Testing Medical

Brandon Hahnlen

**********@*****.***

*** ***** ***** **. ***. 207 Daniel Island, SC 29492 419-***-****

Career Objective:

To obtain a Research and Development position by applying my educational background in Molecular Biology, Chemistry,

and Biochemistry while drawing on my past experiences in various research fields.

Education:

Kent State University, Kent, OH

May 2010

Bachelor of Science in Biology, Molecular and Cellular Biology with a Minor in Chemistry

• Major G.P.A. 3.3

• Reviewed Academic Primary Literatures covering recent research topics in molecular biology,

neuroscience, immunology, and cancer as part of the writing intensive requirement of the Kent State Biology

Department

• Science courses taken include:

Cellular Biology Microbiology Neuroscience Inorganic Chemistry

Molecular Biology Immunology Biochemistry I and II Physics I and II

Cancer Biology Human Genetics Organic Chemistry I and II Organic Evolution

Related Work Experience:

Medical University of South Carolina

Department of Drug Discovery and Biomedical Sciences

September 2013 – Present

Research Specialist II

• Maintained multiple mammalian cell lines as well as a wild type/ GST pi knockout mouse colony

• Primary specialist assigned to “sulfiredoxin” project

• Performed numerous molecular biological techniques including:

PCR Western Blots Transfections DNA extraction

SDS-PAGE Protein Purifications Immunoprecipatations DNA purification

Genotyping Bacterial Culturing Flow Cytometry Genomic Mutations

• Calculated, prepared, and adjusted the molarity and pH of multiple solutions needed for a diversity of

testing assays

• Use of aseptic technique in Biohazard Level 2 environment

• Experience in handling and testing strains of E. coli and the use of lentiviruses

• Development of experimental design in collaboration with post-doctoral scholars and professors by

organizing data, preparing presentations, participating in weekly journal clubs to discuss ongoing research within

the lab, and analyzing and interpreting results of experimentation

• Order supplies and maintain stock of laboratory equipment

North American Science Associates, Northwood, OH

July 2012- July 2013

InVitro Technologist II

• Performed multiple cytotoxicity testing methods including: Elution Method, Agarose Overlay, and Direct

Contact

• Primary technologist for all hemolysis testing (extract and direct contact methods)

• Primary technologist for all C3a and SC5b-9 Complement Activation Assays (ELISA)

• Performed Partial Thromboplastin Time Assay

• Maintained multiple mammalian cell lines for testing

• Analyzed results using Prism Statistical software

• Performed all testing under direction of NAMSA SOPs, study director derived protocols

• Familiar with regulations: ISO 10993 Part 4, Part 5, and Part 12, ASTM F756, ASTM 2382, ASTM 2567,

MHLW standards

• Collaborated with laboratory management, medical research managers, clients, and toxicologists in

development of Mechanical Hemolysis Protocols

August 2010 – June 2012

Toxicology Lab Technician III

• Performed biocompatibility testing, specifically toxicity testing in mice including intraperitoneal/intravenous

dosing, clinical observations, and blood collection

• Collected data in compliance with the NAMSA standard operating procedure for data collection

• Assisted surgical staff during study terminations

• Collaborated with the toxicology team, the supervisor for the standard testing group, and clients for

special, non-routine studies

• Experienced in the use of Laboratory Information Management System (LIMS)

• Worked in a Good Laboratory Practice and Good Manufacturing Practice (GLP/GMP) environment,

following direction of study director derived protocols and NAMSA standard operating procedures

• Familiar and compliant with International Organization for Standards (ISO), United States Pharmacopeia

National Formulary (USP-NF), and Institutional Animal Care and Use Committee (IACUC) regulations

Field Experiences:

Toledo Hospital Histology Laboratory, Toledo, OH

Summer 2009 – Fall 2009

Observation

• Observed gross dissections of numerous and diverse specimens

• Gained knowledge in staining techniques and dissection methods

• Under direction from Dr. Gene Richard observed analysis of slides including cancerous cells through light

microscopy

Toledo Hospital Anesthesiology Department, Toledo, OH

Summer 2009 – Fall 2009

Observation

• Observed surgeries including open heart, lobectomy, and transsphenoidal

• Gained knowledge in anesthesiology techniques

Awards and Honors:

• Access Kent Scholarship Recipient (Fall 2006 – Spring 2010)

• Dean’s List (Spring 2008, Fall 2008)

Additional Skills and Qualifications:

• Pre-Medicine Club Member (2006 – 2010)

• Proficient in Microsoft Word, Excel, and PowerPoint

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