Production Scientist

Amanda Lyons

*** *********** *****, ******** *******, GA 30002

404-***-**** accpqu@r.postjobfree.com

EDUCATION:

Georgia State University Atlanta, GA 05/2008

Master of Science, Biology

Biotechnology concentration

Presented research poster at SFN Neuroscience Conference, San Diego, CA 11/2007

GPA 4.0

Georgia State University Atlanta, GA 05/2007

Bachelor of Science, Biology

HOPE scholarship

Biotechnology scholar

Cumulative GPA 3.2, Major GPA 3.8

LABORATORY SKILLS:

Aseptic Technique Fluorescence Microscopy

Protein Concentration Assay Confocal Microscopy

WESTERN Blots SDS-PAGE

ELISA HPLC, FPLC

EXPERIENCE:

Centers for Disease Control and Prevention Atlanta, GA 12/2010-present

CCID/NCEZID/DSR/SPSB/Immunochemistry and Cellular Development

Associate Service Fellow

Protein purification for the bioterrorism unit, specifically antibodies against Bacillus anthracis

Antibody development, specifically antibodies against Bacillus anthracis

Antibody Labeling/Conjugation

Phage Display

Cell Culture

Hybridoma work

Proficient use and maintenance of following equipment: AKTA Prime, FPLC, AKTA Explorer, Gene Quant,

Spectramax, ForteBio, Experion; automated gel electrophoresis, and Typhoon Imager

Writing SOPs for procedural work in the laboratory

Centers for Disease Control and Prevention Atlanta, GA 10/2007-12/2010

CCID/NCEZID/DSR/Biologics Branch/Protein Science

Production Scientist

Protein purification and characterization

Antibody development

Antibody Labeling/Conjugation

Proficient use and maintenance of following equipment: AKTA Prime, FPLC, AKTA Explorer, Gene Quant,

Spectramax, Experion; automated gel electrophoresis, and Typhoon Imager

Writing SOPs for procedural work in the laboratory

Helped mitigate the H1N1 Influenza A Pandemic by supporting the Emergency Operations Center-Fall 2009

Completed Emory University’s Biosafety Level-3 Biosafety Course 08/2009

Attended PEGS (Protein Engineering Summit), Boston, MA 04/2009

Georgia State University Atlanta, GA 05/2006-05/2008

Biology Department

Research Assistant

Set up mutant constructs of Caenorhabditis elegans

Analyzed gfp mutants under light, and fluorescence microscopes

Obtained varicosity data from confocal microscope images and LSM software

Created movies of C. elegans using fluorescence dissecting microscopes and CCD-cameras

Analyzed C. elegans neuronal data using MATLAB programs

COMPUTER SKILLS:

Skilled in MS programs Word, Excel, PowerPoint, and Outlook. Experience with MATLAB software.

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