com/pub/payal-khanna/30/86a/626
OBJECTIVE: To obtain a position as a Biochemist/Molecular Biologist
EDUCATION
Albany College of Pharmacy and Health Sciences, Albany, NY September 2012- May 2014(expected)
Candidate for MS in Pharmaceutical Sciences (Pharmacology track), GPA 3.7
St. John’s University, NY September 2011-August 2012
Completed a year of graduate studies in Pharmacology
Dr. Bhanuben Nanavati College of Pharmacy, India July 2007- May 2011
University of Mumbai, BS in Pharmaceutical Sciences
RELATED EXPERIENCE
Graduate Research Assistant (Molecular biology lab), January 2013- present
Albany College of Pharmacy and Health Sciences, NY
• Performed nucleic acid isolation, purification and quantification
• Assisted my PI in confocal microscopy and protein quantification assays
• Performed cell transfection, gene knockdown techniques and plasmid engineering
• Assisted my PI in his experiments and troubleshooting and designing protocols
• Also helped in maintaining lab supplies inventory and equipment, handling process orders and helping the
undergraduate students which improved my interpersonal and verbal skills
• Documented experimental results, thus developed proficiency with MS Office(Word, Excel, Power Point) and
Adobe Photoshop
• Assisted in writing scientific articles
• Presented experimental results and data at lab meetings which enhanced communication skills
• Proficient in data analysis
St. John’s University, NY May 2012- August 2012
Conducted preliminary studies for Research Project in a Biochemistry lab at St. John’s University which included:
• Mammalian cell culturing sterile techniques, maintenance and characterization of multiple cell lines
• Cell based Cytotoxicity Assays (MTT) on multiple cell lines including Jukart cells,SKMEL-2 cells, HELA cells,
HEK293 cells using Cisplatin and Pateamine analogs
• Handled Jukart cells, MDAMB2 cells, SKMEL-2 cells, HELA cells, HEK293 cells, performed passaging and
splitting of these cell lines
• Preparation and transformation of BL-21 and DH5α competent cells
• Performed RNA isolation using RNase easy Mini kit
• Transformation of HCV cap dependent plasmid into Escherichia Coli (strain DH-5)
PAYAL KHANNA
As an Undergraduate Pharmaceutical Lab Student at University of Mumbai, received training in: July 2007-
May 2011
• Basic Microbiology Techniques- working in aseptic area, handling microorganisms, bacterial cells
• Operated UV Visible Spectrophotometer, photo colorimeter and pH Meter
• Familiar with lab techniques such as Histology, Staining of tissues, titration and Volumetric Assays
RESEARCH PROJECTS
1. MS thesis -“VDUP1-dependent regulation of neuroblast proliferation and progeny differentiation in the Drosophila
CNS”
• Set up various crosses and mutants of the fruit fly Drosophila Melanogaster using gene knockdown and gene
expression techniques
• Dissecting the adult and larval fly brains and performing immunohistochemistry
• Mounting the brains and analyzing using confocal microscope and immunofluorescence
• Quantifying the gene expression or knockdown using western blots and RT-PCR
• Quantifying the expression of proteins by measuring their fluorescence intensity using CellSens Dimension
2. Molecular Cloning-Designed a new plasmid vector construct pUAST VDUP1-GFP for assessing the upregulation
of VDUP1
• Gel purified the plasmid vector using ultra clean gel spin DNA purification kit
• Cut the vector using restriction enzymes and then performed DNA ligation
• Transformation/ transfection of competent Escherichia Coli cells
• Isolated plasmid DNA using plasmid mini prep kit
• Analysis of VDUP1-GFP miniprep DNA
• Running polymerase chain reaction (PCR) to verify the VDUP1-GFP insert
• Analyzed the insert using gel electrophoresis
• The DNA was quantified and sent to Rainbow transgenics for genetic engineering of the vector into the fruit fly
Drosophila embryos thus developing a new mutant fly for further analysis for tumorigenesis
WORK EXPERIENCE
Indoco Remedies, Mumbai May-June 2010
Intern
PAYAL KHANNA
• Formulation and Development Department in Tablet, capsule, oral liquids manufacturing and troubleshooting
• Quality Control and Quality Assurance laboratory
• Design and Manufacture of equipment used in the formulation department
• Analytical department- analyzing samples using HPLC, GC and troubleshooting
POSTER PRESENTATIONS
• “The VDUP1 tumor suppressor gene negatively regulates larval Drosophila central brain growth by controlling
neuroblast proliferation and progeny differentiation.” at the “Neurobiology of Drosophila” conference held in
October 2013 at Cold Spring Harbor Laboratory, NY
• “Sterilization & its novel applications” at the Rx Festival organized by the Indian Pharmaceutical Association(IPA)
(2009)
• “Antidepressants-role of nicotine as an antidepressant” at the UICT- Exergy Tech Fest (2010)
• “Recombinant DNA technology-its future” at NMIMS-Isthmus (2010)
• “Oleoyl Serine- A potentially viable template for treating osteoporosis” at UICT- Exergy Tech Fest (2011)
• “Novel non-invasive drug delivery system for pulmonary tuberculosis” at the Rx Fest (2011)
• “Nanosuspensions- The next Blockbuster” at UICT- Exergy Tech Fest (2011)
ACHIEVEMENTS
Winner at Institute Of Chemical Technology Fest-“Exergy 2011", Mumbai University for paper presentation on “Oleoyl
Serine a Potentially viable drug template for treating Osteoporosis.”
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