High School Project

Curriculum Vitae

Uma Priyanka Pelluri Email:

***************@*****.***

Mobile: 944******* / 984-***-****

Objective:

To work in an environment that provides a challenging and

rewarding

Career ensuring a high level of job satisfaction and ample opportunities.

Academic Qualification

. Completed M.Sc in biotechnology in GITAM university,2013 with CGPA

of 6.69.

. Completed BSc in Bio-Technology from Vasundara Degree college, 2010

with an Aggregate of 67%.

. Completed Intermediate from Atomic Energy Junior College,2007 with an

Aggregate of 76%.

. Completed 10th from Vignan High school, 2005 with an aggregate of

76%.

. Certifications / Trainings Completed:

. Completed Training on Business Communication Skills

. Attended National Science Conference held at GITAM University.

. Completed academic project work on 'PRODUCTION AND OPTIMIZATION OF

SURFACTIN BY LOCAL ISOLATE OF 'BACILLUS' SPECIES' at BIOWORLD

RESEARCH TECHNOLOGIES,HYDERABAD.

Areas of Interest:

. Immunology

. Molecular biology

. Microbiology.

Techniques Known:

. Biochemical techniques.

. Immunological techniques:

. Immunoelectrophoresis

. Western Blotting technique.

. ELISA

. Radial Immunoelectrophoresis.

. Quantitative precipitin assay.

. Double Immunodiffusion test.

. Microbiological techniques:

. Detection of Bacterial Mobility by Hanging Drop Method.

. Antibiotic Sensitivity test by Disc and Well Diffusion method.

. Evaluation of Disinfectant by Phenol coefficient method.

. Oligodynamic action of copper on Bacteria.

. Microbial Estimation of milk by Resazurin test.

. Determination of potability of water by MPN test.

. Determination of Doubling time of E.coli.

. Molecular Biological techniques:

. Restriction Fragment Length polymorphism.

. Random Amplification of polymorphic DNA(RAPD analysis).

. Cloning of GFP gene.

. Amplification of DNA by PCR.

. Southern Blotting technique.

. Northern Blotting technique.

. DNA Denaturation and Hyperchromic effect.

. Isolation of Eukaryotic genomic DNA.

. Ligation of DNA fragments.

. Elution of DNA from agarose gels.

. Isolation of RNA.

. DNA restriction and separation of DNA fragments by agarose gel

electrophoresis.

. Invitro transcription,Bacterial transformation and identification of

transformants.

. Isolation of plasmid DNA.

. Isolation of prokaryotic genomic DNA from bacteria.

. Plant Biotechnology techniques:

. Organogenesis and Regeneration of plants.

. Isolation of protoplast by Mechanical method.

. Polyethylene glycol mediated fusion of protoplast.

. Anther culture and production of Haploids.

. Embryo culture of Maize.

. Establiment of callus cultures from Carrot cambial tissue.

. Animal Biotechnology techniques:

. Cell viability by MTT assay.

. Isolation of Lymphocytes from Blood.

. Preparation of Single cell suspension from spleen.

. Trypsinization of Adherant cells.

. Viability test by Trypan blue staining.

Personal Details:

Father Name : P V K Appa Rao

Date of Birth : 13th March, 1990

Gender : Female.

Marital Status : Single

Languages : English, Hindi and Telugu

Alternate Email : **************@*****.***

Permanent Address : Plot No. 47,: Shank Colony,Kapra, ECIL POST

: Hyderabad - 500 062

Date:

Place: (Uma Priyanka

Pelluri)

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