Quality Assurance Project
Resume:
SAAYI KRUSHNA
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Objective:
I'm qualified, competent, forward thinking and committed research
scientist with a proven ability to implement new methods and
standardization of protocols and successful in leading the academic
research projects, now seeking a challenging position in an industry to
expand my research skills and to nurture the leadership skills.
Research experience:
Organization: Northwestern University
Role: Research Associate
Tenure: 2011- to date
Responsibilities:
< To find the role of Adenoviral protein E1A binding to cellular protein
p300 in induction of DNA replication and DNA damage response.
< Studying the gene expression changes in the cell due to expression of
Adenoviral protein E1A
< To understand the E1A oncogene induced rereplication of cellular DNA and
altered DNA replication dynamics.
< Represented the data in various scientific meetings and journal clubs.
< Trained graduate students in their projects and provided hands on
experience to handle various analytical instruments
< Lab management
< Data review and preparation of scientific papers to communicate to
publication.
Organization: Loyola University Medical Center
Role: Research Associate
Tenure: 2010- 2011
Responsibilities:
< Involved in a project where natural and recombinant hirudin antibodies
were evaluated for cross reactivity.
< Responsible for protein profiling of various clinical samples using
SELDI-TOF-MS.
< Actively involved in the quality assurance of various heparin compounds
using HPLC.
< Provided hands on experience to under graduate and graduate students and
mentoring their research projects.
< SOP writing, implementation and data interpretation.
< Data review and preparation of manuscript for publication.
< Review of literature and writing project proposals.
Organization: R&D center, Dayananda Sagar Institutions,
India
Role: Head, Department of Biochemistry
Tenure: 2002- 2003; 2006-2010.
Responsibilities:
< Mentoring student project and data review
< Lab management
< Analytical training
< Writing and implementing SOPs
< Manuscript preparation and data presentation
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Education:
. Ph.D in Biochemistry, Sri Krishnadevaraya.University, India
2010
. M.Phil in Biochemistry, Sri Krishnadevaraya.University, India
2006
. MS in Biochemistry, Madras University, India
2002
. BS in Biological Sciences, Sri Venkateshwara University, India
1998
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Research techniques:
> Basic Microbiology and cell culture techniques (BSL-2 environment)
> Molecular Biology
. Isolation of DNA, RNA and genomic DNA isolation
. Electrophoresis techniques: SDS-PAGE, Agarose Gel Electrophoresis
. Southern Blotting, Western Blotting.
. PCR, RT-PCR
. Cloning and gene expression
. shRNA construction and gene regulation
.
> Immunological and Biochemical
. Flowcytometry (Cell cycle analysis; apoptosis), Enzyme linked
immunosorbent assay (ELISA), Immunoprecipitation,
immunofluorescence.
> Cell and tissue culture
Primary cell culture (Keratinocytes isolation and culture),
secondary mammalian cell cultures, stable cell clines
preparation.
> Transfection and Transduction
> Analytical techniques
. HPLC
. SELDI-TOF-MS
> Animal handling (Rats)
Dissections, blood collection, and IP injections.
Publications:
* Ghata Singhal, Elisabetta Leo, Saayi Krushna Gadham Setty, Yves
Pommier, Bayar Thimmapaya. Adenovirus E1A oncogene induces
rereplication of cellular DNA and alters DNA replication dynamics. J.
Virology, August 2013; 87(15): 8767-8778.
* Abdul Kareem M, Saayi Krushna G, Shaik AH, Maruthi Prasad E and
Lakshmi Devi K. Protective effect of Nutmeg aqueous extract against
experimentally induced hepatotoxicity and oxidative stress in rats.
J. Ayurveda & Integrative Medicine, 2013; 4(4): 216-223.
* G. Saayi Krushna, M. Abdul Kareem, V. Damodara Reddy, P. Padmavathi,
S. Altaf hussain and Lakshmi Devi K. Aegle marmelos fruit extract
attenuates isoproterenol-induced oxidative stress in rats. J Clin
Biochem Nut, May 2012; 50(3): 199-204.
* Nasir S, Zhu H, Saayi Krushna G, Josephine C, Hoppensteadt D and
Fareed J. " Cross-reactivity of varius thrombin products with anti-
rabbit antibodies to bovine, human and recombinant thrombin. Clinical
and Applied Thrombosis/Hemostasis, 2012, 18(3): 243-248.
* Hussain Shaik A, Rasool SN, Abdul Kareem M, Krushna GS, Akhtar PM,
Devi KL. Maslinic acid protects against Isoproterenol-induced
cardiotoxicity in albino Wistar rats. J Med Food, 2012, 15(8): 741-
746.
* Shaik AH, Rasool SN, Vikram Kumar Reddy A, Abdul Kareem M, Saayi
Krushna G, Lakshmi Devi K. Cardioprotective effect of HPLC
standardized ethanolic extract of Terminalia pallida fruits against
Isoproterenol-induced myocardial infarction in albino rats. J
Ethnopharmacol, 2012, May7; 141(1): 33-40.
* G. Saayi Krushna, M. Abdul Kareem, K. Nagamani and K. Lakshmi Devi.
Preventive effect of Aegle marmelos fruit extract on Isoproterenol
induced atherogenesis in rats. Int. J. Pharm. and Technol., 2011, 3
(1): 1934-1945.
* G. Saayi Krushna, M Abdul Kareem and K Lakshmi Devi.
Antidyslipidaemic effect of Aegle marmelos Linn. fruit on
Isoproterenol induced myocardial injury in rats. The Internet Journal
of Pharmacology, 2009, 6(2): (Online).
* M. Abdul Kareem, G. Saayi Krushna, S. Altaf hussain and K.
Lakshmidevi. "Effect of Nutmeg aqueous extract on hyperglycaemia,
hyperlipidaemia and cardiac histology associated with isoproterenol-
induced myocardial infarction in rats". Tropical Journal of
Pharmaceutical Research, 2009, 8(4): 337-344.
* G. Saayi krushna, M. Abdul Kareem and K. Lakshmi Devi.
"Cardioprotective and antilipid peroxidative effect of Aegle marmelos
against isoproterenol induced cardiac stress in rats". International
journal of green pharmacy, 2007, 1(3-4): 13-15, Oct.-Dec.
* Damodara Reddy.V, Saayi Krushna G, Padmavathi. P and Varadacharyulu.
N.Ch. "Effect of Emblica officinalis against alcohol-induced
biochemical changes in plasma and red blood cells of rats". African
Journal of Biochemistry Research, 2007, 1(6): 101-105.
Phone: 1-847-***-****
E-mail: ********@*****.***
1569 S WOLF ROAD APT#103
PROSPECT HEIGHTS
IL-60070
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