Engineer Supervisor
Resume:
Yadong WEI Ph.D.
Address: Departments of Pharmacology and Molecular Sciences
Johns Hopkins University School of Medicine,
Baltimore, MD 21205, USA
Phone:
********@*****.***
E-mail:
Local resident with green card
EDUCATION:
02/2006 Ph.D. (Molecular Biology), Dong A University, Pusan, South Korea.
Thesis title: “Molecular Characterization of Beetle (Apriona germari )Cellulases”.
Supervisor: Prof. Byung Rae Jin
07/2002 M.S. (Insect molecular biology), Graduate School of Chinese Academy of Agricultural
Sciences, Beijing, China.
07/1994 B.Sc. (Sericultural molecular biology), Shandong Agricultural University, China.
ACADEMIC POSITIONS & RESEARCH EXPERIENCE:
10/2011 – present Postdoctoral Scholar
Departments of Pharmacology & Molecular Sciences, Johns Hopkins University, School of Medicine,
USA.
Supervisor: Prof. Ronald L. Schnaar
Expression of human and mouse Siglecs for bio-functional study in mammalian cells.
Expression of HIV-1 Tat proteins from clade B and clade C for study on toxicity to neuron in E. coli
and mammalian cells.
Generation of conditional knock-in transgenic mouse of human Siglec8.
Development of high affinity tools for exploring the counter-receptor of Siglec8 and Siglec9 in
human lung.
Affinity binding assay of Siglecs binding to their glycan ligands.
04/2008 –10/2011 Associate Professor
The Sericultural Research Institute. Chinese Academy of Agricultural Sciences, China
Optimization of the baculovirus expression system in the insect by using different promoters, a
novel IRES sequence, and various virus infection periods, etc. (The final goal is to improve the
expression efficiency and to express humanized glycoproteins in the insect)
Development of a new strategy to engineer silkworm N-glycosylation pathway to produce
humanized glycoproteins.
Detection of differential expressed genes in the silkworm by the comparison of genomic DNA and
cDNA microarrays.
Optimization of the Piggybac transposon system to improve the efficiency of silkworm
transgenesis.
12/2006 – 04/2008 Postdoctoral Research Associate
Institute of Human Virology, University of Maryland, Baltimore, USA.
Supervisor: Prof. Lai-xi Wang
Studies on the role of glycosylation in the vaccine to prevent HIV
Expression and purification of glycoproteins in yeast, insect baculovirus system, mammalian cells
Analyses of glycans released from glycoproteins by EndoM, EndoH, and PNGaseF using MS,
Malti-TOF, and high performance anion-exchange chromatography (HPAEC).
Syntheses of homogeneous human-like glycoproteins by the combination of yeast expression
system and in vitro chemoenzymatic transglycosylation.
In vitro transglycosylation of synthesized glycans to the specific sites of glycoproteins, such as
RNase B and monoclonal antibodies.
CURRENT REVIEWER FOR:
Journal of Asia-Pacific Entomology
PATENT:
Feed additive supplement for Nosema Bombycis inhibition. Patent Number(s): CN01127276.7
(2001-09-29).
Inventors: Zhongyuan Shen, Guozheng Zhang, Li xu, Zhifang Zhang, Xijie Guo, Yadong Wei.
Applicant: The Sericultural Research Institute. Chinese Academy of Agricultural Sciences.
RECENT PUBLICATIONS:
1) Dingguo Xia, Yadong Wei, Guozheng Zhang G, Qiaoling Zhao, Yeshun Zhang, Zhonghuai Xiang,
Chen Lu. cDNA cloning, expression, and enzymatic activity of a novel endogenous cellulase from the
beetle, Batocera horsfieldi. Gene. 2013 Feb 1; 514(1): 62-8.
2) Guozheng Zhang, Yadong Wei, Dingguo Xia, Yeshun Zhang, Bo Deng, Ziyu Liu, Jiekui Zhang,
Chengfu Lou. Differentially expressed genes in the white egg 2 mutant of silkworm, Bombyx mori, at
early embryo development stage. African Journal of Biotechnology.21 December 2011; 10
(83):193**-*****
3) Yang Q, Li C, Wei Yadong, Huang W, Wang LX. Expression, glycoform characterization, and
antibody-binding of HIV-1 V3 glycopeptide domain fused with human IgG1-Fc. Bioconjug Chem.
2010 May 19;21(5):875-83.
4) Hu Z, Lee KS, Choo YM, Yoon HJ, Kim I, Wei Ya Dong, Gui ZZ, Zhang GZ, Sohn HD, Jin BR.
Molecular characterization of a phospholipid-hydroperoxide glutathione peroxidase from the
bumblebee Bombus ignitus. Comp Biochem Physiol B Biochem Mol Biol. 2010 Jan;155(1):54-61. Epub
2009 Oct 3
5) Zhongzheng Gui, Bo yeon Kim, Kwang Sik Lee, Ya Dong Wei,, Xijie Guo, Hung Dae Sohn and Brung
Rae Jin. Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning,
gene structure, expression and distribution. Eur. J. Entomol. 105: 567–574, 2008
6) Yadong Wei, Cishan Li, Wei Huang, Bing Li, Scott Strome, Lai-xi Wang. Glyco-engineering of human
IgG-Fc through combined yeast expression and in vitro chemoenzymatic glycosylation. Biochemistry
2008 Sep 30; 47(39):10294-304
7) Lin W, Voskens CJ, Zhang X, Schindler DG, Wood A, Burch E, Wei Yadong, Chen L, Tian G, Tamada
K, Wang LX, Schulze DH, Mann D, Strome SE. Fc dependent expression of CD137 on human NK cells:
insights into “agonistic” effects of anti-CD137 monoclonal antibodies. Blood. 2008 Aug
1;112(3):699-707
8) Wei Ya Dong, Lee KS, Gui ZZ, Yoon HJ, Kim I, Je YH, Lee SM, Zhang GZ, Guo X, Sohn HD, Jin BR.
N-linked glycosylation of a beetle (Apriona germari) cellulase Ag-EGase II is necessary for enzymatic
activity Insect Biochem Mol Biol. 2006 Jun;36(6):435-41.
9) Wei Ya Dong, Lee KS, Gui ZZ, Yoon HJ, Kim I, Zhang GZ, Guo X, Sohn HD, Jin BR Molecular cloning,
expression, and enzymatic activity of a novel endogenous cellulase from the mulberry longicorn
beetle, Apriona germari. Comp Biochem Physiol B Biochem Mol Biol. 2006 Oct; 145(2):220-9
10) Gui ZZ, Lee KS, Kim BY, Choi YS, Wei Ya Dong, Choo YM, Kang PD, Yoon HJ, Kim I, Je YH, Seo SJ,
Lee SM, Guo X, Sohn HD, Jin BR. Functional role of aspartic proteinase cathepsin D in insect
metamorphosis BMC Dev Biol. 2006 Oct 25;6:49.
11) Gui Zhong Zheng, Bo Yeon Kim, Hyung Joo Yoon, Ya Dong Wei, Guo Xijie, Byung Rae Jin and Hung
Dae Shon. Apriona germari larval cuticle protein genes: genomic structure of three cuticle protein
genes and cDNA cloning of a novel cuticle protein. Int. J. Indust. Entomol. Vol. 14, No. 1, 2007, pp.
51~56
12) Wei Ya Dong, Lee SJ, Lee KS, Gui ZZ, Yoon HJ, Kim I, Je YH, Guo X, Sohn HD, Jin BR.
N-glycosylation is necessary for enzymatic activity of a beetle (Apriona germari) cellulase Biochem
Biophys Res Commun. 2005 Apr 1; 329(1):331-6.
13) Wei Yadong, Zhang Guozheng, Studies on detection of microsporidia (Nosema bombycis) by
hybridization in situ, Acta Sericologica Sinica. 2005, 1: 66-70
14) Zhongzheng Gui, Kwang Sik Lee, Yadong Wei, Hyung Joo Yoon, Iksoo Kim, Bryung Rae Jin, Cloning
and mRNA expression of an action cDNA from the mulberry longicorn beetle, Apriona germari. Int. J.
Indust. Entomol . Vol.9, No.2, 2004, pp. 187-191
15) Zhongzheng Gui, Yadong Wei, Byung Rae Jin Molecular cloning of a LIM protein cDNA from the
mulberry longicorn beetle, Apriona germari. Int. J. Indust. Entomol. Vol.9, No.1,2004, pp. 149-153
16) Wei Yadong, Jin Byung Rae, Molecular Cloning of a Profilin cDNA from Bombyx mori, Int. J. Indust.
Entomol. Vol.9, No.1,2004, pp. 123-126
MAJOR SCIENTIFIC RESEARCH SKILLS:
Molecular biology: DNA and RNA extraction and analysis, PCR, real time-PCR, restriction enzyme
digestion, general cloning, cDNA library construction, DNA microarray, site-directed mutagenesis,
Southern blot analysis, Northern blot analysis, the EGFP and luciferase reportor system, expression of
gene in bacteria, yeast, and insect cells (sf9, sf21, BmN, Tn, High Five), transgenesis manipulation...
Glycobiology: Analyses of glycans by Malti-TOF, MS and high performance anion-exchange
chromatography (HPAEC), Glycans manipulation such as release and transfer by enzymes such as
PNGase F, EndoA, EndoM, and Endo H, Functional analyses of glycoproteins...
Protein biochemistry and Proteomics: protein expression and purification in different systems,
SDS-PAGE, native gel, polyclonal antibody production in mouse (mouse injection, dissection and
bleeding), antibody purification, Western blot analyses, ELISA, immunoprecipitation, HPLC,
2D-gel...
Virology and cell biology: virus (BmNPV, AcMNPV, and other viruses) handling, cell culture and
transfection, virus titer determination by real-time PCR or plaque, virus purification, molecular
analysis of virus, In situ hybridization, immunocytochemistry...
Bio-informatics: Database mining, resident and on-line softwares such as DNA-STAR, Clustalw,
BlastN. BlastP, GCG, Seqlab, Gel-Pro Analyzer, SignalP3.0, Sigcleave; sequence retrieval and
comparison, primer design, and phylogenetic analysis…
Soft scientific skills: Protocol optimization and development, technological survey, adaptation of
technologies, trainee supervision, lecture creation, international journal publications and conference
presentations, research project and collaboration management…
HONORS AND AWARDS:
2004 Outstanding Presentation Award International Conference of Sericultural and Entomological
Sciences, South Korea.
Title: N-glycosylation of a beetle (Apriona germari) cellulase is necessary for its enzyme activity
2005 Excellent Presentation Award the 5th Asia-Pacific Congress of Entomology, South Korea
Title: The Role of N-Glycosylation of a Beetle Cellulase
2005 Excellent Prize Poster and Presentation the Conference of Korean Sericulture, South Korea
Title: N-glycosylation of a beetle cellulase II is necessary for its enzymatic activity
2009 Outstanding Poster and Presentation Awards (Third Place Award) 1st International Symposium
on Insect Biotechnology & 2nd Korea-China Joint Symposium on Insect Biotechnology, South Korea.
Title: SSH cDNA library construction and sequence analysis of the autogenesis early embryo in
silkworm, Bombyx mori
SOCIETY MEMBERSHIPS:
Society for Glycobiology (USA)
The Chinese Society of Sericultural Sciences
The South Korean Society of Sericultural Sciences
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