Design Development

Daniel McCoy

******.*****.*****@*****.***

Relocating to North Carolina 617-***-****

PROFESSIONAL SUMMARY

Molecular Biologist with extensive experience in both industry and academic

settings. Highly motivated and self-sufficient scientist who has a proven

track record of consistently producing reliable and insightful data.

Action-oriented with excellent communication and problem solving skills.

Demonstrated ability to meet timelines and multitask in an efficient

manner. Thrives in team environments and experienced in a variety of

methods including: cloning, DNA/RNA/Protein isolation and quantification,

PCR/qPCR assay design, mammalian cell culture, viral plaque assay, live

cell imaging, in vivo mouse models and transgenic line generation, tissue

isolation and confocal IHC/IF imaging, and ELISA. Eager to continue a

career in industry research and/or development with therapeutic

significance.

WORK EXPERIENCE

University of Southern California, Los Angeles, CA

Ph.D., Molecular Biology, August 2008-December 2013

"The expected and unexpected roles of TRPM8: Cold Pain and Metabolism"

Project: 1) TRPM8 pore dilation can deliver therapeutics to cold-pain

sensing neurons

2) Enhanced insulin clearance in mice lacking functional

TRPM8 channels

. Utilized extensive molecular cloning, live cell imaging, and mouse in

vivo techniques to independently develop 6 unique transgenic mouse

models for studying the roles of Transient Receptor Potential (TRP)

ion channels in pain

. Characterized a channel pore dilation model demonstrating that

cationic therapeutics can be delivered to cold-pain sensory neurons in

a highly specific manner. Results particularly novel and impactful as

it was previously thought to not be possible using this technique.

. Discovered and published a novel insulin clearance phenotype for the

thermosensory channel TRPM8 with therapeutic implications in regards

to the treatment of diabetes and other metabolic disorders

Acambis (Now Sanofi Pasteur), Cambridge, MA

Associate Scientist III, 2008

. Worked in the Bio-therapeutics department supporting the development

of ACAM529, a preclinical vaccine against HSV-2 (genital herpes)

. Supported both formulation and process development studies carrying

out viral plaque assays and data quantification

. Developed a chemiluminescent Southern blot protocol for identifying

modified viral HSV-2 DNA in complementing cell lines

. Carried out optimization studies for in house viral plaque assay SOP

and presented findings to the ACAM529 team

Wyeth (Now Pfizer), Cambridge, MA

Contract Scientist (2007) and Intern (2006)

. Worked in the Biomarker Discovery department handling clinical samples

in efforts to identify candidates for various inflammatory diseases

(psoriasis, asthma etc.)

. Performed ELISA analysis to support studies on inflammatory cytokine

candidates and processed clinical samples for gene chip analysis (RNA

extraction, quantification and stability studies using a Bioanalyzer)

Biogen Idec, Cambridge, MA

Intern, 2004 + 2005

. Worked as a summer intern in the Discovery Neurobiology department

(2004+2005)

. Cloned and expressed a neuroinflammatory receptor candidate in various

soluble truncated forms

. Worked with mammalian cell lines and the drosophila S3 cell line for

expression of truncation variants. Expression was analyzed by Western

blot

SKILLS/EXPERTISE

Molecular Biology

. Cloning - restriction enzyme digestion, ligation, transformation, site-

directed recombination (for BAC-transgenic mouse line targeting

constructs)

. DNA/RNA/Protein - isolation, quantification, bio-analysis,

purification, electrophoresis, SDS-PAGE,

. PCR/qPCR - cDNA library prep, primer design

. Microarray - sample prep, basic Partek Genomic Suite analysis

Imaging

. IHC/IF - tissue cryosectioning, imaging using LSM confocal and Zeiss

Apotome microscopes

. Live cell imaging - calcium imaging, dye uptake experiments with

temperature controlled liquid perfusion manipulation

. DAB labeling

. INSITU hybridization

Cell Culture

. Mammalian cell line culture - sterile technique, culturing,

transfection

. Primary sensory neuron culture - tissue dissection, cell dispersion

. Viral plaque assay - PFU titer analysis

Immunology

. Southern and Western blotting - including duplex western blotting

using a LiCoR Odyssey imager

. Immunoprecipitation

. ELISA - familiar with processing human clinical samples measuring

plasma cytokine levels as well as mouse serum samples measuring

metabolic peptides

Animal Models/methods

. Mouse Husbandry - breeding, genotyping

. In vivo models - I.P. and subcutaneous drug administration, glucose

tolerance test (GTT), insulin tolerance test (ITT), pancreatic islet

isolation, blood sampling/glucose monitoring, general isoflurane

anesthesia

. Tissue dissection - including transcardial perfusion/fixation

. Behavioral pain models - Hargreaves radiant heat assay, Von Frey,

temperature preference, acetone evaporative cooling assay, grip

strength, rotarod

Computer/Data Analysis

. Familiarity with Novell GroupWise and Microsoft Outlook

. Microsoft Office - Word, Excel - graph generation and statistical

analysis STD,SEM, TT-TEST etc., PowerPoint - extensive experience in

presenting work in PowerPoint format at regular lab meetings and

departmental talks

. Adobe Photoshop, Canvas, OriginLab, ImageJ

. Vector NTI - In-depth knowledge of tools for sequence analysis and

alignment, primer design, plasmid construction and database

organization

EDUCATION

University of Southern California, Dept. of Biological Sciences, Los

Angeles, CA

. Ph.D. in Molecular Biology, December 2013

. Dean's Young Investigator Award, USC School of Pharmacology - 2012

. Outstanding TA Award, Dept. of Biological Sciences - 2012

. Best Poster Award, MCB annual retreat - 2010

Wake Forest University, Winston-Salem, NC

. B.S. Biology, May 2007

. 7-time Dean's List

. Member - BBB Biology honors society

PUBLICATIONS

1) Lippoldt EK, Elmes RR, McCoy DD, Knowlton WM, McKemy DD. Artemin, A

Glial Cell Line-Derived Neurotrophic Factor Family Member, Induces

TRPM8- Dependent Cold Pain. Journal of Neuroscience 2013; 33(30):

12543-52.

2) McCoy DD, Zhou L, Nguyen AK, Watts AG, Donovan CM, McKemy DD.

Enhanced insulin clearance in mice lacking TRPM8 channels. American

Journal of Physiology Endocrinology & Metabolism 2013; 305(1): E78-88.

3) Knowlton WM, Palkar R, Lippoldt EK, McCoy DD, Baluch F, Chen J,

McKemy DD. A sensory-labeled line for cold: TRPM8-expressing sensory

neurons define the cellular basis for cold, cold pain, and cooling-

mediated analgesia. Journal of Neuroscience. 2013; 33(7): 2837-48.

4) Knowlton WM, Daniels RL, Palkar R, McCoy DD, McKemy DD.

Pharmacological blockade of TRPM8 ion channels alters cold and cold

pain responses in mice. PLoS One 2011; 6(9): e25894.

5) McCoy DD, Knowlton WM, McKemy DD. Scraping through the ice:

uncovering the role of TRPM8 in cold transduction. American Journal of

Physiology Regulatory Integrative and Comparative Physiology 2010;

300(6): R1278-87.

POSTERS/PRESENTATIONS

1) McCoy D, Zhou L, Nguyen A-K, Watts A, Donovan C, McKemy D. Enhanced

insulin clearance in mice lacking TRPM8 channels. Abstracts Society

for Neuroscience 2013 conference - San Diego. Poster 463.10.

2) McCoy D, McKemy D. Translational profiling approach for the molecular

characterization of TRPM8 sensory neurons. Abstracts Society for

Neuroscience 2012 conference - New Orleans. Poster 881.04.

3) Palkar R, McCoy D, McKemy D. Cell-specific permeation of large ionic

molecules into cold-sensitive neurons through TRPM8 channels.

Abstracts Society for Neuroscience 2012 conference - New Orleans.

Nanosymposium 523.

4) McCoy DD, McKemy DD. Enhanced insulin clearance in mice lacking

functional TRPM8. Abstracts AAPS Moving Targets Symposium 2012

(Metabolic Syndrome) - Los Angeles.

. Dean's Young Investigator Award for Best Poster - USC School

of Pharmacology

5) McCoy DD, McKemy DD. A Novel Role of TRPM8 in Metabolic Homeostasis.

Abstracts Annual Molecular and Computational Biology program retreat

2011 - Laguna Beach.

6) McCoy DD, McKemy DD. Translational Profiling Approach for the

Molecular Characterization of TRPM8 Sensory Neurons. Abstracts Annual

Molecular and Computational Biology program retreat 2010 - Laguna

Beach.

. Best Poster Award - USC Department of Biological Sciences

TEACHING/MENTORING

BISC 220 - General Biology: Cell Biology and Physiology

. 2012 Outstanding TA Award

. Led 2 Labs of 20 students in basic lab techniques and

experimentation

BISC 421 - Neurobiology

. Led 2 discussion sections two years (2010, 2013) reviewing lecture

material, helping students prepare for exams, and teaching/critiquing

their ability to read scientific papers and present in a cohesive

manner

Student Mentoring

. Mentored undergraduate working students in molecular biology and

imaging techniques and assisted in their making of scientific posters

to communicate findings

. Supervised two PhD graduate rotation students on their

projects, training and advising them in their experimental

paradigms

. Molecular Biology first year student mentor for new students

entering the PhD program (2009-2011)

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