Research Assistant Co-op

AVINASH CHINTHALA

** ***** ******, ***. *, Boston, MA 02120 ? *********.*@*****.***.***?

816-***-****

Professional Summary

Actively seeking an entry-level full time/contract position in the

Biopharmaceutical industry. Extensive experience in mammalian cell culture

and transfection, protein expression, receptor isolation, purification and

analysis. Additional experience with gel electrophoresis, western blot,

ELISA, PCR, Mass Spectrometry analysis and molecular biology techniques.

Efficient, detail oriented researcher with excellent multi-tasking skills.

Being a fast learner is my major strong point. Ability to work

independently and in multidisciplinary teams. Aims to work in a challenging

and a team-oriented R&D environment leveraging technical expertise,

organization skills and adaptability.

education

Northeastern University, Boston, MA

Masters in Biotechnology (Molecular Track)

January 2014

(CGPA - 3.4)

Jawaharlal Nehru Technological University, Hyderabad, India

Bachelor of Pharmacy (CGPA - 3.83) June 2010

Biotechnology Competencies

Biochemical Techniques: ELISA, SDS PAGE, Western blot, Dot blot,

Bradford, BCA, Size-exclusion

and nickel affinity chromatography,

FPLC (Akta), UV-Visible

Spectroscopy, membrane

protein purification.

Cell Biology Techniques: Mammalian cell culture, transient transfection,

Sterile techniques.

Analytical Techniques: TLC, UPLC, SEC, IEF, ToF LC-MS, Glycoprotein

estimation,

1D/2D Gel Electrophoresis.

Molecular techniques: Transfection, Transformation, PCR, qPCR,

Isolation, Restriction

Digestion and Ligation of

DNA and quality analysis.

Bioinformatics Tools: BLAST, RASMOL, Jmol/Pymol, SPSS, NCBI, Entrez

and Protein Data

Bank and MS Office Suite.

Work Experience

DiscX, LLC., Waltham, MA

8/13 - 12/13

Research Assistant Co-op

. Conducted method development experiments exploring multiple reaction

chemistries for membrane protein nanoparticle formation.

. Cultured and transfected HEK293 cells. Successfully expressed and

purified the class-B GPCR Glucagon receptor (GCGR) using FLAG affinity

chromatography.

. Purified milligram quantities of nanodisc scaffold protein from E.Coli

cultures using Ni-affinity chromatography. Incorporated GCGR membrane

protein into lipid Nanodisc.

. Successfully developed nanodisc cross-linking methods, evaluated

various cross-linking reagents. Improved the efficiency, stability

and effectiveness of Nanodisc crosslinking.

. Analyzed the GCGR proteins and nanodiscs using LC-MS, HPLC, Size

exclusion, SDS-PAGE and Western Blot techniques.

. Documented experimental results, wrote reports.

. Interacting with supervisor, actively analyzed objectives and

accomplishments.

. Scientific presentations in group meetings.

. Worked in compliance with company EHS guidelines.

Ranbaxy Laboratories Ltd., Hyderabad, India

Research Assistant

9/09-4/10

. Collaborated on a BCA assay design.

. Physical characterization and testing for pharmaceutical APIs.

. Performed cytotoxicity assay, ELISA, and Western blot.

. Transfection and cell passaging, biochemical characterization, test

antimicrobial activity.

Research Experience

Northeastern University, College of Science

9/12 - 4/13

. Performed purification and characterization of antibodies. Performed

protein aggregate analysis, variant analysis, quantitation,

purification and Immunoassay using modern proteomic techniques

including peptide mapping, glycan analysis and LC-MS/MS of proteins

produced. Chromatography including SEC, IEX and RP.

. Designed and optimized an enhanced production module, verified

statistical significance of 'drug product' production improvement (t-

test), tested productivity in a scaled-up unit.

. Investigated strategies for increasing the productivity of desired

pharmaceutical compounds from cultures, by altering the experimental

design, medium composition and testing different inducers.

. Worked with Agilent 1100 and Agilent 1220 with Chemstation software.

. Completed Masters level courses in Analytical Separation &

Characterization, Principles of Mass Spectrometry and Protein

Biochemistry and Molecular Biology.

JNT University, College of Pharmacy

8/08 - 8/09

. Preparation and Quantitative analysis of organic compounds.

. Estimation of metabolites in blood and urine.

. Sterility testing of pharmaceutical products.

. Preparation, Evaluation and packaging of various types of Drug

Formulations.

. Study and characterize the effects of CNS Stimulants on isolated

animal tissue samples, record CRC curves.

REFERENCES AVAILABLE UPON REQUEST

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